Synaptogenesis and myelinogenesis in dissociated cerebral cells from rat embryo on polylysine coated surfaces

Yavin, Ziva; Yavin, Ephraïm

doi:10.1007/bf00236881

Cited by 104 publications

(39 citation statements)

References 16 publications

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“…This conclusion is also supported by their ultrastructural characteristics [Yavin and Yavin, 1977] and their ability to extend neuritic processes. For mation of the latter appears to be accom panied by a progressive acquisition of new antigenic determinants ( fig.…”

Section: Discussionsupporting

confidence: 63%

“…On the other hand the im munofluorescent-heterotypic aggregate (fig. 4, upper left corner), which is composed of a layer of cells characterized by their ruffled membrane [Yavin and Yavin, 1977], is exten sively labeled. Figure 4 also shows that in contrast to the ring fluorescence of the neu ronal cell body (arrow), its extended axon containing numerous varicosities is totally unlabeled by anti-embryo brain serum.…”

Section: Resultsmentioning

confidence: 99%

“…Dissociated embryonic brain cells grown in suspension culture [DeLong and Sidman, 1970;Moscona, 1974] or on a solid support [for references see Mandel et al, 1976] resemble in many re spects the cellular segregation and organiza tion into distinct arrays which represents the hallmark of brain histogenesis. Aggregation under culture conditions appears to be an essential step for the establishment of inter cellular cooperativity and may be largely re sponsible for the ability of cells to express their functional properties [Seeds, 1971;God frey et al, 1975], In a previous study we have shown that the aggregation process in surface brain cell cultures predates the appearance of axonal and dendritic extensions as well as the establishment of synaptic contacts and forma tion of myelin [Yavin and Yavin, 1977]. While a great deal of experimental evidence has been accumulated on the histotypic organiza tion of cells in suspension cultures [Garber and Moscona, 1972a,b], little information is presently available as to the cell types com prising the cerebral aggregate as it evolves under surface culture conditions [Benda et a!., 1975].…”

Section: Introductionmentioning

confidence: 99%

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Immunofluorescent Patterns of Dissociated Rat Embryo Cerebral Cells during Development in Surface Culture: Distinctive Reactions with Neurite and Perikaryon Cell Membranes

Yavin¹,

Yavin²

1978

Dev Neurosci

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The presence of antigenic determinants on surface cultures of dissociated cells from rat embryo cerebral tissue has been investigated with the immunofluorescence technique. Antisera raised in rabbits against mature mouse brain reacted with the embryonic cells and enabled the identification of two distinct populations of immunofluorescent cells. One population clearly distinguishable by its ring fluorescence was involved in early cellular reaggregation and neurite formation. A second population which displayed dispersed fluorescence over the whole cell, did not establish intercellular contacts for prolonged periods of time in culture. Indirect evidence suggests that after several days in culture a great portion of the latter population was found in close proximity to the early aggregate, to form an immunofluorescent-heterotypic aggregate. A second antiserum, raised against embryonic rat cerebral cells, displayed immunofluorescent labeling patterns similar to those observed with the mature mouse brain antiserum, when reacted with freshly dissociated cerebral cells. As the cells differentiated in surface culture, distinctive patterns of immunofluorescent reactions developed. Large neuronal cells were labeled on their neuritic cell membranes by the antiserum to adult brain but not by the antiserum to embryonic cells. The results suggest that the anti-mature brain serum possess a class of antibodies which is able to react with antigenic determinants localized specifically on the neuritic plasma membrane.

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Section: Discussionsupporting

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunofluorescent Patterns of Dissociated Rat Embryo Cerebral Cells during Development in Surface Culture: Distinctive Reactions with Neurite and Perikaryon Cell Membranes

Yavin¹,

Yavin²

1978

Dev Neurosci

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“…This could indicate an increased synthesis of ex portable proteins, correlating with the in creased number of synaptic vesicles and of synapses. A finding we have interpreted as a sign of neuronal maturation and advanced development in vitro [9,39,40].…”

Section: Discussionmentioning

confidence: 99%

Low Doses of <i>L</i>-Monosodium Glutamate Promote Neuronal Growth and Differentiation in vitro

Aruffo

Ferszt²,

Hildebrandt³

et al. 1987

Dev Neurosci

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Monosodium glutamate given at a concentration of 5 x 10^–6M to whole-brain dissociated cultures of 18-day-old rats promotes neuronal growth. Neurons are larger due to an increased size of both cytoplasm and nucleus. Rough endoplasmic reticulum is more developed and mitochondria are more abundant. Synaptic vesicles are significantly increased in number with respect to control cultures. Synapses are more abundant and show a more differentiated morphology. These findings are interpreted as evidence for accelerated development secondary to the stimulatory effects of glutamate.

