Synergistic effects of reduced graphene oxide and hydroxyapatite on osteogenic differentiation of MC3T3-E1 preosteoblasts

Shin, Yong Cheol; Lee, Jong Ho; Jin, Oh Seong; Kang, Sang Hoon; Hong, Suck Won; Kim, Bongju; Park, Jong Chul; Han, Dong‐Wook

doi:10.1016/j.carbon.2015.09.028

Cited by 70 publications

(40 citation statements)

References 66 publications

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“…Due to the pressure applied at high temperatures, the morphology of the powders had changed slightly. Due to the coating of rGO sheets with HA and the proper bonding of the HA particles in the sintering process, it was expected that the twophase bonding (interface) was performed well [34,39]. Figure 4 shows the XRD and XPS analyzes of the HA-rGO nanocomposite.…”

Section: Preparation Of Ha-rgo Nanocompositementioning

confidence: 99%

Investigating the mechanical behavior of hydroxyapatite-reduced graphene oxide nanocomposite under different loading rates

Nosrati

Sarraf-Mamoory

et al. 2020

Nano Ex.

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In this study, the hydroxyapatite (HA)-reduced graphene oxide (rGO) nanocomposite was investigated for its mechanical properties. The nanocomposite used in this study was made in two stages. The HA-rGO powders were first synthesized by hydrogen gas injected hydrothermal method, and then consolidated by spark plasma sintering. HA-rGO nanocomposite was subjected to Vickers indentation experiments with different loading rates. Various analyzes have been used in this study, including x-rays diffraction, field emission scanning electron microscopy, high-resolution transmission electron microscopy, fast fourier transform, and inverse fast fourier transform. The findings of this study showed that the HA in this nanocomposite was reinforced with rGO sheets coated with HA. As the loading rate increased, the slope of the curves in the elastic region was increased, indicating that the elastic modulus was increased. Also, the contact depth at higher loading rates was increased. Plastic deformation was higher at higher loading rates and the hardness had increased. As the loading rate increased from 300 mN to 1 N, the hardness and elastic modulus increased with more slope than when the loading rate changed from 1 N to 2 N. The presence of rGO sheets had partially controlled the HA brittleness.

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Section: Preparation Of Ha-rgo Nanocompositementioning

confidence: 99%

Investigating the mechanical behavior of hydroxyapatite-reduced graphene oxide nanocomposite under different loading rates

Nosrati

Sarraf-Mamoory

et al. 2020

Nano Ex.

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“…The rGO sheets were obtained by the preparation of GO using the modified Hummers and offeman method, and subsequent reduction process of GO with hydrazine hydrate (N 2 H 4 ·H 2 O) at 100 • C for 24 h [38,49,50]. The prepared rGO sheets in deionized water were sonicated for 2 h. The DCP microparticles dispersed in deionized water were physically mixed at DCP to rGO weight ratios of 2:1, 1:1, or 1:2, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Meanwhile, the intrinsic brittleness, low wear resistance, and low fracture toughness of DCP are often considered as disadvantages [37,38].…”

Section: −mentioning

confidence: 99%

Dicalcium Phosphate Coated with Graphene Synergistically Increases Osteogenic Differentiation In Vitro

et al. 2017

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Abstract:In recent years, graphene and its derivatives have attracted much interest in various fields, including biomedical applications. In particular, increasing attention has been paid to the effects of reduced graphene oxide (rGO) on cellular behaviors. On the other hand, dicalcium phosphate (DCP) has been widely used in dental and pharmaceutical fields. In this study, DCP composites coated with rGO (DCP-rGO composites) were prepared at various concentration ratios (DCP to rGO concentration ratios of 5:2.5, 5:5, and 5:10 µg/mL, respectively), and their physicochemical properties were characterized. In addition, the effects of DCP-rGO hybrid composites on MC3T3-E1 preosteoblasts were investigated. It was found that the DCP-rGO composites had an irregular granule-like structure with a diameter in the range order of the micrometer, and were found to be partially covered and interconnected with a network of rGO. The zeta potential analysis showed that although both DCP microparticles and rGO sheets had negative surface charge, the DCP-rGO composites could be successfully formed by the unique structural properties of rGO. In addition, it was demonstrated that the DCP-rGO composites significantly increased alkaline phosphatase activity and extracellular calcium deposition, indicating that the DCP-rGO hybrid composites can accelerate the osteogenic differentiation by the synergistic effects of rGO and DCP. Therefore, in conclusion, it is suggested that the DCP-rGO hybrid composites can be potent factors in accelerating the bone tissue regeneration.

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“…These particles (from nanosized to microsized range) act either as nucleation centres for facilitating HA deposition on constructs or by releasing ions that induced stem cell differentiation, a process commonly termed as osteoinduction (Deng et al, ; Gao et al, ; Kim, Yun, & Kim, ; Sithole et al, ; Wenz, Borchers, Tovar, & Kluger, ; Zhai et al, ). Graphene is another material gaining momentum in bone regenerative studies (Hermenean et al, ; Lee et al, ; Lu et al, ; Shadjou & Hasanzadeh, ; Shin et al, ); however, its applicability to 3D bioprinted constructs is not fully explored yet (Wang et al, ; Sayyar, Officer, & Wallace, ; Zhou et al, ). Hence, bioink design forms a critical part of 3D bioprinted bone constructs, with advanced bioprinter designs, fabrication strategy, image conversion softwares, cell source, and seeding modalities, being other contributing factors.…”

Section: Introductionmentioning

confidence: 99%

Advances in three‐dimensional bioprinting of bone: Progress and challenges

Midha

Dalela

Sybil

et al. 2019

J Tissue Eng Regen Med

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Several attempts have been made to engineer a viable three‐dimensional (3D) bone tissue equivalent using conventional tissue engineering strategies, but with limited clinical success. Using 3D bioprinting technology, scientists have developed functional prototypes of clinically relevant and mechanically robust bone with a functional bone marrow. Although the field is in its infancy, it has shown immense potential in the field of bone tissue engineering by re‐establishing the 3D dynamic micro‐environment of the native bone. Inspite of their in vitro success, maintaining the viability and differentiation potential of such cell‐laden constructs overtime, and their subsequent preclinical testing in terms of stability, mechanical loading, immune responses, and osseointegrative potential still needs to be explored. Progress is slow due to several challenges such as but not limited to the choice of ink used for cell encapsulation, optimal cell source, bioprinting method suitable for replicating the heterogeneous tissues and organs, and so on. Here, we summarize the recent advancements in bioprinting of bone, their limitations, challenges, and strategies for future improvisations. The generated knowledge will provide deep insights on our current understanding of the cellular interactions with the hydrogel matrices and help to unravel new methodologies for facilitating precisely regulated stem cell behaviour.

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Synergistic effects of reduced graphene oxide and hydroxyapatite on osteogenic differentiation of MC3T3-E1 preosteoblasts

Cited by 70 publications

References 66 publications

Investigating the mechanical behavior of hydroxyapatite-reduced graphene oxide nanocomposite under different loading rates

Investigating the mechanical behavior of hydroxyapatite-reduced graphene oxide nanocomposite under different loading rates

Dicalcium Phosphate Coated with Graphene Synergistically Increases Osteogenic Differentiation In Vitro

Advances in three‐dimensional bioprinting of bone: Progress and challenges

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