Synergistic effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells

Abstract

OBJECTIVE: The effects of quercetin and selenium on oxidative stress in endometrial adenocarcinoma cells are unclear. In this study, the effects of quercetin and selenium on oxidative stress caused by both hydrogen peroxide and UV radiation in endometrial adenocarcinoma cells were examined. METHODS: The viability of endometrial adenocarcinoma cells cultured in vitro and treated with different concentrations of quercetin and sodium selenite was measured using the MTT assay. Malondialdehyde (MDA) levels were investigated, and expression levels of BAD and p53 genes were analysed using real-time quantitative polymerase chain reaction. Acridine orange/ethidium bromide staining technique was applied to detect apoptosis. Mass attenuation coeffi cient of each quercetin and sodium selenite combinations was evaluated using Monte Carlo simulation. RESULTS: The combination of quercetin and sodium selenite enhanced cell viability, and reduced MDA levels. The expression levels of BAD and p53 genes decreased by combined treatment with quercetin and selenium while showing synergistic effects in terms of gene expression. Fluorescent microscopic examination showed a decrease in apoptotic cells in endometrial adenocarcinoma cells treated with the combination of quercetin and selenium. CONCLUSIONS: For the fi rst time, selenium and quercetin have synergistic cytoprotective and radioprotective effects on oxidative stress caused by hydrogen peroxide in endometrial adenocarcinoma cells for the fi rst time

Investigation of Protective Effects of Quercetin on Oxidative Stress Induced by Vinblastine in Bone Marrow of Rats

“…MDA, as an end-product of lipid peroxidation, may be used as an indicator of oxidative stress that increases in the body after administration of vinblastine. Results also showed that the use of quercetin compound reduced malondialdehyde levels in the bone marrow compared to the group receiving vinblastine, which was similar to the results of previous studies, 34,35 showing that quercetin partially protects blood glutathione and leads to suppression of plasma malondialdehyde levels, as well as nitric oxide metabolites and the production of superoxide anion. Collectively, the results of the present study showed that chronic administration of quercetin in rats caused inhibition of lipid peroxidation.…”

Quercetin Protects against Lipopolysaccharide-Induced Intestinal Oxidative Stress in Broiler Chickens through Activation of Nrf2 Pathway

“…A previous study showed that quercetin could inhibit human intestinal oxidative stress by reducing ROS production [23]. Quercetin and selenium reduced MDA levels and inhibited oxidative stress caused by hydrogen peroxide and UV radiation in endometrial adenocarcinoma cells [24]. In the liver mitochondria, quercetin treatment decreased the ROS and MDA levels and increased the CAT and glutathione (GSH) levels [25].…”

Anti-cancer properties of quercetin in osteosarcoma

“…For instance, quercetin is able to protect cells against oxidative stress by decreasing the number of reactive oxygen species (ROS) [ 70 ]. Subsequently, signaling pathways induced by ROS which are participating in cancer initiation/progression are inhibited by quercetin [ 70 , 73 ]. In this regard, a great body of research has examined quercetin on different types of cancer.…”