Synergistic effects of dimethyloxalylglycine and butyrate incorporated into α-calcium sulfate on bone regeneration

Woo, Kyung Mi; Jung, Hyun-Kyo; Oh, Joung-Hwan; Rahman, Saeed Ur; Kim, Soung Min; Baek, Jeong‐Hwa; Ryoo, Hyun‐Mo

doi:10.1016/j.biomaterials.2014.10.054

Cited by 53 publications

(44 citation statements)

References 55 publications

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“…Indeed, Irwin et al [7] showed that DMOG increased adipogenic gene expression and decreased osteoblastic genes. On the other hand, Woo et al [8]reported that DMOG accelerated bone regeneration, which is supported by Peng et al [9] showing that DMOG prevented bone loss after ovariectomy.…”

Section: Introductionsupporting

confidence: 58%

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Dimethyloxalylglycine Promotes Bone Marrow Mesenchymal Stem Cell Osteogenesis via Rho/ROCK Signaling

Zhang

Jia

Zhao

et al. 2016

Cell Physiol Biochem

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Background/Aims: We investigated the role of dimethyloxalylglycine (DMOG) in bone marrow mesenchymal stem cell (BMSC) osteogenesis mediated by RhoA/ROCK. Methods: BMSCs were cultured with and without DMOG and/or Y-27632 (ROCK1 inhibitor). Cell proliferation, alkaline phosphatase (ALP) levels, and calcium deposits were determined. The expression of Runx2, OSX, p-cofilin, RhoA, and GTP-bound RhoA was determined by real-time RT-PCR and Western blot. Rho-associated coiled-coil-containing protein kinase (ROCK) activity was determined by measuring the phosphorylation of myosin-binding subunit of myosin phosphatase using an ELISA kit. Actin morphology was observed by immunofluorescence. Results: After 24 h, DMOG (0.5 mM) increased the expression of GTP-bound RhoA (+141%, P < 0.001) and enhanced ROCK activity (315%, P < 0.001). DMOG (0.5 mM) enhanced ALP levels after 3, 7, and 21 days of osteogenic induction (all P < 0.001) and strengthened calcium deposition (P < 0.001). In addition, compared with controls, DMOG (0.5 mM) increased the mRNA levels of osteogenesis genes RUNX2 and OSX (all P < 0.001). Furthermore, compared with controls, DMOG increased the expression of p-cofilin (+57%, P < 0.001), which resulted in rearrangement of actin filaments. All these effects were abolished, at least in part, by Y-27632. Conclusion: DMOG promotes BMSC osteogenic differentiation via activation of RhoA/ROCK, suggesting clues for future therapies using BMSCs.

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Section: Introductionsupporting

confidence: 58%

“…Similarly, Irwin et al [7]found that 5 mM DMOG inhibited BMSCs osteogenic differentiation and their adipogenic differentiation. Woo et al [8]showed that DMOG promoted osteogenesis and bone regeneration in vivo. Peng et al [9] indicated that DMOG prevented bone loss in ovariectomized C57BL/6J mice via enhanced angiogenesis and osteogenesis.…”

Section: Discussionmentioning

confidence: 99%

Dimethyloxalylglycine Promotes Bone Marrow Mesenchymal Stem Cell Osteogenesis via Rho/ROCK Signaling

Zhang

Jia

Zhao

et al. 2016

Cell Physiol Biochem

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“…Moreover, butyrate incorporated into small molecules, e.g. α-calcium sulfate, improves osteoblast differentiation and pro-angiogenic responses, and in consequence stimulates bone regeneration (36).…”

Section: Discussionmentioning

confidence: 99%

“…Under these conditions of intensive methods of production and stress much greater strength and effectiveness of the bone development are absolutely essential. Taking into account that sodium butyrate positively influences nutrients digestion, absorption area, and integrity of the small intestine (5, 10), stimulates osteoblast differentiation, and promotes bone regeneration (36); thus it can be assumed that dietary sodium butyrate supplementation may be helpful in preventing problems of growth and mineralisation of bones in post-weaning period. However, one should also take into consideration the fact that butyrate influences osteoclast formation and differentiation (3), and in this way it can stimulate osteoclastogenesis and osteoclast activity.…”