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“…The susoension was in&x&d for 12 rnin at 3?C, dispersed by gent& &petting in basal Eagle's medium (BEM) with 10% fetal calf serum (FCS), and passed through a 200 gm Nylon screen. Surface cultures were prepared from the dissociated cells by pipetting them onto 18 mm glass wverslips previously coated with poly-(D-lysine) (Yavin and Yavin, 1977). The cultures were maintained at 37°C in a 5% C02:95% air incubator.…”

Section: Cell Suspensions and Culturesmentioning

confidence: 99%

Oligodendroglia development in cell culture as monitored with a monoclonal antibody

Collins¹,

Seeds²

1986

J. Neurosci.

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A new marker for young oligodendrocytes has been identified by a monoclonal antibody (mOg-1, IgM isotype) prepared from cerebellar plasma membrane stimulated mouse lymphocytes. mOg-1 reactive cells in the mouse cerebellum first appear at day 19 of gestation. Future white matter layers of fixed sections of neonatal rat cerebellum were labeled with mOg-1. Although EM analysis has shown cell-surface binding by presumptive oligodendroglia in neonatal cerebellum, the antibody does not bind to compact myelin. In cell cultures prepared from L-d-old mice, 1.1% of the cells bound mOg-1 after 3 d in culture, but up to 5% of the cells bound mOg-1 after 2 weeks in culture. Of these same Og-l-positive cells, 69% bound anti-gabtctocerebroside and 65% bound anti-myelin basic protein. After a week in culture Og-l-positive cells often produced lamellar sheets extending a millimeter over the polylysine substratum in the absence of normal myelin formation. mOg-1 recognizes a cell-surface determinant distinct from well-characterized oligodendroglial molecules (galactocerebroside, sulfatide and myelin basic protein) that is expressed early in oligodendrocyte development. The antibody has been used to follow the maturation of oligodendrocytes in cultures of both normal and jimpy mouse cerebellum.The nervous system is a complex network derived from multiple associations between different cell types. The limited number of distinct cell types and the availability of neurological mutants with defective cerebellar histogenesis make the mouse cerebellum an attractive model for studying cell-cell interactions.The development of antibodies toward neural cell-surface molecules could be instrumental in establishing the role of specific determinants in histogenesis. We have used the hybridoma technology of Milstein (1975, 1976) In this report, we describe a monoclonal antibody (mOg-1) reactive with the surface of young oligodendrocytes, prior to the Received Dec. 19, 1985; revised Mar. 4, 1986; accepted Mar. 4, 1986. We woula like to thank Dr. Robert Lasher for his helpful discussions and S.Hat&e for her expert assistance. We thank Drs. Dahl, De.Vellis, Evans, Habig, Qua&s, Ranscht, and Rapport for their generous girt of antisera and tetanus toxin used in these studies. Some of these studies were submitted in partial fulfillment of the requirementi for the Doctor of Philosophy degree from the University of Colorado (1983). This work was su~ucwted in nart bv USPHS Grants NS-098 18and T32-bM-07342.

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Synaptogenesis and myelinogenesis in dissociated cerebral cells from rat embryo on polylysine coated surfaces

Cited by 104 publications

References 16 publications

Immunofluorescent Patterns of Dissociated Rat Embryo Cerebral Cells during Development in Surface Culture: Distinctive Reactions with Neurite and Perikaryon Cell Membranes

Immunofluorescent Patterns of Dissociated Rat Embryo Cerebral Cells during Development in Surface Culture: Distinctive Reactions with Neurite and Perikaryon Cell Membranes

Low Doses of <i>L</i>-Monosodium Glutamate Promote Neuronal Growth and Differentiation in vitro

Oligodendroglia development in cell culture as monitored with a monoclonal antibody

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