Synergistic Pathogenicity of Porphyromonas gingivalis and Fusobacterium nucleatum in the Mouse Subcutaneous Chamber Model

Filifactor alocis Infection and Inflammatory Responses in the Mouse Subcutaneous Chamber Model

“…An inoculum of 10 9 bacteria was sufficient to reproducibly establish colonization of the chambers. This is consistent with the properties of other recognized periodontal pathogens, such as P. gingivalis and T. forsythia, which can also colonize subcutaneous chambers with inocula of 10 9 viable bacteria (25,26). The local infection with F. alocis was rapidly resolved, and bacterial levels in the chamber decreased after 2 h and were almost undetectable by 72 h. Over this time period, F. alocis spread systemically and colonized remote tissues, including the spleen, liver, lung, and kidney.…”

Distribution of Porphyromonas gingivalis fimA Genotypes in Chronic Apical Periodontitis Associated with Symptoms

“…Once this ''balance'' is disturbed, an acute inflammatory reaction will develop with severe symptoms, such as tenderness to percussion, swelling, and suppuration (22). Some studies have focused on the prevalence of P. gingivali in acute apical abscesses (15,23). Others have compared the frequency of P. gingivalis in chronic and acute infections (24).…”

“…Recent studies adopting both culture-dependent and culture-independent approaches have reported high prevalence (28%-44%) of P. gingivalis in primary endodontic infections (10-13) and a positive correlation between P. gingivalis and some clinical features, such as tenderness to percussion, purulent drainage, swelling, and abscess (10,14), suggesting an association of P. gingivalis with primary apical periodontitis (13,15). However, the relationship between P. gingivalis fimA genotypes and apical periodontitis remains unclear.…”

The Role of Aggregation in Fusobacterium nucleatum– induced Immune Cell Death

“…Indeed, F. nucleatum is one of the most commonly occurring bacterial species in the human gingival crevice (1). In root canals of untreated teeth as well as root-filled teeth with periapical lesions, F. nucleatum constitutes one of the most frequently isolated bacteria, and its pathogenicity has been shown to be synergistic with other oral anaerobes (2)(3)(4)(5)(6). By using immunohistologic techniques, Matsuo et al (7) demonstrated that F. nucleatum is the most abundant bacterium that invades dentinal tubules of the roots in untreated teeth with apical lesions.…”