Section: Introductionmentioning

confidence: 99%

Effect of sodium butyrate and Yucca schidigera extract on bone characteristics in growing pigs

Puzio¹,

Piedra

Kapica³

et al. 2016

Journal of Veterinary Research

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Introduction: The aim of this study was to investigate the influence of diet supplementation with sodium butyrate and Yucca schidigera extract (0.2% and 0.3%) on femur quality of growing pigs (n = 45). Material and Methods: At the age of 28, 35 and 56 d, five piglets from each group fed a different diet were euthanised and the femora were collected for further analyses. The bone characteristics were assessed based on weight, length, densitometric analysis of BMC and BMD, pQCT analysis (area, mineral content, volumetric density of trabecular and cortical part of metaphysis and diaphysis, respectively), ultimate strength, and geometrical parameters (cross-sectional area and second moment of inertia). Results: There were no significant differences in femur bone parameters among experimental groups on the 28 th d of life. On the 35 th d of life, piglets with 0.2% supplementation of sodium butyrate and Yucca schidigera extract had significantly lower values of weight and second moment of inertia, and significantly higher trabecular BMD and BMC compared to other experimental groups. In 56-day-old pigs, the higher values were observed in both experimental groups regarding BMC, ultimate strength, geometrical parameters, cortical BMC, diaphyseal total area, and endosteal circumference (P < 0.05). Significant differences between experimental groups were observed only in bone weight and cortical thickness. Conclusion: This study proved that simultaneous supplementation with sodium butyrate and Yucca schidigera extract positively influences bone quality in pigs in the post-weaning period. However, there were no differences in bone characteristics between the addition of 0.2% and 0.3% preparations.

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“…Previous studies have shown that small molecules and biomaterial scaffolds can induce the osteoblast differentiation and bone regeneration [215]. These studies have shown that markers for the osteoblast differentiation were increased in cells cultured on scaffold substrates.…”

Section: Applications Of Materials For Osteoblast Differentiation mentioning

confidence: 99%

Nanoscale and Macroscale Scaffolds with Controlled-Release Polymeric Systems for Dental Craniomaxillofacial Tissue Engineering

et al. 2018

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Tremendous progress in stem cell biology has resulted in a major current focus on effective modalities to promote directed cellular behavior for clinical therapy. The fundamental principles of tissue engineering are aimed at providing soluble and insoluble biological cues to promote these directed biological responses. Better understanding of extracellular matrix functions is ensuring optimal adhesive substrates to promote cell mobility and a suitable physical niche to direct stem cell responses. Further, appreciation of the roles of matrix constituents as morphogen cues, termed matrikines or matricryptins, are also now being directly exploited in biomaterial design. These insoluble topological cues can be presented at both micro- and nanoscales with specific fabrication techniques. Progress in development and molecular biology has described key roles for a range of biological molecules, such as proteins, lipids, and nucleic acids, to serve as morphogens promoting directed behavior in stem cells. Controlled-release systems involving encapsulation of bioactive agents within polymeric carriers are enabling utilization of soluble cues. Using our efforts at dental craniofacial tissue engineering, this narrative review focuses on outlining specific biomaterial fabrication techniques, such as electrospinning, gas foaming, and 3D printing used in combination with polymeric nano- or microspheres. These avenues are providing unprecedented therapeutic opportunities for precision bioengineering for regenerative applications.

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Synergistic effects of dimethyloxalylglycine and butyrate incorporated into α-calcium sulfate on bone regeneration

Cited by 53 publications

References 55 publications

Dimethyloxalylglycine Promotes Bone Marrow Mesenchymal Stem Cell Osteogenesis via Rho/ROCK Signaling

Dimethyloxalylglycine Promotes Bone Marrow Mesenchymal Stem Cell Osteogenesis via Rho/ROCK Signaling

Effect of sodium butyrate and Yucca schidigera extract on bone characteristics in growing pigs

Nanoscale and Macroscale Scaffolds with Controlled-Release Polymeric Systems for Dental Craniomaxillofacial Tissue Engineering

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