Synergistic effects of some metals contaminating mussels on the cytotoxicity of the marine toxin okadaic acid

Traoré, Adama; Bonini, M.; Dano, Sébastien D.; Creppy, Edmond E.

doi:10.1007/s002040050620

Cited by 26 publications

(21 citation statements)

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“…Contamination of mussels, seafood, and bivalves by toxic metals is frequently reported worldwide these last years in the range of 0.04-4.9 lg/g for Cd, 0.1-0.4 lg/g for Pb, 8-235 lg/g for Cu, and 0.15-0.75 lg/g for Cr (Hopenhayn-Rich et al, 1998;Saavedra et al, 2004;Bebianno et al, 2004;Romeo et al, 2006). Concentrations of heavy metal detected here are clearly higher than the one detected in mussels from the south part of France this last decade for Cd, that is 0.83 lg/g dried weight versus 1.08 lg/g of wet flesh weight, corresponding approximately to 5.4 lg/g of dried weight (Traoré et al, 1999). The recent application by the European Community of a 1 lg Cd/g (wet weight) imposed limit to bivalve molluscs and the current deliberation by CODEX to adopt the same value (for a review see Kruzynski, 2004), pose significant threats to the shellfish export trade from Tunisia.…”

Section: Discussionmentioning

confidence: 46%

“…Thus it is not surprising that in bivalves contaminated by toxic metals ions (Saavedra et al, 2004;Bocio et al, 2005), marine toxins are also found (Traoré et al, 1999(Traoré et al, , 2000Vale and de M Sampayo, 2001). The seafood production area of Boughrara, Tunisia, was banned a year ago because of pollution by heavy metals.…”

Section: Introductionmentioning

confidence: 99%

“…Okadaic acid (OA) has similar targets in cells. Their combinations may lead to additive effects in MDA accumulation, mitochondria lesions, whereas synergistic effects on cellular synthesis of proteins and DNA are observed (Traoré et al, 1999(Traoré et al, , 2000.…”

Section: Introductionmentioning

confidence: 99%

“…This toxin is also a tumor promoter (Fujiki and Suganuma, 1999), and an inducer of lipid peroxidation and apoptosis in cultured mammalian cells (Guzman and Castro, 1991;Boe et al, 1991;Matias et al, 1999a;Traoré et al, 1999Traoré et al, , 2000Lerga et al, 1999;Berven et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Traoré et al (1999) showed that among these metals cadmium increased the cytotoxicity of low concentrations of OA in a synergistic way. These metals can also cause lipid peroxidation, DNA damage, cell cycle arrest, and apoptosis (Boe et al, 1991;Traoré et al, 1999Traoré et al, , 2000Leonard et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Combined cytotoxicity and genotoxicity of a marine toxin and seafood contaminant metal ions (chromium and cadmium)

Souid-Mensi

Moukha

Maaroufi

et al. 2008

Environmental Toxicology

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Algal bloom with consequent production of marine toxins contaminating bivalves is increasing in costal regions worldwide because of sea water quality worsening. Contamination of seafood by diarrheic shellfish poisoning toxins (DSP) together with metals is frequently reported, a phenomenon not fully explained yet. In this context, metal ions were assayed in clams collected from the banned area of Boughrara, Tunisia, contaminated by Gymnodinium and other algae such as Dinophysis sp, accumulated by these bivalves. The presence of toxic metals ions such as Chromium (Cr) and Cadmium (Cd) in meat, shells, and water released by the clams prompted us to experiment in Caco-2 intestinal cell line toxic effects of these heavy metals ions in combination with okadaic acid, one DSP present in clams to assess the potential global toxicity. Cr and Cd produce additive effects in (i) reactive oxygen species production, (ii) cytotoxicity as assessed by the mitochondrial activity testing method (MTT test), and (iii) DNA lesions evaluated by agarose gel electrophoresis and acridine orange staining. Exaggerated DNA fragmentation is observed, suggesting an overloading of repair capacity of Caco-2 cells. The apoptosis suggested by a DNA fragment sizing (180-200 bp) in agarose gel and mechanisms underlying these additive effects in Caco-2 cells still need to be more comprehensively explained.

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Section: Discussionmentioning

confidence: 46%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Combined cytotoxicity and genotoxicity of a marine toxin and seafood contaminant metal ions (chromium and cadmium)

Souid-Mensi

Moukha

Maaroufi

et al. 2008

Environmental Toxicology

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Food Web and Ecosystem Impacts of Harmful Algae

Burkholder

Shumway

Glibert

2018

Harmful Algal Blooms

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Evaluation of the cytotoxicity and genotoxicity of extracts of mussels originating from Moroccan Atlantic coast, in human colonic epithelial cells Caco‐2

Nasser

Moustaid²,

Moukha

et al. 2008

Environmental Toxicology

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Industrial processing of phosphates generates chemical wastes which are, without any treatment, discharged directly into the Atlantic Ocean at Jorf Lasfar (JL), located 120 km south of Casablanca (Morocco) were shellfish are also collected by people without any control. Marine bivalves concentrate these pollutants by filtration and serve as vectors in human's exposure. The objective of this study was to test and compare in vitro on human intestinal cells (Caco-2) the cytotoxicity and genotoxicity of mussels (Mytilus galloprovincialis) extracts (either hydrophilic or lipophilic) collected at two coastal sites; JL (neighboring a phosphate processing plat-form) and Oualidia (OL) (a vegetable growing area) located 160 km south of Casablanca (i.e. 40 km south of JL). Using Caco-2 cells, the following end-points have been evaluated, cytotoxicity as measured by MTS test, inhibition of cellular macromolecules syntheses (DNA and protein) and genotoxicity evaluated by DNA fragmentation in agarose gel electrophoresis. The results indicated, that hydrophilic and lipophilic OL mussels extracts are cytotoxic and inhibit cellular macromolecules syntheses. Moreover these extracts damage the DNA in Caco-2 cells. The lipophilic JL mussels extract is cytotoxic, inhibits cellular macromolecules syntheses, and damages the DNA in Caco-2 cells whereas the hydrophilic extract of JL mussels fails to inhibit protein synthesis and does not damage the DNA. This extract rather enhances protein synthesis, suggesting possible metallothioneins induction by metal ions. Altogether these in vitro data indicate that mussels collected from OL could be more harmful than those from JL even though the later is closer to the pollution site than OL. Nevertheless consumption of mussels from all these areas may present a risk for humans. Epidemiological studies will be needed for global risk assessment in humans living in these areas especially those consuming see food regularly.

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Synergistic effects of some metals contaminating mussels on the cytotoxicity of the marine toxin okadaic acid

Cited by 26 publications

References 0 publications

Combined cytotoxicity and genotoxicity of a marine toxin and seafood contaminant metal ions (chromium and cadmium)

Combined cytotoxicity and genotoxicity of a marine toxin and seafood contaminant metal ions (chromium and cadmium)

Food Web and Ecosystem Impacts of Harmful Algae

Evaluation of the cytotoxicity and genotoxicity of extracts of mussels originating from Moroccan Atlantic coast, in human colonic epithelial cells Caco‐2

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