Synergistic effects of plant growth-promoting Neorhizobium huautlense T1-17 and immobilizers on the growth and heavy metal accumulation of edible tissues of hot pepper

Chen, Ling; He, Linyan; Wang, Qi; Sheng, Xiafang

doi:10.1016/j.jhazmat.2016.03.042

Cited by 77 publications

(26 citation statements)

References 39 publications

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“…In many instances, biofilms formed on the surfaces of plant roots are beneficial to their hosts, for example, by preventing the growth of bacterial and fungal pathogens (7)(8)(9), and thus can be used as biocontrol agents. In addition, soil microbiota community members, such as Bacillus subtilis, belong to the group of plant growth-promoting rhizobacteria (PGPR) which, when applied to soils, can stimulate plant growth and increase yields (10)(11)(12)(13). In addition to beneficial interactions with plants, bacterial biofilms also have an active role in the bioremediation of contaminated soils (14) and in carbon dioxide sequestration (15) and thus are of enormous ecological importance.…”

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confidence: 99%

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

Ogran

Yardeni

Keren-Paz

et al. 2019

Appl Environ Microbiol

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Microbial ecosystems tightly associated with a eukaryotic host are widespread in nature. The genetic and metabolic networks of the eukaryotic hosts and the associated microbes have coevolved to form a symbiotic relationship. Both the Gram-positive Bacillus subtilis and the Gram-negative Serratia plymuthica can form biofilms on plant roots and thus can serve as a model system for the study of interspecies interactions in a host-associated ecosystem. We found that B. subtilis biofilms expand collectively and asymmetrically toward S. plymuthica, while expressing a nonribosomal antibiotic bacillaene and an extracellular protease. As a result, B. subtilis biofilms outcompeted S. plymuthica for successful colonization of the host. Strikingly, the plant host was able to enhance the efficiency of this killing by inducing bacillaene synthesis. In turn, B. subtilis biofilms increased the resistance of the plant host to pathogens. These results provide an example of how plant-bacterium symbiosis promotes the immune response of the plant host and the fitness of the associated bacteria. IMPORTANCE Our study sheds mechanistic light on how multicellular biofilm units compete to successfully colonize a eukaryote host, using B. subtilis microbial communities as our lens. The microbiota and its interactions with its host play various roles in the development and prevention of diseases. Using competing beneficial biofilms that are essential microbiota members on the plant host, we found that B. subtilis biofilms activate collective migration to capture their prey, followed by nonribosomal antibiotic synthesis. Plant hosts increase the efficiency of antibiotic production by B. subtilis biofilms, as they activate the synthesis of polyketides; therefore, our study provides evidence of a mechanism by which the host can indirectly select for beneficial microbiota members.on July 10, 2020 by guest http://aem.asm.org/ Downloaded from RESULTSB. subtilis biofilm actively expands toward a competing S. plymuthica colony. When grown on a solid biofilm-inducing medium, B. sutbilis biofilms form symmetrical, circular colonies. To determine the effect of a competing S. plymuthica colony on the development of a B. subtilis biofilm, the two species were inoculated next to each other on a solid biofilm medium. After 2 days, the B. subtilis colony reached S. plymuthica, forming a thick wrinkle around its edge and penetrating toward its center. By the third day, B. subtilis biofilm completely engulfed the S. plymuthica colony, covering it with a thin, unstructured film and enclosing it within the circular wrinkle (Fig. 1A). The location and shape of the B. subtilis center of the biofilm did not change during the interaction with S. plymuthica. However, the biofilm colony advanced asymmetrically toward the S. plymuthica colony, breaking from its usual circular shape ( Fig. 1A).We next examined the mechanisms that could mediate this asymmetric expansion toward a competitor. Many bacterial cells are capable of directional swimming using their flagella (2...

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mentioning

confidence: 99%

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

Ogran

Yardeni

Keren-Paz

et al. 2019

Appl Environ Microbiol

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“…In our work, the symbiotic gene nifH of Sinorhizobium strain YH1 was clustered nearer to Neorhizobium huautlense (formerly known as Rhizobium huautlense ) CCBAU 65798 with 99.6% homogeny than to Sinorhizobium saheli which only had 97.1% similarity. This could be explained by the fact that N. huautlense was also found to be a PGPR microbe that could reduce the accumulation of Cd by plant (Chen et al, 2016). In addition, a horizontal gene transfer could help further explain this phenomenon.…”

Section: Discussionmentioning

confidence: 99%

Inoculation of Sinorhizobium saheli YH1 Leads to Reduced Metal Uptake for Leucaena leucocephala Grown in Mine Tailings and Metal-Polluted Soils

Kang

Cui

et al. 2018

Front. Microbiol.

