Synergistic and Multidimensional Regulation of Plasminogen Activator Inhibitor Type 1 Expression by Transforming Growth Factor Type β and Epidermal Growth Factor

Song, Xiaoling; Thalacker, Frederic W.; Nilsen-Hamilton, Marit

doi:10.1074/jbc.m111.338079

Cited by 5 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, Smad2/3 appeared to localize more readily in the nuclei of β1 integrin–deficient 4T1 cells (Figure 4B), which significantly enhanced their Smad3/4 transcriptional activity as compared with that in parental 4T1 cells (Figure 4C). Of interest, we observed that PAI-1 transcripts were similarly induced by TGF-β in both 4T1 derivatives (Figure 4D), suggesting involvement of noncanonical TGF-β in mediating this response (Song et al. , 2012).…”

Section: Resultsmentioning

confidence: 69%

Targeted inactivation of β1 integrin induces β3 integrin switching, which drives breast cancer metastasis by TGF-β

Parvani

Galliher-Beckley

Schiemann

et al. 2013

MBoC

100

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 69%

Targeted inactivation of β1 integrin induces β3 integrin switching, which drives breast cancer metastasis by TGF-β

Parvani

Galliher-Beckley

Schiemann

et al. 2013

MBoC

100

View full text Add to dashboard Cite

show abstract

“… 50 This reporter system is often used to test the transcriptional activity of TGF-β signaling. 51 , 52 , 53 , 54 , 55 …”

Section: Resultsmentioning

confidence: 99%

Follistatin N terminus differentially regulates muscle size and fat in vivo

et al. 2017

View full text Add to dashboard Cite

Delivery of follistatin (FST) represents a promising strategy for both muscular dystrophies and diabetes, as FST is a robust antagonist of myostatin and activin, which are critical regulators of skeletal muscle and adipose tissues. FST is a multi-domain protein, and deciphering the function of different domains will facilitate novel designs for FST-based therapy. Our study aims to investigate the role of the N-terminal domain (ND) of FST in regulating muscle and fat mass in vivo. Different FST constructs were created and packaged into the adeno-associated viral vector (AAV). Overexpression of wild-type FST in normal mice greatly increased muscle mass while decreasing fat accumulation, whereas overexpression of an N terminus mutant or N terminus-deleted FST had no effect on muscle mass but moderately decreased fat mass. In contrast, FST-I-I containing the complete N terminus and double domain I without domain II and III had no effect on fat but increased skeletal muscle mass. The effects of different constructs on differentiated C2C12 myotubes were consistent with the in vivo finding. We hypothesized that ND was critical for myostatin blockade, mediating the increase in muscle mass, and was less pivotal for activin binding, which accounts for the decrease in the fat tissue. An in vitro TGF-beta1-responsive reporter assay revealed that FST-I-I and N terminus-mutated or -deleted FST showed differential responses to blockade of activin and myostatin. Our study provided direct in vivo evidence for a role of the ND of FST, shedding light on future potential molecular designs for FST-based gene therapy.

show abstract

“…Methods for labeling and purifying newly synthesized RNA amenable to high-throughput downstream analyses have been employed in a variety of eukaryotes using either 4-thiouracil (4tU) or 4-thiouridine (4sU) [22], [24], [28], [29], [30], [31], [32], [33], [34], [35]. Incorporation of 4tU requires the activity of uracil phosphoribosyltransferase (UPRT), a key enzyme in the pyrimidine salvage pathway for recycling uracil to uridine monophosphate (UMP).…”

Section: Resultsmentioning

confidence: 99%

“…An alternative approach is to biosynthetically label newly synthesized RNA with 4-thiouracil or 4-thiouridine, which are readily incorporated and do not impact message stability (reviewed in [28]). Thiol-specific biotinylation, followed by streptavidin-coated magnetic bead separation, enables total RNA to be separated into pools of pre-existing and newly synthesized (thiolated) RNA that are amenable to transcriptome-level downstream applications such as microarrays or RNA-seq [22], [24], [28], [29], [30], [31], [32], [33], [34], [35]. In addition to providing a means to identify transcriptionally activated genes, global RNA half-lives can be determined, capitalizing on the fact that newly synthesized RNA and pre-existing RNA pools sum to the total RNA pool.…”

Section: Introductionmentioning

confidence: 99%

Global Analysis of mRNA Half-Lives and de novo Transcription in a Dinoflagellate, Karenia brevis

Morey

Dolah

2013

PLoS ONE

View full text Add to dashboard Cite

Dinoflagellates possess many physiological processes that appear to be under post-transcriptional control. However, the extent to which their genes are regulated post-transcriptionally remains unresolved. To gain insight into the roles of differential mRNA stability and de novo transcription in dinoflagellates, we biosynthetically labeled RNA with 4-thiouracil to isolate newly transcribed and pre-existing RNA pools in Karenia brevis. These isolated fractions were then used for analysis of global mRNA stability and de novo transcription by hybridization to a K. brevis microarray. Global K. brevis mRNA half-lives were calculated from the ratio of newly transcribed to pre-existing RNA for 7086 array features using the online software HALO (Half-life Organizer). Overall, mRNA half-lives were substantially longer than reported in other organisms studied at the global level, ranging from 42 minutes to greater than 144 h, with a median of 33 hours. Consistent with well-documented trends observed in other organisms, housekeeping processes, including energy metabolism and transport, were significantly enriched in the most highly stable messages. Shorter-lived transcripts included a higher proportion of transcriptional regulation, stress response, and other response/regulatory processes. One such family of proteins involved in post-transcriptional regulation in chloroplasts and mitochondria, the pentatricopeptide repeat (PPR) proteins, had dramatically shorter half-lives when compared to the arrayed transcriptome. As transcript abundances for PPR proteins were previously observed to rapidly increase in response to nutrient addition, we queried the newly synthesized RNA pools at 1 and 4 h following nitrate addition to N-depleted cultures. Transcriptome-wide there was little evidence of increases in the rate of de novo transcription during the first 4 h, relative to that in N-depleted cells, and no evidence for increased PPR protein transcription. These results lend support to the growing consensus of post-transcriptional control of gene expression in dinoflagellates.

show abstract

Synergistic and Multidimensional Regulation of Plasminogen Activator Inhibitor Type 1 Expression by Transforming Growth Factor Type β and Epidermal Growth Factor

Cited by 5 publications

References 48 publications

Targeted inactivation of β1 integrin induces β3 integrin switching, which drives breast cancer metastasis by TGF-β

Targeted inactivation of β1 integrin induces β3 integrin switching, which drives breast cancer metastasis by TGF-β

Follistatin N terminus differentially regulates muscle size and fat in vivo

Global Analysis of mRNA Half-Lives and de novo Transcription in a Dinoflagellate, Karenia brevis

Contact Info

Product

Resources

About