Synergistic Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma in Streptozotocin-Induced Diabetic Rats

Abstract

BackgroundDiabetic wounds are a major clinical challenge, because minor skin wounds can lead to chronic, unhealed ulcers and ultimately result in infection, gangrene, or even amputation. Studies on bone marrow derived mesenchymal stem cells (BMSCs) and a series of growth factors have revealed their many benefits for wound healing and regeneration. Platelet-rich plasma (PRP) may improve the environment for BMSC development and differentiation. However, whether combined use of BMSCs and PRP may be more effective for accelerating diabetic ulcer healing remains unclear.ObjectiveWe investigated the efficacy of BMSCs and PRP for the repair of refractory wound healing in a diabetic rat model.MethodsForty-eight rats with diabetes mellitus induced by streptozotocin were divided into four groups: treatment with BMSCs plus PRP, BMSCs alone, PRP alone, phosphate buffered saline. The rate of wound closure was quantified. A histopathological study was conducted regarding wound depth and the skin edge at 7, 14, and 28 days after surgery.ResultsWound healing rates were significantly higher in the BMSC plus PRP group than in the other groups. The immunohistochemistry results showed that the expression of platelet/endothelial cell adhesion molecule 1, proliferating cell nuclear antigen, and transforming growth factor-β1 increased significantly in the BMSC plus PRP group compared to the other treatment groups. On day 7, CD68 expression increased significantly in the wounds of the BMSC plus PRP group, but decreased markedly at day 14 compared to the controls.ConclusionThe combination of BMSCs and PRP aids diabetic wound repair and regeneration.

Diabetes mellitus and cellular replacement therapy: Expected clinical potential and perspectives

“…It is expected that microenvironmental of hBM-MSCs may improve trans-differentiation this type of cell into insulinproduced β-cells. There are data that platelet-rich plasma might be useful for increasing of differentiation capacity of the hBM-MSCs [34] . Moreover, it has been postulated that hBM-MSCs probably would be considered more optimal candidates for further clinical implementation when compared with induced PSC, while this predisposition is required strong and continuous investigations.…”

Homogenous demineralized dentin matrix and platelet-rich plasma for bone tissue engineering in cranioplasty of diabetic rabbits: biochemical, radiographic, and histological analysis

“…These proteins regulate cell migration and proliferation (mitogenesis), remodel the extracellular matrix, and promote angiogenesis, creating a favourable environment that quickly stimulates tissue repair and wound healing. 13,14 Various studies on the complications of diabetes mellitus have demonstrated extensive alterations to bone and mineral metabolism, causing diabetic osteopenia. Some of the factors that have been associated with the pathogenesis of this bone disorder include hyperglycaemia, oxidative stress, and body weight loss.…”

Stem cells and chronic wound healing: state of the art

“…Increasing stem cell potency with adjuvants such as platelet-rich plasma or Panax notoginseng saponins are possible alternatives for decreasing the minimum required dose. 95,96 Means of prolonging transplanted cell lifespan are now heavily sought, as MSCs must survive to influence healing. Numerous possibilities have arisen, such as hyperoxic and pan-caspase pretreatment, which reduces MSC apoptosis in ischemic microenvironments.…”