Synergistic effect of upstream sequences, CCAAT box elements, and HSE sequences for enhanced expression of chimaeric heat shock genes in transgenic tobacco

Rieping, Mechthild; Schöffl, Fritz

doi:10.1007/bf00279795

Cited by 129 publications

(38 citation statements)

References 44 publications

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“…By searching the PLACE database with the 1‐kb upstream regions of the 29 rice CDPK genes as queries, we found that at least eight previously‐identified putative stress‐responsive cis ‐elements existed in the promoter regions of rice CDPK genes. These cis ‐elements included the ABA‐responsive EBOXBNNAPA [34], the light‐regulated IBOXCORE [35], the three dehydration‐responsive elements MYBCORE [36–38], MYCATRD22 [39,40] and DRECRTCOREATC [41–43], the cold‐responsive LTRECOREATCOR15 [44,45], the heat shock‐responsive CCAATBOX [46,47], and the defense‐responsive WBOXATNPR1 [48,49] (Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…This study has provided evidence that the upstream regions of all 29 CDPK genes contain multiple different stress‐responsive cis ‐elements including EBOXBNNAPA, MYBCORE, MYCATRD22, DRECRTCOREATC, LTRECOREATCOR15, CCAATBOX, IBOXCORE and WBOXATNPR1 (Table 3). These cis ‐elements play important roles in regulating expression of genes in response to ABA, dehydration, low temperature, heat shock and light and pathogen elicitors (Table 2) [34–49].…”

Section: Discussionmentioning

confidence: 99%

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Expression of rice Ca²⁺‐dependent protein kinases (CDPKs) genes under different environmental stresses

2007

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Ca 2+ -dependent protein kinases (CDPKs) play an essential role in plant Ca 2+ -mediated signal transduction. Twenty-nine CDPK genes have been identified in the rice genome through a complete search of genome and full-length cDNA databases. Eight of them were reported previously to be inducible by different stress stimuli. Sequence comparison revealed that all 29 CDPK genes (OsCPK1-29) contain multiple stress-responsive cis-elements in the promoter region (1 kb) upstream of genes. Analysis of the information extracted from the Rice Expression Database indicates that 11 of the CDPK genes are regulated by chilling temperature, dehydration, salt, rice blast infection and chitin treatment. RT-PCR and RNA gel blot hybridization were performed in this study to detect the expression 19 of the CDPK genes. Twelve CDPK genes exhibited cultivar-and tissue-specific expression; four CDPK genes (OsCPK6, OsCPK13, OsCPK17 and OsCPK25) were induced by chilling temperature, dehydration and salt stresses in the rice seedlings. While OsCPK13 (OsCDPK7) was already known to be inducible by chilling temperature and high salt, this is the first report that the other three genes are stress-regulated. OsCPK6 and OsCPK25 are up-regulated by dehydration and heat shock, respectively, while OsCPK17 is down-regulated by chilling temperature, dehydration and high salt stresses. Based on this evidence, rice CDPK genes may be important components in the signal transduction pathways for stress responses. Findings from this research are important for further dissecting mechanisms of stress response and functions of CDPK genes in rice.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of rice Ca²⁺‐dependent protein kinases (CDPKs) genes under different environmental stresses

2007

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“…It was shown that deletion of STRE motif from the promoter of Arabidopsis HSP90-1 gene decreases the promoter activity under heat stress conditions (Haralampidis et al 2002). Another heat shock-related element present in the promoter is CCAAT box, which has been found to act co-operatively with the HSEs to enhance HSP promoter activity during heat stress (Rieping & Schoffl 1992).…”

