Synergic potential of Pelargonium endlicherianum Fenzl. Essential oil and antibiotic combinations against Klebsiella pneumoniae

Dumlupınar, Berrak; Karatoprak, Gökçe Şeker; Celik, Damla Damar; Gürer, Ümran Soyoğul; Demirci, Betül; Gürbüz, Burçak; Rayaman, Pervin; KURTULUŞ>, Eda Merve

doi:10.1016/j.sajb.2020.08.022

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…Dumlupinar et al [ 20 ] showed detailed cell damage of K. pneumoniae after treatment with a combination of Pelargonium endlicherianum essential oil with gentamicin and cefepime, by using scanning electron microscopy (SEM), resulting in increased efficacy of these antibiotics. In another study [ 19 ], the same authors proved that there was a synergistic effect of ampicillin associated with Pelargonium endlicherianum essential oil on Neisseria meningitidis .…”

Section: Discussionmentioning

confidence: 99%

Synergistic Action of AMX Associated with 1,8-Cineole and Its Effect on the ESBL Enzymatic Resistance Mechanism

et al. 2022

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The purpose of the present study is twofold. First, it aims to evaluate the synergistic action of the ß-lactam antibiotic; AMX is associated with 1,8-cineole on six clinical isolates of ESBL-producing Escherichia coli and Klebsiella pneumoniae strains. Second, it aims to determine the effect this association has on the ESBL enzymatic resistance mechanism. The synergistic action of AMX/1,8-cineole was evaluated using partial inhibitory concentrations (PIC), determined by a microplate, a checkerboard and time–kill assays. The effect of AMX/1,8-cineole associations on the ESBL enzymatic resistance mechanism was evaluated using a new optimized enzymatic assay. This assay was based on the determination of the AMX antibacterial activity when combined with 1,8-cineole (at subinhibitory concentrations) in the presence or absence of the ß-lactamase enzyme toward a sensitive E. coli strain. The results of both checkerboard and time–kill assays showed a strong synergistic action between AMX and 1,8-cineole. The results of the enzymatic assay showed that the combination of AMX with 1,8-cineole notably influences the enzymatic resistance of the reaction by decreasing the affinity of the β-lactam antibiotic, AMX, to the β-lactamase enzyme. All obtained results suggested that the AMX/1,8-cineole association could be employed in therapy to overcome bacterial resistance to AMX while reducing the prevalence of resistance.

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Section: Discussionmentioning

confidence: 99%

Synergistic Action of AMX Associated with 1,8-Cineole and Its Effect on the ESBL Enzymatic Resistance Mechanism

et al. 2022

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“…Taramalı elektron mikroskobu altında S. pneumoniae (A) İşlemsiz hücreler (kontrol), (B) P. endlicherianum uçucu yağı (%0.012 (v/v)), (C) penisilin (500 µg/mL) ve (D) uçucu yağ (%0.012 (v/v) + penisilin (500 µg/mL) Tartışma P. endlicherianum uçucu yağının GK/KS sistemi ile analiz edildiği başka bir çalışmamızda, uçucu yağın ana bileşenleri βburbonen (%15.5), 2-feniletil-2-metilbutirat (%10.5), hekzahidrofarnesil aseton (%7.7), α-pinen (%58), germakren D (%5.3) ve βpinen (%5.1) olarak tespit edilmiştir. 12 Ülkemizde yetişen P. endlicherianum üzerinde toprak üstü kısımları ile yapılmış başka bir çalışmada uçucu yağda germakren-D, β-karyofillen, T-kadinol, α-karyofillen, αpinen, β-pinen ana bileşenler olarak bulunmuştur. 10 Çalışmamızda, P. endlicherianum'dan elde edilen uçucu yağ S. pneumoniae'ye karşı önemli bir antibakteriyel aktivite sergilemiştir.…”

Section: Taramalı Elektron Mikroskobuunclassified

“…[21][22][23][24] Daha önceki çalışmamızda elde edilen P. endilcherianum uçucu yağının bazı antibiyotikler ile kombine kullanımı Klebsiealla pneumoniae'ye karşı sinerjistik etki göstererek bakteri üzerinde antimikrobiyal aktivite tespit edilmiştir. 12 Geri dönüşü olmayan zar hasarının, uçucu yağ bileşenlerinin birikmesinden dolayı hücre zarının asitlenmesi ve protein denatürasyonundan kaynaklandığı ileri sürülmektedir. 25…”

Section: Taramalı Elektron Mikroskobuunclassified

Synergic potential of pelargonium endlicherianum fenzl. essential oil and penicillin combination against streptoccus pneumoniae

