Synchrotron microscopic X-ray fluorescence analysis of the effects of chronic arsenic exposure in rat brain

Rubio, Marcelo; Pérez, Roberto D.; Perez, Carlos A.; Eynard, A. H.; Bongiovanni, Guillermina A.

doi:10.1016/j.radphyschem.2007.06.010

Cited by 23 publications

(18 citation statements)

References 20 publications

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“…In particular, alterations in Fe metabolisms might be responsible for the generation of intracellular ROS, which are the cause of oxidative stress 466. To evaluate the potential roles of other trace metals in As toxicity, Rubio et al utilized SXRF to quantify the elemental distributions in brain tissue of Wistar rats that were chronically exposed to 100 ppm of sodium arsenite in drinking water over a period of 30 days 467. Compared to control animals, the amount of As increased while the levels of Cl, K, and Fe decreased in brain tissue; however, the total As concentration ultimately reached a saturation point and diminished with further increased As dose.…”

Section: Microprobe X-ray Fluorescence Imaging Techniquesmentioning

confidence: 99%

In Situ Imaging of Metals in Cells and Tissues

et al. 2009

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Section: Microprobe X-ray Fluorescence Imaging Techniquesmentioning

confidence: 99%

In Situ Imaging of Metals in Cells and Tissues

et al. 2009

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“…The preparation of reference samples with an even distribution of metal elements was complicated (Homma‐Takeda et al ., ; Rubio et al ., ; Watanabe et al ., ). In this study, the reference samples were simply prepared, and a good linear correlation of the Ti intensity with the Ti content was observed, despite the somewhat uneven distribution of Ti in the reference samples.…”

Section: Discussionmentioning

confidence: 97%

Quantitative evaluation of the pulmonary microdistribution of TiO₂ nanoparticles using X‐ray fluorescence microscopy after intratracheal administration with a microsprayer in rats

Shinohara

Kano²,

Senoh³

et al. 2015

J of Applied Toxicology

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The unevenness of pulmonary nanoparticle (NP) distribution, which hinders the establishment of an absolute dose-response relationship, has been described as one of the limitations of intratracheal administration techniques for toxicological assessment of inhaled NPs. Quantification of the NP microdistribution would facilitate the establishment of a concentration-response relationship in localized regions of the lung; however, such quantitative methods have not been reported. Here, we established a quantitative method for evaluating pulmonary TiO2 NP microdistribution in rats using X-ray fluorescence microscopy. Ti intensity in lung sections from rats intratracheally administered 10 mg kg(-1) TiO2 NPs with a microsprayer was measured using X-ray fluorescence with a 100 µm beam size. Ti reference samples were prepared by dropping different concentrations of Ti solutions on glass slide or lung sections of untreated rat. Ti intensity increased linearly with Ti content in the reference samples on both substrates. The detection limit of TiO2 was estimated to be 6.3 ng mm(-2) . The reproducibility was confirmed for measurements done in the short- (2 weeks) and long-term (6 months). The quantitative results of TiO2 NP microdistribution suggested that more TiO2 NPs were distributed in the right caudal and accessory lobes, which are located downstream of the administration direction of the NP suspension, and the lower portion of each lobe. The detection rates of TiO2 NPs were 16.6-25.0%, 5.19-15.6%, 28.6-39.2%, 21.4-38.7% and 10.6-23.2% for lung sections from the right cranial, middle, caudal, accessory and left lobes, respectively.

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“…Measurements were performed in air, whereas X‐ray fluorescence spectra were collected using a Silicon Drift Detector. Samples were set in the horizontal plane at 90° from the incident beam to minimize scattering (conventional incidence geometry: 45–45°), as previously reported . They were positioned in the image plane within an accuracy of 0.5 μm with a three‐axis ( x, y, z ) remote controlled stage.…”

Section: Methodsmentioning

confidence: 99%

“…An interesting alternative is synchrotron radiation X‐ray fluorescence (SRXRF), because brilliant, penetrating, high‐energy X‐rays produced by a synchrotron allow direct trace level analysis of a wide range of elements with high spatial resolution, without destroying the sample. Indeed, we previously demonstrated that the bioaccumulation of toxic elements in the tissues of experimentally exposed animals can be spatially resolved with a resolution up to 20 μm and in few seconds (2D mapping, or 3D imaging) by SRXRF and X‐ray fluorescence computed tomography at the X‐Ray Fluorescence Facility (D09B‐XRF) of the Brazilian Synchrotron Light Laboratory (LNLS) . As well, chemical speciation of elements in biological samples was carried out at the LNLS by synchrotron radiation total reflection X‐ray fluorescence analysis combined with X‐ray absorption near edge spectroscopy (XANES), allowing arsenic speciation without previous extraction procedures .…”

