Synergistic Effect of Barbadensis miller and Marsdenia Condurango Extracts Induces Apoptosis Promotes Oxidative Stress by Limiting Proliferation of Cervical Cancer and Liver Cancer Cells

Abstract

Background: Drug synergy is the combine effect of drug efficacy. Synergistic combinations of active ingredients have proven to be highly effective and more useful in therapeutics. In contrast, the individual effect of drug is usually undesirable and mostly used for selecting drug-resistant mutations. Purpose of this study was to check synergistic effects of both plants (Barbadensis miller and Marsdenia condurango) against liver and cervical cancer. Methodology: Culturing of HeLa (cervical cancer cell line) and HepG2 (liver cancer cell line) cells, IC 50 evaluation, viability assays (trypan blue, crystal violet), p53 ELISA and immunocytochemistry, MUSE analysis (count and viability), antioxidants (GSH, SOD, CAT), at the end RT-PCR was performed. Results: IC 50 evaluation was done of each plant individually and with combination for synergistic effects, IC 50 with plants combination (synergism) was applied on further viability assays (trypan blue, crystal violet, MUSE analysis via count and viability kit) p53 ELISA and immunocytochemistry for evaluation of cellular apoptosis, antioxidants assays (GSH, SOD, CAT), and RT-PCR with proliferative and apoptotic markers along with internal control. Conclusion: According to current study it was observed that synergistic effect of these plants has more anticancer properties with minimum effective dose. It was also observed that extracts possess the ability to induce apoptosis, restrict proliferation and enhanced oxidative stress.

Untitled

“…Furthermore, studies have shown that phytotherapeutic products rich in antioxidants might reduce the risk of cancer. As a result, there is a pressing need to identify novel anticancer drugs derived from plants with significant antioxidant activity [22]. The main goal of this investigation was to discover if Datura metel extracts had any apoptotic, anti-proliferative, and antioxidative activities against the HepG2 cell line in comparison to the normal BHK cell line.…”

In Vitro Study to Evaluate the Anticancer Potential of Chrysanthemum indicum, Therapeutic Plant

“…The majority of plant-derived anticancer medicines induce intrinsic or extrinsic apoptosis, as well as caspase and/or p53-dependent or independent processes (19). Annexin V staining reveals a substantial proportion of apoptosis after treatment with plant extracts in multiple studies (6,20). The plants' ethanol and hexane induces increased apoptotic activity in HepG2 cells through annexin V-dependent pathways, resulting in a more apoptotic activity in the cells.…”