Synergistic Pro-Apoptotic Effect of a Cyclic RGD Peptide-Conjugated Magnetic Mesoporous Therapeutic Nanosystem on Hepatocellular Carcinoma HepG2 Cells

Abstract

Numerous nanocarriers have been developed to deliver drugs for the treatment of hepatocellular carcinoma. However, the lack of specific targeting ability, the low administration efficiency, and insufficient absorption by hepatocellular carcinoma cells, severely limits the therapeutic effect of the current drugs. Therefore, it is still of great clinical significance to develop highly efficient therapies with few side effects for the treatment of hepatocellular carcinoma. Herein, we developed a highly effective nanocarrier, cyclic RGD peptide-conjugated magnetic mesoporous nanoparticles (RGDSPIO@MSN NPs), to deliver the chemotherapeutic drug doxorubicin (DOX) to human hepatocellular carcinoma HepG2 cells, and further explored their synergistic apoptosis-promoting effects. The results showed that the prepared RGDSPIO@MSN NPs had good stability, biosafety and drug-loading capacity, and significantly improved the absorption of DOX by HepG2 cells, and that the RGDSPIO@MSN@DOX NPs could synergistically promote the apoptosis of HepG2 cells. Thus, this cyclic RGD peptide-modified magnetic mesoporous silicon therapeutic nanosystem can be regarded as a potentially effective strategy for the targeted treatment of hepatocellular carcinoma.

Silica nanoparticles containing nano-silver and chlorhexidine to suppress Porphyromonas gingivalis biofilm and modulate multispecies biofilms toward healthy tendency

“…Modified nMS could deliver drugs into microorganisms and target specific tissue for the responsive release [ 25–29 ]. This study loaded Chx and nAg on the nMS surface or inside the pores (nMS-nAg-Chx), which received synergistic antibacterial effects, providing a potential strategy for drug resistance of Chx long-term use.…”

“…Besides, biofilm microenvironments prevent antimicrobial diffusion through the deeper layers of the matrix, which reduces the activity of potent agents [ 24 ]. nMS have been applied as the carrier to deliver drugs into microorganisms and improve antimicrobial performance, which encapsulate agents and target specific tissue to keep a sustained drug concentration in lesion [ 25–29 ].…”

Magnetic nanosystem a tool for targeted delivery and diagnostic application: Current challenges and recent advancement

“…The outcomes demonstrated that the RGDSPIO@MSN@DOX NPs could synergistically increase the death of HepG2 cells and had good stability, biosafety, and drug-loading capability. They also considerably boosted the absorption of DOX by HepG2 cells ( Zhao et al, 2023 ).…”