Synergistic and antagonistic hemolytic activities of bacteria of genus Arcanobacterium and CAMP-like hemolysis of Arcanobacterium phocae and Arcanobacterium haemolyticum with Psychrobacter phenylpyruvicus

Ulbegi-Mohyla, H; Hassan, Abdulwahed Ahmed; Kanbar, T.; Alber, Julia M.; Lämmler, Christoph; Prenger‐Berninghoff, Ellen; Weiß, R.; Siebert, Ursula; Zschöck, M.

doi:10.1016/j.rvsc.2009.01.008

Cited by 30 publications

(25 citation statements)

References 12 publications

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“…In similarity to the A. pyogenes reference strains and to previous findings [12] both A. pyogenes strains displayed hemolytic properties on sheep and rabbit blood agar, a synergistic CAMP-like hemolysis with staphylococcal β-hemolysin, Rhodococcus equi and Arcanobacterium hemolyticum indicator strains and, described for the first time in the present study, a synergistic CAMP-like hemolysis with A. phocae .…”

supporting

confidence: 91%

“…The cultures included 11 reference strains of nine species of the genus Arcanobacterium [5,6,12], and the strains A. pyogenes 734 and A. pyogenes 4984 described in the present study. A. pyogenes 734 was isolated from the lung of a bearded dragon ( Pogona vitticeps ) together with γ-hemolytic streptococci, Pseudomonas spp.…”

mentioning

confidence: 99%

“…The A. pyogenes strains were investigated for cultural and biochemical properties using the Api-Coryne test system (bioMérieux, Germany), tablets containing various substrates (Inverness Medical, Germany), and 4-methylumbelliferyl-conjugated substrates (Sigma, Germany). The strains were also investigated for CAMP-like synergistic hemolytic activities, as previously described [6,12]. …”

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confidence: 99%

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Identification ofArcanobacterium pyogenesisolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties

et al. 2010

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The present study was designed to identify phenotypically and genotypically two Arcanobacterium (A.) pyogenes strains isolated by post mortem examinations of a bearded dragon and a gecko. The A. pyogenes strains showed the typical biochemical properties and displayed CAMP-like synergistic hemolytic activities with various indicator strains. The species identity could be confirmed genotypically by amplification and sequencing of the 16S rDNA gene and, as novel target gene, by sequencing of the beta subunit of RNA polymerase encoding gene rpoB, of both strains and of reference strains representing nine species of the genus Arcanobacterium. The species identity of the two A. pyogenes strains could additionally be confirmed by PCR mediated amplification of species specific parts of the 16S-23S rDNA intergenic spacer region, the pyolysin encoding gene plo and by amplification of the collagen-binding protein encoding gene cbpA. All these molecular targets might help to improve the future identification and further characterization of A. pyogenes which, as demonstrated in the present study, could also be isolated from reptile specimens.

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confidence: 91%

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confidence: 99%

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Identification ofArcanobacterium pyogenesisolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties

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“…The majority (80%) of the isolates displayed a strong positive effect in the synergistic CAMP test performed with S. aureus producing beta-haemolysin. However, variable results (36-100%) have been reported for this test previously (Ülbegi-Mohyla et al, 2009;Hijazin et al, 2011;Rzewuska et al, 2012).…”

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confidence: 81%

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

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Wang

Gao

et al. 2016

Acta Veterinaria Hungarica

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Trueperella (T.)pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. In this work, the properties, pathogenesis and phenotypic diversity of T. pyogenes isolates from bovine mastitis were studied. Both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by PCR in all T. pyogenes isolates (n = 50). Using the tissue culture plate method, 90% of T. pyogenes isolates were able to form biofilms. The minimum inhibitory concentrations (MICs) of 13 antimicrobials against T. pyogenes isolates were determined. High susceptibility was observed to rifampin (96%), ampicillin (94%), ciprofloxacin (94%), and penicillin (92%), while low susceptibility was found to trimethoprim-sulphamethoxazole (10%) and bacitracin (2%). The intracellular assay revealed that T. pyogenes isolates had different cytopathogenic effects on cells. The high percentage (28.6%) of T. pyogenes isolates suggests that this bacterium is an important contributor to mastitis. Moreover, the high occurrence of multidrug resistance, biofilm production, intracellular survival, and the temporal dynamics of T. pyogenes interactions are key factors for a better understanding of how immunity acts on infections with these bacteria and how they evade immune surveillance, thus highlighting the need for the prudent use of antimicrobial agents in veterinary medicine.Key words: Trueperella pyogenes, bovine mastitis, biofilm, virulence factor, antimicrobial susceptibility Mastitis is an inflammation of the mammary gland caused by several varieties of bacteria such as Staphylococcus (S.) aureus, S. epidermidis, and strep- † Equal contributors

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“…In addition, A. haemolyticum 2289/09 displayed a reverse CAMP reaction with staphylococcal -hemolysin (data not shown). The hemolytic properties on rabbit blood agar, the CAMP-like reactivities and the reverse CAMP reaction are typical properties of A. haemolyticum (Lämmler and Blobel 1988;Hassan et al 2009;Ülbegi-Mohyla et al 2009). A. haemolyticum 2289/09 additionally displayed a moderate liquefaction of Loeffler medium, showed no cross-reactivity with streptococcal serogroup G-specific antisera and had typical biochemical properties of this species.…”

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confidence: 82%

Identification of Arcanobacterium haemolyticum isolated from postcastrational complications of a horse

et al. 2010

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An Arcanobacterium haemolyticum strain isolated from a postcastrational lesion of a horse was identified phenotypically and genotypically. The latter was performed by sequencing the 16S-23S rDNA intergenic spacer region (ISR), by amplification of the gene encoding A. haemolyticum phospholipase D, by amplification of A. haemolyticum specific parts of ISR-23S rDNA and by amplification of the newly described CAMP factor family protein encoding gene of A. haemolyticum. This indicates (as described previously for seven additional A. haemolyticum strains; Hassan et al. 2009) that A. haemolyticum seems to occur also in infections of horses.

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Synergistic and antagonistic hemolytic activities of bacteria of genus Arcanobacterium and CAMP-like hemolysis of Arcanobacterium phocae and Arcanobacterium haemolyticum with Psychrobacter phenylpyruvicus

Cited by 30 publications

References 12 publications

Identification ofArcanobacterium pyogenesisolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties

Identification ofArcanobacterium pyogenesisolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Identification of Arcanobacterium haemolyticum isolated from postcastrational complications of a horse

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