Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium

Meyer-Cifuentes, Ingrid; Werner, Johannes; Jehmlich, Nico; Will, Sabine Eva; Neumann‐Schaal, Meina; Öztürk, Başak

doi:10.1038/s41467-020-19583-2

Cited by 155 publications

(91 citation statements)

References 89 publications

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“…Use of consortium is also very beneficial in synergistic biodegradation of aromatic-aliphatic copolyester plastic. This consortium was isolated from marine ecosystem and have the tendency to use pollutant as sole carbon source in 15 days 43 .…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies

Farhan

Ahmad

Kanwal³

et al. 2021

Sci Rep

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Extensive pesticides use is negatively disturbing the environment and humans. Pesticide bioremediation with eco-friendly techniques bears prime importance. This study evaluates the bioremediation of chlorpyrifos in soil using indigenous Bacillus cereus Ct3, isolated from cotton growing soils. Strains were identified through ribotyping (16s rRNA) by Macrogen (Macrogen Inc. Geumchen-gu, South Korea). Bacillus cereus Ct3 was resistant up to 125 mg L−1 of chlorpyrifos and successfully degraded 88% of chlorpyfifos in 8 days at pH 8. Bacillus cereus Ct3 tolerated about 30–40 °C of temperature, this is a good sign for in situ bioremediation. Green compost, farmyard manure and rice husk were tested, where ANOVA (P < 0.05) and Plackett–Burman design, results indicated that the farm yard manure has significant impact on degradation. It reduced the lag phase and brought maximum degradation up to 88%. Inoculum size is a statistically significant (P < 0.05) factor and below 106 (CFU g−1) show lag phase of 4–6 days. Michaelis–Menten model results were as follows; R2 = 0.9919, Vmax = 18.8, Ks = 121.4 and Vmax/Ks = 0.1546. GC–MS study revealed that chlorpyrifos first converted into diethylthiophosphoric acid and 3,5,6-trichloro-2-pyridinol (TCP). Later, TCP ring was broken and it was completely mineralized without any toxic byproduct. Plackett–Burman design was employed to investigate the effect of five factors. The correlation coefficient (R2) between experimental and predicted value is 0.94. Central composite design (CBD) was employed with design matrix of thirty one predicted and experimental values of chlorpyrifos degradation, having “lack of fit P value” of “0.00”. The regression coefficient obtained was R2 = 0.93 which indicate that the experimental vales and the predicted values are closely fitted. The most significant factors highlighted in CBD/ANOVA and surface response plots were chlorpyrifor concentration and inoculum size. Bacillus cereus Ct3 effectively degraded chlorpyrifos and can successfully be used for bioremediation of chlorpyrifos contaminated soils.

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Section: Resultsmentioning

confidence: 99%

Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies

Farhan

Ahmad

Kanwal³

et al. 2021

Sci Rep

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“…I. sakaiensis can further degrade Te via a TPD cluster. Among the different Mles identified in our previous study (Meyer-Cifuentes et al, 2020), only Mle046, presumably produced by an Alphaproteobacterial strain, was consistently expressed and produced during PF degradation. This mle046 is 100% identical to a homolog present in Celeribacter manganoxidans and its protein is 46.9% identical to IsMHETase.…”

Section: Introductionmentioning

confidence: 62%

“…The Mle046 sequence obtained in a previous study (Meyer-Cifuentes et al, 2020) was used in this study and codon optimized for recombinant expression. The theoretical size of the His-tagged Mle046 is 62 kD.…”

Section: Mle046 Purification and Identificationmentioning

confidence: 99%

“…Their fate and biodegradability in aquatic natural environments, however, are still poorly understood. In a previous study, we revealed that a synergistic mechanism within a marine consortium was necessary to achieve complete mineralization of a PBAT-based polymer blend (PF) (Meyer-Cifuentes et al, 2020). In that study, several putative PETase-like (Ple) and MHETase-like (Mle) hydrolases as well as terephthalic acid degrading genes (TPD) were found in the metagenome.…”

Section: Introductionmentioning

confidence: 99%

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Mle046 Is a Marine Mesophilic MHETase-Like Enzyme

Meyer-Cifuentes

Öztürk

2021

Front. Microbiol.

