Synergistic combination therapy of lung cancer using paclitaxel- and triptolide-coloaded lipid–polymer hybrid nanoparticles

Abstract

PurposeNon-small cell lung cancer (NSCLC) accounts for the majority of lung cancer. Lipid–polymer hybrid nanoparticles (LPNs) combine the advantages of both polymeric nanoparticles and liposomes into a single delivery platform. In this study, we engineered LPNs as the co-delivery system of paclitaxel (PTX) and triptolide (TL) to achieve synergistic therapeutic effect and reduced drug resistance.Materials and methodsIn this study, PTX- and TL-coloaded LPNs (P/T-LPNs) were fabricated by nanoprecipitation method using lipid and polymeric materials. The P/T-LPNs combination effects on human lung cancer cells were studied. Therapeutic potentials of P/T-LPNs were further determined using lung cancer cells-bearing mice model.ResultsThe average particle sizes of LPNs were around 160 nm, with narrow size distribution below 0.2. The zeta potential value of LPNs was about −30 mV. The encapsulating efficiency (EE) of PTX and TL loaded in LPNs was over 85%. The cytotoxicity of dual drug loaded LPNs was higher than single drug loaded LPNs. The combination therapy showed synergistic when PTX:TL weight ratio was 5:3, indicating the synergy effects of the LPNs. In vivo tumor growth curve of the experimental group was more gentle opposed to the control group, and tumor volumes of P/T-LPNs and control group were 392 and 1,737 mm3, respectively. The inhibition rate on day 20 was 77.4% in the P/T-LPNs group, which is higher than the free drugs solution.ConclusionThe in vivo and in vitro results proved the synergetic effect of the two drugs coloaded in LPNs on the lung cancer xenografts, with the least systemic toxic side effect.

<p>Combination Treatment of Cervical Cancer Using Folate-Decorated, pH-Sensitive, Carboplatin and Paclitaxel Co-Loaded Lipid-Polymer Hybrid Nanoparticles</p>

“…9 LPNs combine advantages of both liposomes and polymers into a single platform, which is an ideal system for combinatorial delivery based on the dual-component structure. 10 pH-sensitive nanoparticles have been widely used to deliver drugs in cancer therapy due to the lower pH in tumors than in normal tissues. [11][12][13] In this study, pH-responsive LPNs were applied for the carboplatin and paclitaxel delivery.…”

Construction of Biocompatible Dual-Drug Loaded Complicated Nanoparticles for in vivo Improvement of Synergistic Chemotherapy in Esophageal Cancer

“…However, due to the difference in pharmacokinetics between the β-elemene and DOX, it is difficult to determine the optimal dose and drug ratio to exert optimal therapeutic effects at the tumor site. The combination of multiple drugs may produce better antitumor effects and the assessment of drug-drug interactions are critical (38). CI analysis is a common method for assessing the drugs interactions in combination therapy.…”

Utilization of Polymer-Lipid Hybrid Nanoparticles for Targeted Anti-Cancer Therapy

“…It is also reported that the ultra-small PLHNPs (25 nm) localized in a deep tissue region of the tumor with effective PTX delivery and excellent in vivo and in vitro stability in a mouse tumor model [ 53 ]. PLHNPs facilitate the delivery of numerous single chemotherapy drugs such as temozolomide, docetaxel, salinomycin, triptolide, paclitaxel, doxorubicin, and hydroxycamptothecin, among others specifically targeting different types of cancer [ 62 , 128 , 129 ]. Single drug-loaded PLHNPs have reduced in vivo and in vitro toxicity, good biocompatibility, excellent cellular internalization, and enhanced release kinetics [ 26 , 50 , 83 ].…”