Synergistic Anticancer Activity of 5-Aminolevulinic Acid Photodynamic Therapy in Combination with Low-dose Cisplatin on Hela Cells

Wei, Xiaoqiang; Ma, Haiying; Liu, Aihong; Zhang, Youzhong

doi:10.7314/apjcp.2013.14.5.3023

Cited by 29 publications

(24 citation statements)

References 30 publications

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“…It was also shown that PDT in combination with cisplatin resulted in a considerably increased in vitro and in vivo antitumor effect, which may prove very useful considering the often dose-limiting toxicity of cisplatin. 40,41 One study also examined the combination of PDT, chemotherapy, and adoptive immunotherapy with splenic lymphocytes, adding a further dimension to the combination approach. 47 This triple combination proved effective in treating an aggressive murine leukemia model in vivo, where either PDT alone or chemotherapy alone was ineffective.…”

Section: Photodynamic Therapy In Combination With Established Anticanmentioning

confidence: 99%

Photodynamic Therapy and Its Role in Combined Modality Anticancer Treatment

Brodin

Guha

Tomé

2014

Technol Cancer Res Treat

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Photodynamic therapy (PDT) is a relatively new modality for anticancer treatment and although the interest has increased greatly in the recent years, it is still far from clinical routine. As PDT consists of administering a nontoxic photosensitizing chemical and subsequently illuminating the tumor with visible light, the treatment is not subject to dose-limiting toxicity, which is the case for established anticancer treatments like radiation therapy or chemotherapy. This makes PDT an attractive adjuvant therapy in a combined modality treatment regimen, as PDT provides an antitumor immune response through its ability to elicit the release of damage-associated molecular patterns and tumor antigens, thus providing an increased antitumor efficacy, potentially without increasing the risk of treatment-related toxicity. There is great interest in the elicited immune response after PDT and the potential of combining PDT with other forms of treatment to provide potent antitumor vaccines. This review summarizes recent studies investigating PDT as part of combined modality treatment, hopefully providing an accessible overview of the current knowledge that may act as a basis for new ideas or systematic evaluations of already promising results.

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Section: Photodynamic Therapy In Combination With Established Anticanmentioning

confidence: 99%

Photodynamic Therapy and Its Role in Combined Modality Anticancer Treatment

Brodin

Guha

Tomé

2014

Technol Cancer Res Treat

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“…PDT can be used in combination with a variety of currently used cancer therapies, including chemotherapy, 4, 5 radiation therapy, 6 surgery, 7 gene therapy, 8 and immunotherapy, 9 without compromising these therapeutic modalities. Moreover, the adverse effects of chemotherapy or radiotherapy are absent, and considering its unique 1 O 2 -dependent cytotoxic effects PDT can be safely combined without the risk of inducing cross-resistance.…”

mentioning

confidence: 99%

Two combined photosensitizers: a goal for more effective photodynamic therapy of cancer

et al. 2014

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Photodynamic therapy (PDT) is a clinically approved therapeutic modality for the treatment of diseases characterized by uncontrolled cell proliferation, mainly cancer. It involves the selective uptake of a photosensitizer (PS) by neoplastic tissue, which is able to produce reactive oxygen species upon irradiation with light, leading to tumor regression. Here a synergistic cell photoinactivation is reported based on the simultaneous administration of two PSs, zinc(II)-phthalocyanine (ZnPc) and the cationic porphyrin meso-tetrakis(4-N-methylpyridyl)porphine (TMPyP) in three cell lines (HeLa, HaCaT and MCF-7), using very low doses of PDT. We detected changes from predominant apoptosis (without cell detachment) to predominant necrosis, depending on the light dose used (2.4 and 3.6 J/cm2, respectively). Analysis of changes in cytoskeleton components (microtubules and F-actin), FAK protein, as well as time-lapse video microscopy evidenced that HeLa cells were induced to undergo apoptosis, without losing adhesion to the substrate. Moreover, 24 h after intravenous injection into tumor-bearing mice, ZnPc and TMPyP were preferentially accumulated in the tumor area. PDT with combined treatment produced significant retardation of tumor growth. We believe that this combined and highly efficient strategy (two PSs) may provide synergistic curative rates regarding conventional photodynamic treatments (with one PS alone).