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Accumulation of plastics in the oceans presents a major threat to diverse ecosystems. The introduction of biodegradable plastics into the market aims to alleviate the ecological burden caused by recalcitrant plastics. Poly (butylene adipate-co-terephthalate) (PBAT) is a biodegradable commercial plastic that can be biodegraded similarly to polyethylene terephthalate (PET) by PETase-like enzymes and MHETases. The role of MHETases is to hydrolyze the intermediate degradation product of PET, mono-2-hydroxyethyl terephthalate (MHET) to its monomers. We recently identified a homolog of the MHETase of the PET-degrading bacterium Ideonella sakaiensis, Mle046, from a marine microbial consortium. In this consortium, Mle046 was highly expressed when a PBAT-based blend film (PF) was supplied as the sole carbon source. In this study, we recombinantly expressed and biochemically characterized Mle046 under different conditions. Mle046 degrades MHET but also 4-(4-hydroxybutoxycarbonyl) benzoic acid (Bte), the intermediate of PF degradation. Mle046 is a mesophilic enzyme adapted to marine conditions, which rapidly degrades MHET to terephthalate and ethylene glycol at temperatures between 20 and 40°C. Mle046 degradation rates were similar for Bte and MHET. Despite its mesophilic tendency, Mle046 retains a considerable amount of activity at temperatures ranging from 10 to 60°C. In addition, Mle046 is active at a range of pH values from 6.5 to 9. These characteristics make Mle046 a promising candidate for biotechnological applications related to plastic recycling.

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“…In recent years, considerable progress concerning plastic polymers with hydrolysable groups in their backbones, as PET, PA, or PUR were reported, obtained mainly by polyaddition or polycondensation. Several studies described the ability of microorganisms and enzymes to degrade these plastics [33][34][35][36][37][38][39][40][41][42][43][44][45][46]. Typical enzymes are cutinases, lipases, and carboxylesterases [47].…”

Section: Microbial and Enzymatic Plastics Biotransformationmentioning

confidence: 99%

MIXed Plastics Biodegradation and UPcycling Using Microbial Communities: The EU Horizon 2020 Project MIX-UP

Ballerstedt

Tiso

Wierckx

et al. 2021

Preprint

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This article introduces the EU Horizon 2020 research project MIX-UP, “Mixed plastics biodegradation and upcycling using microbial communities”. The project focuses on the ambitious vision to change the traditional linear value chain of plastics to a sustainable, biodegradable based one. In MIX-UP, plastic mixtures containing five of the top six fossil-based recalcitrant plastics (PE, PUR, PP, PET, and PS), along with upcoming biobased and biodegradable plastics (bioplastics) such as PHA and PLA, will be used as feedstock for microbial transformations. The generated new workflow increases recycling quotas and adds value to present poorly recycled plastic waste streams. Consecutive controlled enzymatic and microbial degradation of mechanically pre-treated plastics waste combined with subsequent microbial conversion to polymers and value-added chemicals by mixed cultures. Through optimization of known plastic-degrading enzymes by integrated protein engineering, high specific binding capacities, stability, and catalytic efficacy towards a broad spectrum of plastic polymers under high salt content and temperature conditions will be achieved. Another focus lies in the search and isolation of novel enzymes active on recalcitrant polymers. MIX-UP will also enhance the production of enzymes and formulate enzyme cocktails tailored to specific waste streams. In vivo and in vitro application of these cocktails enables stable, self-sustaining microbiomes to convert the released plastic monomers selectively into value-added products, key building blocks, and biomass. Any of the remaining material recalcitrant to the enzymatic activity will be recirculated into the process by physicochemical treatment. The Chinese-European MIX-UP is a multidisciplinary and industry-participating consortium to address the market need for novel sustainable routes to valorize plastic waste streams. MIX-UP realizes a circular (bio) plastic economy and will contribute where mechanical and chemical plastic recycling show limits.

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Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium

Cited by 155 publications

References 89 publications

Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies

Biodegradation of chlorpyrifos using isolates from contaminated agricultural soil, its kinetic studies

Mle046 Is a Marine Mesophilic MHETase-Like Enzyme

MIXed Plastics Biodegradation and UPcycling Using Microbial Communities: The EU Horizon 2020 Project MIX-UP

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