Synergistic effect of methyljasmonate and cyclodextrin on stilbene biosynthesis pathway gene expression and resveratrol production in Monastrell grapevine cell cultures

Abstract

Background: Plant cell cultures have been shown as feasible systems for the production of secondary metabolites, being the elicitation with biotic or abiotic stimuli the most efficient strategy to increase the production of those metabolites. Vitaceae phytoalexins constitute a group of molecules belonging to the stilbene family which are derivatives of the trans-resveratrol structure and are produced by plants and cell cultures as a response to biotic and abiotic stresses. The potential benefits of resveratrol on human health have made it one of the most thoroughly studied phytochemical molecules. The aim of this study was to evaluate the elicitor effect of both cyclodextrin (CD) and methyljasmonate (MeJA) on grapevine cell cultures by carrying out a quantitative analysis of their role on resveratrol production and on the expression of stilbene biosynthetic genes in Vitis vinifera cv Monastrell albino cell suspension cultures.

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

“…Monastrell. These results correlate well with those obtained by Martinez-Esteso et al [58] and by Lijavetzky et al [22], respectively. …”

“…Our group determined that the elicitation treatments of cell suspensions V. vinifera cv.Gamay and cv. Monastrell with MBCD combined with MeJA led to major increases in t-R accumulation [22,58] because t-R was synthesised abundantly in treated cells and translocated continuously to the medium. However, treatment with MBCD+MeJA did not lead to the de novo synthesis of c-R, which was a minority metabolite compared to t-R. Figure 5 shows the amount of accumulated t-R in extracellular medium after 72 h of incubation with elicitors MBCD and MeJA, which were quantitatively determined by HPLC.…”

“…Experiments were done in duplicate. The spent medium was used to analyse t-R, as described elsewhere [22], by liquid chromatography in an Agilent 1100 series HPLC equipped with UV-vis and ESI-MS/MS ion trap detectors. For stilbenoid determination, 10 μl of the sample, after passing through an Anopore 0.2 μm filter, were injected into a Mediterranean C18 column (25 × 0.46 cm, 5 µm particle size) …”

“…Two major strategies can be carried out to overcome this drawback: elicitation and metabolic engineering. Elicitation is an empirical approach done to stimulate cell cultures to produce secondary metabolites; for instance, grapevine cell cultures elicited with methylated cyclodextrins (MBCD) alone [21] or combined with methyl jasmonate (MeJA) [22,23] has proven an efficient system for the bioproduction of t-R, with 30% overall conversion of feeding sucrose carbon into resveratrol carbon [24]. Metabolic engineering has been currently applied to microorganisms as an alternative way for the bioproduction of t-R and analogues [25][26][27][28] due to the obvious advantages over plant cell cultures to develop transformed strains and to upscale the productive process.…”

Silver nano fabrication using leaf disc of Passiflora foetida Linn

“…A passion flower has been reported as a symbol for Passion of Christ (Brindha et al 2012) and its attractive flower compromise of 100 petals that resemble as Kauravas, 5 stamens resemblance Pandavas, 3 stigmas as Brahma, Visnu and Shiva characters from historical Mahabharata in India (Lade 2015). These species usually grow in spiky trees as support and over the surface of alongside a road and lava flow, volcanic and limestone soils and is grown in waste land (Lijavetzky et al 2008). The minimum rainfall required for P. foetida is 900 mm.…”