Synergistic Antifungal Activity of Chitinolytic Enzymes and Prodigiosin Produced by Biocontrol Bacterium, Serratia marcescens Strain B2 against Gray Mold Pathogen, Botrytis cinerea

Someya, Nobutaka; Nakajima, Masami; Hirayae, Kazuyuki; Hibi, Tadaaki; Akutsu, Katsumi

doi:10.1007/pl00013038

Cited by 116 publications

(94 citation statements)

References 15 publications

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“…It has been reported that a strain of Serratia marcescens and a chitinase gene-transfected Erwinia ananas showed biocontrol activity against various phytopathogens, the biocontrol being related to their chitinolytic activities (30)(31)(32). Therefore, highly chitinolytic isolates including GP1 and GP2 might have the potential to control phytopathogens.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the chitinolytic organisms produce multiple chitinases (6,31). Therefore, chitinolytic activity depends on various factors such as the number of isozymes, their activity, and amounts of the enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, chitinolytic activity depends on various factors such as the number of isozymes, their activity, and amounts of the enzymes. Synergistic effects of multiple chitinases have been reported for both chitin degradation and antifungal activity (8,31,35). Another regulatory mechanism of chitinolytic activity has recently been revealed in Gram-negative bacteria such as C. violaceum and Pseudomonas aeruginosa; in these bacteria, chitinolytic activity is regulated by quorum sensing via the signal molecule AHL (6,38).…”

Section: Discussionmentioning

confidence: 99%

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Diversity of Culturable Chitinolytic Bacteria from Rhizospheres of Agronomic Plants in Japan

et al. 2011

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A total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16S rRNA gene sequences of these isolates were determined. Phylogenetic analyses revealed that 81 isolates belonged to the classes Betaproteobacteria (39 isolates) and Gammaproteobacteria (42 isolates). Of the remaining 19 isolates, 16 belonged to the phylum Firmicutes. Clustering analysis identified 6 and 3 operational taxonomic units (OTUs) in Gammaproteobacteria and Betaproteobacteria, respectively, at the genus level. The majority of chitinolytic bacteria in Gammaproteobacteria belonged to the genera Serratia, Stenotrophomonas, and Lysobacter (14, 15, and 7 isolates, respectively) while those in Betaproteobacteria belonged to the genus Mitsuaria (37 isolates). The 16 isolates placed in Firmicutes belonged to 2 genera, Paenibacillus and Bacillus (8 isolates each). The isolates in the remaining OTUs belonged to the genera Erwinia, Aeromonas, Pseudomonas, Achromobacter, Flavobacterium, and Microbacterium, in less abundance. These results showed a wide distribution of culturable chitinolytic bacteria in the rhizospheres of various agronomic plants. Considering the potential antagonistic activity of chitinolytic enzymes against phytopathogenic fungi, which is exhibited by fungal cell wall degradation, the above-mentioned native chitinolytic bacteria in rhizospheres could potentially be utilized for the biological control of soil-borne phytopathogenic fungi.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diversity of Culturable Chitinolytic Bacteria from Rhizospheres of Agronomic Plants in Japan

et al. 2011

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“…Strain DUT of Serratia marcescens secreted in broth a 70 kDa protein similar to chitinase reported by Someya et al (2001). The extracellular protein of the strain DUT was ultrafiltrated using 40 kDa filtration membranes and was detected using the SDS-PAGE.…”

Section: Resultsmentioning

confidence: 99%

Isolation and identification of a novel endophytic bacterial strain with antifungal activity from wild blueberryVaccinium uliginosum

Xia

et al. 2007

Ann. Microbiol.

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A strain of endophytic bacterium with a good anti-fungal ability was first isolated from Vaccinium uliginosum. It was identified as Serratia marcescens using the 16s rDNA sequence homology and through its physiological and biochemical characteristics. Through the alignment and cladistic analysis of homologous nucleotide sequences of known Serratia marcescens, it was found to be a novel subspecies of S. marcescens. An extensive suppressive spectrum of this endophytic strain of S. marcescens against plant pathogenic fungi was observed. In particular, it inhibited the growth of the causal agents of blueberry leaf spot and of other ten plant fungal pathogens. The inhibition rate, among the different fungi, ranged from 22.7-70.5%. The study indicated the potential use of this S. marcescens endophytic strain in the biological control of blueberry's leaf spot as well as many other plant diseases. To our knowledge, this was the first time that an endophytic S. marcescens was isolated from a wild blueberry in China, and may also represent a novel introduction to the world.

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“…We have previously reported that a chitinolytic enzyme-producing bacterium, Serratia marcescens, controlled some fungal plant diseases (Someya et al, 2000;2005a), and demonstrated that a low dosage of antifungal compounds including antibiotic and chemical fungicides enhanced the antifungal activity of the chitinase-producing bacterium (Someya et al, 2001;2005c). In the cases of combined application of lytic enzymes and antifungal compounds, cell wall degradation by lytic enzymes has a possibility to enhance the uptake of antifungal compounds into fungal cells (Someya et al, 2001;2005c;Woo et al, 2002). However, although strain LRB3W1 does not produce lytic enzymes such as chitinases, synergistic biocontrol activity was observed.…”

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confidence: 99%

Combined Use of the Biocontrol Bacterium Pseudomonas fluorescens Strain LRB3W1 with Reduced Fungicide Application for the Control of Tomato Fusarium Wilt

et al. 2006

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Synergistic Antifungal Activity of Chitinolytic Enzymes and Prodigiosin Produced by Biocontrol Bacterium, Serratia marcescens Strain B2 against Gray Mold Pathogen, Botrytis cinerea

Cited by 116 publications

References 15 publications

Diversity of Culturable Chitinolytic Bacteria from Rhizospheres of Agronomic Plants in Japan

Diversity of Culturable Chitinolytic Bacteria from Rhizospheres of Agronomic Plants in Japan

Isolation and identification of a novel endophytic bacterial strain with antifungal activity from wild blueberryVaccinium uliginosum

Combined Use of the Biocontrol Bacterium Pseudomonas fluorescens Strain LRB3W1 with Reduced Fungicide Application for the Control of Tomato Fusarium Wilt

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