Synergism of thidiazuron and benzyladenine in axillary shoot formation depends on sequence of application in Miscanthus X ogiformis ?Giganteus?

Nielsen, Jeanette Møller; Hansen, Jürgen; Brandt, Kirsten

doi:10.1007/bf00051586

Cited by 44 publications

(19 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In order to identify cytokinin receptor(s), numerous cytokinin-binding proteins (CBPs) were characterized (Brault et al 1999). Based on an analogy to the animal hormone system, Nielsen et al (1995) proposed a model for cytokinin action in plant cells. Both BA and TDZ can bind to a receptor of cytokinin binding protein.…”

Section: Discussionmentioning

confidence: 99%

Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens

Jonoubi¹,

Mousavi²,

Majd³

et al. 2005

Biologia plant.

View full text Add to dashboard Cite

An efficient system for shoot regeneration and Agrobacterium-mediated gene transfer into Brassica napus was developed through the modification of the culture conditions. Different concentrations of benzyladenine (1.5, 3.0 and 4.5 mg dm -3 ) and thidiazuron (0.0, 0.15 and 0.30 mg dm -3 ) were evaluated for shoot regeneration of 7, 14 and 21-d-old hypocotyl explants. Maximum shoot regeneration frequency was obtained in 21-d-old explants using 4.5 mg dm -3 benzyladenine and 0.3 mg dm -3 thidiazuron. Under above culture condition, the highest percentage of shoot regeneration frequency was 200 %. Agrobacterium-infected explants grown on the selection medium gave rise to transgenic shoots at a frequency of 11.8 %. Transformed shoots rooted when cultured on a medium supplemented with 2 mg dm -3 of indolebutyric acid and 10 mg dm -3 kanamycin. The rooted plantlets were successfully established in the soil and developed fertile flowers and viable seeds. Evidences for transformation were confirmed by GUS assay and PCR analysis.

show abstract

Section: Discussionmentioning

confidence: 99%

Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens

Jonoubi¹,

Mousavi²,

Majd³

et al. 2005

Biologia plant.

View full text Add to dashboard Cite

show abstract

“…They found that explants obtained from 7-day-old seedlings cultured on MS medium containing BA in combination with TDZ (2 mg l −1 each) produced a higher number of shoots than with either cytokinin alone. A model was proposed by Nielsen et al (1995) for cytokinin action in the plant cell. Both BA and TDZ can bind to a receptor, a cytokinin-binding protein (CBP) which has two binding sites.…”

Section: Involvement Of Plant Growth Regulatorsmentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

200

View full text Add to dashboard Cite

Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZapplication in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological (physiological and biochemical) events in cells are induced or enhanced by TDZ, but the mode of action of TDZ is yet unknown. However, varieties of underlying mechanisms were revealed by reports showing how morphogenic events were induced by application of TDZ. Other reports showed that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration. Other possibilities include modification in cell membrane, energy levels, nutrient absorption, transport and assimilation, etc. In this review, recent advancements in TDZ application in plant sciences are discussed.

show abstract

“…In vitro propagation of M. Â giganteus has been conducted by shoot regeneration from axillary nodes and apical meristems followed by in vitro tillering (Nielsen et al, 1993;Nielsen et al, 1995;Lewandowski, 1997), and by plantlet regeneration through somatic embryogenesis of callus induced from shoot apices, leaf sections and immature inflorescence tissues (Holme & Petersen, 1996;Lewandowski, 1997). Previous investigations have focused on optimization of tissue culture conditions to improve the induction rate of the embryogenic callus type, described as a compact opaque white callus, by addition of proline and different carbon sources, and the use of suspension culture methods in M. Â giganteus (Holme et al, 1997;Petersen et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Miscanthus×giganteus plant regeneration: effect of callus types, ages and culture methods on regeneration competence

et al. 2010

View full text Add to dashboard Cite

The perennial rhizomatous grass, Miscanthus Â giganteus is an ideal biomass crop due to its rapid vegetative growth and high biomass yield potential. As a naturally occurring sterile hybrid, M. Â giganteus must be propagated vegetatively by mechanically divided rhizomes or from micropropagated plantlets. Plant regeneration through somatic embryogenesis is a viable approach to achieve large-scale production of plantlets in tissue culture. Effect of the callus types, ages and culture methods on the regeneration competence was studied to improve regeneration efficiency and shorten the period of tissue culture in M. Â giganteus. Shoot-forming calli having a yellow or white compact callus with light-green shoot-like structures showed the highest regeneration frequency. Percentage of shoot-forming callus induction from immature inflorescence explants was 41% on callus induction medium containing 13.6 lM 2,4-D and 0.44 lM benzyladenine (BA). The use of a regeneration medium containing 1.3 lM NAA and 22 lM BA was effective at shortening the incubation period required for plantlet regeneration, with 69% of total regenerated plantlets obtained within 1 month of incubation on regeneration medium. Embryogenic-like callus morphotype could maintain regeneration competency for up to 1 year as suspension cultures. Field grown regenerated plants showed normal phenotypic development with DNA content and plant heights comparable to rhizome propagated plants. Winter survival rates of the regenerated plants planted in 2006 and 2007 at the University of Illinois South Farm, Urbana-Champaign, Illinois, were 78% and 56%, respectively.

show abstract

Synergism of thidiazuron and benzyladenine in axillary shoot formation depends on sequence of application in Miscanthus X ogiformis ?Giganteus?

Cited by 44 publications

References 23 publications

Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens

Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens

Thidiazuron: A multi-dimensional plant growth regulator

Miscanthus×giganteus plant regeneration: effect of callus types, ages and culture methods on regeneration competence

Contact Info

Product

Resources

About