Section: Analysis Of Tahsp26 Promotermentioning

confidence: 99%

The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress

Chauhan

Khurana

Nijhavan

et al. 2012

Plant Cell & Environment

168

100

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The nuclear-encoded chloroplast small heat shock proteins (sHSPs) are present in all plant species from algae to angiosperms. Expression analysis shows that the wheat chloroplastic sHSP (HSP26) is highly inducible by heat stress in almost all the vegetative and generative tissues and is also expressed constitutively in certain developmental growth stages. We characterize wheat chloroplastic sHSP 26 through transgenic approach using Arabidopsis and report cloning of the promoter and its characterization. Transgenic Arabidopsis plants were substantially tolerant under continuous high temperature regimen than wild-type plants, as measured by photosystem II (PSII) activity, accumulation of more photosynthetic pigments, higher biomass and seed yield. Transgenic plants produced bold seeds under high temperature, having higher germination potential than the wild-type plants. Further, antisense Arabidopsis plants showed negligible tolerance even for non-lethal heat shock, impaired in basal thermo-tolerance, and accumulated less biomass and seed yield under normal growth conditions. Promoter analysis revealed the presence of several heat and other abiotic stress responsive cis-acting elements along with developmental stage and tissue-specific elements. Analysis of promoter through GUS reporter system in both transgenic rice and Arabidopsis further confirms the role of chloroplastic sHsp26 in heat and other abiotic stresses as well as during seed maturation and germination. Genome-wide expression analysis of overexpression Arabidopsis plants revealed that the transcriptome remained unchanged in the transgenic plants and the tolerance was due to the overexpression of chloroplastic heat shock protein (HSP) only.

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“…According to our in silico examination of transcriptional regulatory elements, the AtP3B promoter contains an HSE and several CCAATBOX1s. HSE is a cis-acting element that confers heat-stress responsiveness on promoters that contain it (Santos et al 1996), and CCAATBOX1 is also common in the promoters of heatshock genes (Rieping & Schoffl 1992).…”

Section: Discussionmentioning

confidence: 99%

Ribosomal P3 protein AtP3B of Arabidopsis acts as both protein and RNA chaperone to increase tolerance of heat and cold stresses

Kang

Lee

Park

et al. 2016

Plant Cell & Environment

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The P3 proteins are plant-specific ribosomal P-proteins; however, their molecular functions have not been characterized. In a screen for components of heat-stable high-molecular weight (HMW) complexes, we isolated the P3 protein AtP3B from heat-treated Arabidopsis suspension cultures. By size-exclusion chromatography (SEC), SDS-PAGE and native PAGE followed by immunoblotting with anti-AtP3B antibody, we showed that AtP3B was stably retained in HMW complexes following heat shock. The level of AtP3B mRNA increased in response to both high- and low-temperature stresses. Bacterially expressed recombinant AtP3B protein exhibited both protein and RNA chaperone activities. Knockdown of AtP3B by RNAi made plants sensitive to both high- and low-temperature stresses, whereas overexpression of AtP3B increased tolerance of both conditions. Together, our results suggest that AtP3B protects cells against both high- and low-temperature stresses. These findings provide novel insight into the molecular functions and in vivo roles of acidic ribosomal P-proteins, thereby expanding our knowledge of the protein production machinery.

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Synergistic effect of upstream sequences, CCAAT box elements, and HSE sequences for enhanced expression of chimaeric heat shock genes in transgenic tobacco

Cited by 129 publications

References 44 publications

Expression of rice Ca²⁺‐dependent protein kinases (CDPKs) genes under different environmental stresses

Expression of rice Ca²⁺‐dependent protein kinases (CDPKs) genes under different environmental stresses

The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress

Ribosomal P3 protein AtP3B of Arabidopsis acts as both protein and RNA chaperone to increase tolerance of heat and cold stresses

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Synergistic effect of upstream sequences, CCAAT box elements, and HSE sequences for enhanced expression of chimaeric heat shock genes in transgenic tobacco

Cited by 129 publications

References 44 publications

Expression of rice Ca2+‐dependent protein kinases (CDPKs) genes under different environmental stresses

Expression of rice Ca2+‐dependent protein kinases (CDPKs) genes under different environmental stresses

The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress

Ribosomal P3 protein AtP3B of Arabidopsis acts as both protein and RNA chaperone to increase tolerance of heat and cold stresses

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Resources

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Expression of rice Ca²⁺‐dependent protein kinases (CDPKs) genes under different environmental stresses

Expression of rice Ca²⁺‐dependent protein kinases (CDPKs) genes under different environmental stresses