Section: Introductionmentioning

confidence: 99%

“…As well, chemical speciation of elements in biological samples was carried out at the LNLS by synchrotron radiation total reflection X‐ray fluorescence analysis combined with X‐ray absorption near edge spectroscopy (XANES), allowing arsenic speciation without previous extraction procedures . Another advantage of SRXRF lies in that samples can usually be analyzed without pretreatment . This is particularly important when a large number of samples need to be analyzed, as often happens in environmental monitoring .…”

Section: Introductionmentioning

confidence: 99%

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Use of synchrotron radiation X‐ray fluorescence and X‐ray absorption spectroscopy to investigate bioaccumulation, molecular target, and biotransformation of volcanic elements

Lamela

Pérez

et al. 2018

X-Ray Spectrometry

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Because environment pollutants have a strong impact on ecosystems, including human health, methods of their determination and mitigation have received special attention in recent years. Taking advantage of the wide range of data that can be obtained by synchrotron radiation X‐ray fluorescence spectroscopy (SRXRF) in the field of environmental sciences, different instrumental setups were used to study the biological fates of toxic elements in volcanic environments. The elemental composition of plants, algae, and bacteria in Copahue and Domuyo volcanoes from Argentinean Patagonia was determined by SRXRF and the volcanic elements Ti, Fe, and Zn were abundant in these organisms. Interestingly, a high As concentration was found in cyanobacteria (26.2 μg/g) living in As contaminated stream (250 μg/ml). Because arsenic is toxic and human carcinogen, element‐retention capacity, element‐protein associations, and arsenic metabolism in this As resistant organism were analyzed by SRXRF. A high capacity (100–95%) of Ti > Fe > Cr > Sr > Ni > Cu > Mn > Zn > As retention was found after aqueous/alcoholic extraction assisted by ultrasonication. The cyanobacterial proteins were separated by SDS‐PAGE, electro‐transferred to nitrocellulose, and mapped by SRXRF. Defined protein bands containing Ca, Ti, Mn, Fe, and/or Zn were observed. Their ability to metabolize arsenic was revealed by combining SRXRF and X‐ray absorption near edge spectroscopy and Dimethylarsenic was found. Based on results, we speculate that these cyanobacteria could be interesting candidates for water treatment. Finally, we conclude that SRXRF is a valuable tool to study the biological cycle of environmental pollutants, including their accumulation, molecular targets, and metabolism. The SRXRF may also assist in remediation researches.

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Synchrotron microscopic X-ray fluorescence analysis of the effects of chronic arsenic exposure in rat brain

Cited by 23 publications

References 20 publications

In Situ Imaging of Metals in Cells and Tissues

In Situ Imaging of Metals in Cells and Tissues

Quantitative evaluation of the pulmonary microdistribution of TiO₂ nanoparticles using X‐ray fluorescence microscopy after intratracheal administration with a microsprayer in rats

Use of synchrotron radiation X‐ray fluorescence and X‐ray absorption spectroscopy to investigate bioaccumulation, molecular target, and biotransformation of volcanic elements

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Synchrotron microscopic X-ray fluorescence analysis of the effects of chronic arsenic exposure in rat brain

Cited by 23 publications

References 20 publications

In Situ Imaging of Metals in Cells and Tissues

In Situ Imaging of Metals in Cells and Tissues

Quantitative evaluation of the pulmonary microdistribution of TiO2 nanoparticles using X‐ray fluorescence microscopy after intratracheal administration with a microsprayer in rats

Use of synchrotron radiation X‐ray fluorescence and X‐ray absorption spectroscopy to investigate bioaccumulation, molecular target, and biotransformation of volcanic elements

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Quantitative evaluation of the pulmonary microdistribution of TiO₂ nanoparticles using X‐ray fluorescence microscopy after intratracheal administration with a microsprayer in rats