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Metalliferous mine tailings have a negative impact on the soil environment near mining areas and render cultivable lands infertile. Phytoremediation involving the synergism of legume and rhizobia provides a useful technique in tackling this issue with cost-effective, environmentally friendly, and easy-to-use features under adverse soil conditions. Leucaena leucocephala has been found to build symbiotic relationships with native rhizobia in the iron-vanadium-titanium oxide (V-Ti magnetite) mine tailing soil. Rhizobia YH1, isolated from the root nodules of L. leucocephala, was classified as Sinorhizobium saheli according to similarity and phylogenetic analyses of 16S rRNA, housekeeping and nitrogen fixation genes. Besides nitrogen fixation, S. saheli YH1 also showed capabilities to produce indole-acetic acid (IAA) (166.77 ± 2.03 mg l−1) and solubilize phosphate (104.41 ± 7.48 mg l−1). Pot culture experiments showed that strain YH1 increased the biomass, plant height and root length of L. leucocephala by 67.2, 39.5 and 27.2% respectively. There was also an average increase in plant N (10.0%), P (112.2%) and K (25.0%) contents compared to inoculation-free control. The inoculation of YH1 not only reduced the uptake of all metals by L. leucocephala in the mine tailings, but also resulted in decreased uptake of Cd by up to 79.9% and Mn by up to 67.6% for plants grown in soils contaminated with Cd/Mn. It was concluded that S. saheli YH1 possessed multiple beneficial effects on L. leucocephala grown in metalliferous soils. Our findings highlight the role of S. saheli YH1 in improving plant health of L. leucocephala by reducing metal uptake by plants grown in heavy metal-polluted soils. We also suggest the idea of using L. leucocephala-S. saheli association for phytoremediation and revegetation of V-Ti mine tailings and soils polluted with Cd or Mn.

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“…Diethylenetriaminepentaacetic acid (DTPA) extract solution was added to the soil sample at the ratio of 2.5:1 (solution: soil) for 4 h, then the supernatant solution was digested by electrothermal digestion. The contents of DTPA-extractable Cd and Pb in the extract solution were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) (SHIMADZ ICPE-9820, Japan) [30]. investigated.…”

Section: Sample Collection and Analysismentioning

confidence: 99%

“…Then, the dried water spinach roots and shoots were ground with a grinder, and 0.5 g was used for microwave digestion. The contents of Pb 2+ and Cd 2+ in the digestion solution were determined by ICP-AES [30].…”

Section: Analysis Of Samplesmentioning

confidence: 99%

Screening of Heavy Metal-Immobilizing Bacteria and Its Effect on Reducing Cd2+ and Pb2+ Concentrations in Water Spinach (Ipomoea aquatic Forsk.)

Wang

Tian

et al. 2020

IJERPH

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Microbial immobilization is considered as a novel and environmentally friendly technology that uses microbes to reduce heavy metals accumulation in plants. To explore microbial resources which are useful in these applications, three water spinach rhizosphere soils polluted by different levels of heavy metals (heavy pollution (CQ), medium pollution (JZ), and relative clean (NF)) were collected. The community composition of heavy metal-immobilizing bacteria in rhizosphere soils and its effects on reducing the Cd2+ and Pb2+ concentrations in water spinach were evaluated. Four hundred strains were isolated from the CQ (belonging to 3 phyla and 14 genera), JZ (belonging to 4 phyla and 25 genera) and NF (belonged to 6 phyla and 34 genera) samples, respectively. In the CQ sample, 137 strains showed a strong ability to immobilize Cd2+ and Pb2+, giving Cd2+ and Pb2+ removal rates of greater than 80% in solution; Brevundimonas, Serratia, and Pseudoarthrobacter were the main genera. In total, 62 strains showed a strong ability to immobilize Cd2+ and Pb2+ in the JZ sample and Bacillus and Serratia were the main genera. A total of 22 strains showed a strong ability to immobilize Cd2+ and Pb2+ in the NF sample, and Bacillus was the main genus. Compared to the control, Enterobacter bugandensis CQ-7, Bacillus thuringensis CQ-33, and Klebsiella michiganensis CQ-169 significantly increased the dry weight (17.16–148%) of water spinach and reduced the contents of Cd2+ (59.78–72.41%) and Pb2+ (43.36–74.21%) in water spinach. Moreover, the soluble protein and Vc contents in the shoots of water spinach were also significantly increased (72.1–193%) in the presence of strains CQ-7, CQ-33 and CQ-169 compared to the control. In addition, the contents of Cd and Pb in the shoots of water spinach meet the standard for limit of Cd2+ and Pb2+ in vegetables in the presence of strains CQ-7, CQ-33 and CQ-169. Thus, the results provide strains as resources and a theoretical basis for the remediation of Cd- and Pb-contaminated farmlands for the safe production of vegetables.

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Synergistic effects of plant growth-promoting Neorhizobium huautlense T1-17 and immobilizers on the growth and heavy metal accumulation of edible tissues of hot pepper

Cited by 77 publications

References 39 publications

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

Inoculation of Sinorhizobium saheli YH1 Leads to Reduced Metal Uptake for Leucaena leucocephala Grown in Mine Tailings and Metal-Polluted Soils

Screening of Heavy Metal-Immobilizing Bacteria and Its Effect on Reducing Cd2+ and Pb2+ Concentrations in Water Spinach (Ipomoea aquatic Forsk.)

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