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“…Fotosensitizerin, 24 saatlik inkübasyon ardından SKOV-3 hücrelerine karşı 100 µM'a kadar toksisite göstermediği ancak 5J şiddetindeki ışıkla aktive edildiğinde ise konsantrasyonla doğru orantılı olarak fototoksisitesinde artış olduğu görüldü. Yapılan detaylı testler sonucu IC50 değeri 79 µM olarak belirlenmiştir (18).…”

Section: Bulgularunclassified

“…FDT'nin over kanseri üzerindeki farklı hücre hatlarında çalışmaları umut verici olmakla birlikte tek doz FDT ile çok yüksek oranlarda mortalite elde edilemediği görülmüştür (11)(12)(13)(14)(15). FDT'nin cerrahi, hipertermi, imunoterapi veya kemoterapi ile kombinasyon terapi çalışmalarının in vitro ve in vivo deneylerle tek tedavi yöntemine kıyasla daha başarılı olduğu saptanmıştır (16)(17)(18). Bu durum FDT'nin farklı tedavi yöntemleri ile birleştirilerek geliştirilecek yeni kombinasyon terapi çalışmalarının gerekli ve umut verici olduğunu göstermektedir.…”

Section: Introductionunclassified

Over Kanseri̇ne Yöneli̇k Fotodi̇nami̇k Terapi̇ Temelli̇ Kombi̇nasyon Terapi̇ Uygulamasi

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et al. 2018

Zeynep Kamil Tıp Bülteni

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ÖZETKanser, tüm dünyada olduğu gibi ülkemizde de en ölümcül hastalıkların başında gelmektedir. Kanserin, lokal, minimum yan etkiyle ve hedefe yönelik olarak tedavisi hasta için büyük önem taşımaktadır. Fotodinamik Terapi (FDT), lokal, fotokimyaya dayalı ve yan etkisi minimum olan bir tedavi yöntemidir. Özetle FDT, ışıkla aktive olan ilaç (fotosensitizer), ışık ve oksijenin birleşmesi sonucu ortaya çıkan serbest radikallerin ve/veya tekil (singlet) oksijenin hücrede bir dizi zincir reaksiyon başlatarak hücre ölümüne sebep olması prensibine dayanır. Oksijen, ışık ve fotosensitizer tek başına herhangi bir toksik etki göstermeyip, ancak bir araya geldiklerinde başlayan fotokimyasal reaksiyonlar sonucu oluşan sitotoksisite sebebiyle hedeflenen hücrenin ölümü gerçekleşir. FDT'nin en önemli bileşeni hücre içinde, ışık enerjisini kimyasal enerjiye dönüştüren araç olan fotosensitizerdir. FDT'nin etkinliği farklı terapi modelleri ile birleştirilerek arttırılabilir. Kanser gibi dejeneratif hastalıkların genellikle birden fazla patolojik mekanizma içerdiklerini ve bu hastalıklarla tek tedavi yöntemiyle savaşmanın yeterli olmayacağını akılda tutmak gerekir. Bu nedenle iki ya da daha fazla tedavi yönteminin eş zamanlı olarak kullanılması başarıyı arttırmaktadır. Kombinasyon terapinin amacı kullanılan tedavi yöntemleri ve/veya ilaç ile hücredeki farklı sinyal yolaklarını dolayısı ile farklı hücre hasarı yollarını hedef alarak birden fazla mekanizmayı aktive ederek kanser hücresinin yok edilmesi ihtimalini arttırmaktır. Bu bilgilerin ışığında FDT ve kemoterapiden oluşan yeni kombinasyon terapinin SKOV-3 over kanser hücre hattında etkisi araştırıldı. Özgün, suda çözünürlüğü yüksek fotosensitizer ile klinikte sıklıkla kemoterapi ilacı olarak kullanılan İrinotekan kullanıldı. FDT ve kemoterapiden oluşan kombinasyon terapinin uygulanan tedavinin sırasına bağlı olarak çok büyük değişiklik gösterdiği görüldü. Örneğin, FDT'nin önce uygulandığı durumda belirli ilaç dozlarında güçlü antagonist etki görüldü. Öte yandan, aynı ilaç konsantrasyonlarında yalnızca uygulama sırası değiştirilerek sinerjik etkinin elde edilebileceği görüldü. Anahtar Kelimeler: fotodinamik terapi, over kanseri, SKOV-3, kombinasyon terapi, toksisite, ROS, irinotekan ABSTRACTCancer is one of the most leading causes of death in Turkey as well as in the world. It is very critical to cure the cancer via targeted therapy that is locally applied and shows minimum side effects for patient's life quality. Photodynamic therapy (PDT) is a local, photochemistry based treatment, which has minimum side effects. In brief, PDT is a result of a combination of photosensitizer, a drug that is activated by light, light and oxygen that generates free radicals and/or singlet oxygen and starts series of reactions in cell and eventually cause cell death. While light, oxygen and photosensitizer has no cytotoxic effect alone, combination of three starts all photochemical reactions and as a result cytotoxicity is generated in the tissue. Photosensitizer, converting light energy to the chem...

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Synergistic Anticancer Activity of 5-Aminolevulinic Acid Photodynamic Therapy in Combination with Low-dose Cisplatin on Hela Cells

Cited by 29 publications

References 30 publications

Photodynamic Therapy and Its Role in Combined Modality Anticancer Treatment

Photodynamic Therapy and Its Role in Combined Modality Anticancer Treatment

Two combined photosensitizers: a goal for more effective photodynamic therapy of cancer

Over Kanseri̇ne Yöneli̇k Fotodi̇nami̇k Terapi̇ Temelli̇ Kombi̇nasyon Terapi̇ Uygulamasi

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