Synergistic effects of interferon γ and tumour necrosis factor α on T84 cell function

Abstract

(Gut 1999;45:191-198)

Proinflammatory cytokines TNF-α and IFN-γ alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells

“…A similar synergy has also been reported in various intestinal epithelial cell models for specific chemokine secretion (58), inhibition of cell growth (47), alteration in epithelial barrier properties (21), and acquisition of susceptibility to Fas-induced apoptosis (46). The pleiotropic effects of the TNF-␣/IFN-␥ combination on epithelial cells are thus likely to be of functional relevance to the inflammatory bowel disease pathogenesis.…”

14-3-3 Proteins regulate Akt Thr308 phosphorylation in intestinal epithelial cells

“…Figure 3D). 1,[36][37][38] Akt1 and 14-3-3ζ have an active role in this process. 1,2 Therefore, we investigated the effect of inhibiting 14-3-3 function in Akt1 signaling using 14-3-3ζ regulates Akt activation M Gómez-Suárez et al the DSS-colitis model.…”

Irinotecan disrupts tight junction proteins within the gut

“…able to modulate tight junction proteins 26,39 and it is therefore hypothesized that cytokine-mediated tight junction modulation may play a key role in the development of CIGT. 10 In vitro application of tumor necrosis factor (TNF) has been shown to induce actin and tight junction rearrangement, resulting in a time-dependent decrease in tight junction protein expression and a parallel increase in intestinal permeability.…”

“…Importantly, proinflammatory cytokines 26 and pathogenic bacteria 27 exhibit modulatory effects on tight junction proteins and therefore may be responsible for changes in barrier integrity. Breakdown of the mucosal barrier enables noxious agents and pathogens to penetrate the epithelium.…”