Dumlupınar

Karatoprak²

2020

Mersin Üniversitesi Sağlık Bilimleri Dergisi

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Pelargonium endlicherianum uçucu yağının enfeksiyon tedavilerinde sıklıkla kullanılan benzilpenisilin ile kombine edilerek in vitro deney modeli içerisinde Streptoccus pneumoniae'ye karşı antimikrobiyal etkisinin ortaya çıkarılması ve insan lökosit hücreleri üzerindeki fonksiyonun tespit edilmesi amaçlandı. Yöntem: P. endlicherianum uçucu yağının benzilpenisilin ile kombine kullanımının Streptococcus pneumoniae'ye karşı antimikrobiyal etkisi agar difüzyon tekniği kullanılarak ve lökosit hücreleri üzerindeki fagositik fonksiyonları in vitro hücre deney modeli içinde insan lökosit hücresi WBC 264-9C ATCC HB-8902 kullanılarak ortaya çıkarılmıştır. P. endlicherianum uçucu yağ ve benzilpenisilinin bakterisidal etkisi zamana bağlı öldürme yöntemi ile zamana ve antibiyotik yoğunluğuna bağlı olarak dinamik olarak ortaya konuldu. Uçucu yağın tek başına ve antibiyotik ile kombine kullanıldığında bakteriyel dış membrandaki geçirgenliği U.V. spektrofotometre ve morfolojik görüntüsü taramalı elektron mikroskobu ile analiz edildi. Bulgular: Tedavi sonrası 24. saatte, ilaç kombinasyonu sadece uçucu yağ tedavisi ile karşılaştırıldığında, yaşayabilir hücre sayısında azalma olduğu gözlendi. Uçucu yağ+antibiyotik kombinasyonlarında uçucu yağ ve antibiyotik arasında sinerjizm gözlenmiş olup, lökosit hücrelerinin fagositik aktivitesi artarak canlı bakteri sayısında belirgin bir düşüş belirlendi. Sonuç: Çalışmanın sonuçlarına göre S. pneumoniae'ye karşı kullanılan benzilpenisilinin P. endlicherianum uçucu yağı ile kombine kullanımı sonucu antibiyotik etkinliğinin arttığı ve böylece bakterilerin antibiyotiklere karşı direnç geliştirmesi engellenerek etkin bir tedavi sağlanabileceği gösterilmiştir.

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“…[14][15][16] Some studies have identified the phytochemical constituents responsible for these properties, which include flavonoids, tannins, and phenolic compounds. [15,17] However, these studies are limited and therefore extensive investigations are needed to decipher the total composition of different plant parts. Despite possessing certain biological properties, the tests evaluating its effects on enzymes involved in human pathologies, as well as its antioxidant potential, remain limited.…”

Section: Introductionmentioning

confidence: 99%

UHPLC‐ESI‐QTOF‐MS metabolite profiles of different extracts from Pelargonium endlicherianum parts and their biological properties based on network pharmacological approaches

Zengin,

Leyva‐Jiménez,

Fernández‐Ochoa

et al. 2024

Archiv der Pharmazie

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In the present study, we aimed to investigate the chemical profiles and biological activities of different extracts (ethyl acetate, dichloromethane, ethanol, and water) of Pelargonium endlicherianum parts (aerial parts and roots). Free radical scavenging, reducing power, phosphomolybdenum, and metal chelating were assayed for antioxidant properties. To detect enzyme inhibitory properties, cholinesterase, amylase, glucosidase, and tyrosinase were chosen as target enzymes. The ethanol extract of the aerial parts contained higher amounts of total bioactive compounds (120.53 mg GAE/g‐24.46 mg RE/g). The ethanol and water extracts of these parts were tentatively characterized by UHPLC‐ESI‐QTOF‐MS and 95 compounds were annotated. In addition, the highest acetylcholiesterase (3.74 mg GALAE/g) and butyrylcholinesterase (3.92 mg GALAE/g) abilities were observed by the ethanol extract of roots. The water extract from aerial parts exhibited the most pronounced inhibitory effects on multiple cancer cell lines, especially A549 (IC50: 23.2 µg/mL) and HT‐29 (IC50: 27.43 µg/mL) cells. Using network pharmacology, P. endlicherianum compounds were studied against cancer, revealing well‐connected targets such as epidermal growth factor receptor (EGFR), phosphoinositide‐3‐kinase (PI3K), AKT, receptor tyrosine‐protein kinase erbB‐2, and growth factor receptor bound protein 2 (GRB2) with significant impact on cancer‐related pathways. The results could open a new path from natural treasure to functional applications with P. endlicherianum and highlight a new study on other uninvestigated Pelargonium species.

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Synergic potential of Pelargonium endlicherianum Fenzl. Essential oil and antibiotic combinations against Klebsiella pneumoniae

Cited by 13 publications

References 33 publications

Synergistic Action of AMX Associated with 1,8-Cineole and Its Effect on the ESBL Enzymatic Resistance Mechanism

Synergistic Action of AMX Associated with 1,8-Cineole and Its Effect on the ESBL Enzymatic Resistance Mechanism

Synergic potential of pelargonium endlicherianum fenzl. essential oil and penicillin combination against streptoccus pneumoniae

UHPLC‐ESI‐QTOF‐MS metabolite profiles of different extracts from Pelargonium endlicherianum parts and their biological properties based on network pharmacological approaches

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