Synergistic effects of hyaluronate – epidermal growth factor conjugate patch on chronic wound healing

Kim, Yun Seop; Sung, Dong Kyung; Kong, Won Ho; Kim, Hye Min; Hahn, Sei Kwang

doi:10.1039/c8bm00079d

Cited by 28 publications

(21 citation statements)

References 29 publications

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“…Exogenous EGF is easily degraded in a chronic wound environment, which limits its application in the process of chronic wound healing [24]. Kim et al [42] found that a hyaluronate-EGF conjugate patch plays an important role in chronic wound healing. Meanwhile, the application of new nano-biomaterials has greatly promoted the development in the field of wound healing [43].…”

Section: Discussionmentioning

confidence: 99%

Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Wei

Wang

et al. 2021

Military Med Res

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Background Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore the effects of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF. Methods We tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations (0, 1, 5, 10, and 100 ng/ml) and different EGF action times (2, 10, and 30 min). A full-thickness skin defect model was established using male, 30-week-old Bama pigs. The experiment included groups as follows: routine dressing change after covering with sterile auxiliary material (Control), continuous negative pressure drainage of the wound (VSD), continuous negative pressure drainage of the wound and injection of EGF 10 min followed by removal by continuous lavage (V + E 10 min), and continuous negative pressure drainage of the wound and injection of EGF 30 min followed by removal by continuous lavage (V + E 30 min). The wound healing rate, histological repair effect and collagen deposition were compared among the four groups. Results An EGF concentration of 10 ng/ml and an action time of 10 min had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells. The drug dispersion effect was better than drug infusion after bolus injection effect, and the contact surface was wider. Compared with other groups, the V + E 10 min group promoted wound healing to the greatest extent and obtained the best histological score. Conclusions A recombinant human epidermal growth factor (rhEGF) concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent in vitro. VSD combined with rhEGF kept in place for 10 min and then washed, can promote wound healing better than the other treatments in vivo.

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Section: Discussionmentioning

confidence: 99%

Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Wei

Wang

et al. 2021

Military Med Res

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“…However, some cells within wounds lack growth factor stimuli due to defects in molecular components (e.g., downregulation of receptor). 8,50,51 Stem cell-based therapies.…”

Section: Current Regenerative Therapies and Their Limitationmentioning

confidence: 99%

Approaches for Regenerative Healing of Cutaneous Wound with an Emphasis on Strategies Activating the Wnt/β-Catenin Pathway

Choi

Yoon

Choi

2022

Advances in Wound Care

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In adult mammals, spontaneous repair of a cutaneous wound occurs slowly and leaves a scar with skin adnexa deficiencies. To accelerate cutaneous wound-healing rates and avoid scar formation, current studies have focused on regenerative therapies. Recent Advances: Emerging therapeutics for regenerative wound healing often focus on the use of growth factors and stem cells. However, these therapeutic approaches have limited routine clinical use due to high costs and technical requirements. Critical Issue: Understanding the molecular mechanisms involved in the signaling pathways for cutaneous wound healing and neogenic synthesis of the skin components is important for identification of novel targets for the development of regenerative wound-healing agents. Future Directions: The Wnt/b-catenin pathway is a well-known key player for enhancement of the overall healing process involving tissue regeneration via crosstalk with other signaling pathways. Strategies that activate the Wnt/bcatenin pathway via modulation of the pathway-controlling regulatory factors could provide effective therapeutic approaches for regenerative wound healing.

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“…Exogenous EGF is easily degraded in a chronic wound environment, which limits its application in the process of chronic wound healing [23]. Kim et al [41] found that a hyaluronate −epidermal growth factor conjugate patch plays an important role in chronic wound healing. Meanwhile, the application of new nano-biomaterials has greatly promoted the development in the eld of wound healing [42].…”

Section: Discussionmentioning

confidence: 99%

Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Wei

Wang

et al. 2020

Preprint

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Background: Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore the effect of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF.Methods: In this study, we tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations (0, 1, 5, 10, and 100 ng/ml) and different EGF action times (2, 10, and 30min). A full-thickness skin defect model was established using male, 30-week-old Bama pigs. The skin defects were randomly divided follows: routine dressing change after covering with sterile auxiliary material (Control), continuous negative pressure drainage of the wound (VSD), continuous negative pressure drainage of the wound and injection of epidermal growth factor 10 minutes followed by removal by continuous lavage (V+E 10 min), and continuous negative pressure drainage of the wound and injection of epidermal growth factor 30 minutes followed by removal by continuous lavage (V+E 30 min). The wound healing rate, histological repair effect and collagen deposition were compared among the four groups.Results: An EGF concentration of 10 ng/ml and an action time of 10 minutes had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells. The drug dispersion effect was better than drug infusion after bolus injection effect, and the contact surface was wider. Compared with other groups, the V+E 10min group promoted wound healing to the greatest extent and obtained the best histological score. Conclusions: A rhEGF concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent in vitro. VSD combined with rhEGF kept in place for 10 minutes and then washed, can promote wound healing better than the other treatments in vivo.

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Synergistic effects of hyaluronate – epidermal growth factor conjugate patch on chronic wound healing

Cited by 28 publications

References 29 publications

Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Approaches for Regenerative Healing of Cutaneous Wound with an Emphasis on Strategies Activating the Wnt/β-Catenin Pathway

Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

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