Synergistic Regulation of Competence Development in<i>Bacillus subtilis</i>by Two Rap-Phr Systems

Bongiorni, Cristina; Ishikawa, Shu; Stephenson, Sophie; Ogasawara, Nagahisa; Perego, Marta

doi:10.1128/jb.187.13.4353-4361.2005

Cited by 91 publications

(112 citation statements)

References 49 publications

Supporting

Mentioning

104

Contrasting

Unclassified

Order By: Relevance

“…ComA, a response regulator of the ComA/ComP two-component system, activates the development of competence in B. subtilis. Similar to the role of Salmonella EIIA Ntr for SsrB, the Rap protein family members RapC, RapF, and RapH bind to the ComA protein and inhibit its binding to target promoter DNA (27,29,30). The kdpFABC operon encodes the high-affinity K + transporter KdpFABC, and its expression is regulated by the KdpD/ KdpE two-component system.…”

Section: Discussionmentioning

confidence: 99%

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Choi

Shin

Yoon

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

SsrA/SsrB is a primary two-component system that mediates the survival and replication of Salmonella within host cells. When activated, the SsrB response regulator directly promotes the transcription of multiple genes within Salmonella pathogenicity island 2 (SPI-2). As expression of the SsrB protein is promoted by several transcription factors, including SsrB itself, the expression of SPI-2 genes can increase to undesirable levels under activating conditions. Here, we report that Salmonella can avoid the hyperactivation of SPI-2 genes by using ptsN-encoded EIIA Ntr , a component of the nitrogen-metabolic phosphotransferase system. Under SPI-2-inducing conditions, the levels of SsrB-regulated gene transcription increased abnormally in a ptsN deletion mutant, whereas they decreased in a strain overexpressing EIIA Ntr . We found that EIIA Ntr controls SPI-2 genes by acting on the SsrB protein at the posttranscriptional level. EIIA Ntr interacted directly with SsrB, which prevented the SsrB protein from binding to its target promoter. Finally, the Salmonella strain, either lacking the ptsN gene or overexpressing EIIA Ntr , was unable to replicate within macrophages, and the ptsN deletion mutant was attenuated for virulence in mice. These results indicated that normal SPI-2 gene expression maintained by an EIIA Ntr -SsrB interaction is another determinant of Salmonella virulence.virulence gene regulation | nitrogen-metabolic phosphotransferase system (PTS) | protein-protein interaction

show abstract

Section: Discussionmentioning

confidence: 99%

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Choi

Shin

Yoon

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Despite their sequence and structural similarity to the Rap proteins that dephosphorylate Spo0F, some B. subtilis Rap proteins, such as RapC, RapF, and RapG, are transcriptional antiactivator proteins that inhibit the function of their response regulator tar-gets without dephosphorylating their receiver domains (20)(21)(22)(23). For example, RapC and RapF bind to the DNA binding domain of the response regulator ComA (20,23) and RapG binds to DegU (22).…”

mentioning

confidence: 99%

“…For example, RapC and RapF bind to the DNA binding domain of the response regulator ComA (20,23) and RapG binds to DegU (22). We previously showed that RapF functions mechanistically to sterically block the interaction of ComA with DNA promoters and disrupt the formation of active ComA dimers (21).…”

mentioning

confidence: 99%

A Plasmid-Encoded Phosphatase Regulates Bacillus subtilis Biofilm Architecture, Sporulation, and Genetic Competence

et al. 2013

View full text Add to dashboard Cite

bBacillus subtilis biofilm formation is tightly regulated by elaborate signaling pathways. In contrast to domesticated lab strains of B. subtilis which form smooth, essentially featureless colonies, undomesticated strains such as NCIB 3610 form architecturally complex biofilms. NCIB 3610 also contains an 80-kb plasmid absent from laboratory strains, and mutations in a plasmid-encoded homolog of a Rap protein, RapP, caused a hyperrugose biofilm phenotype. Here we explored the role of rapP phrP in biofilm formation. We found that RapP is a phosphatase that dephosphorylates the intermediate response regulator Spo0F. RapP appears to employ a catalytic glutamate to dephosphorylate the Spo0F aspartyl phosphate, and the implications of the RapP catalytic glutamate are discussed. In addition to regulating B. subtilis biofilm formation, we found that RapP regulates sporulation and genetic competence as a result of its ability to dephosphorylate Spo0F. Interestingly, while rap phr gene cassettes routinely form regulatory pairs; i.e., the mature phr gene product inhibits the activity of the rap gene product, the phrP gene product did not inhibit RapP activity in our assays. RapP activity was, however, inhibited by PhrH in vivo but not in vitro. Additional genetic analysis suggests that RapP is directly inhibited by peptide binding. We speculate that PhrH could be subject to posttranslational modification in vivo and directly inhibit RapP activity or, more likely, PhrH upregulates the expression of a peptide that, in turn, directly binds to RapP and inhibits its Spo0F phosphatase activity.

show abstract

“…Very recently, it was shown that also PhrF and PhrK stimulate ComA"s activity by directly inhibiting the activity of their cognate proteins RapF and RapK Bongiorni et al, 2005;Auchtung et al, 2006). Auchtung and his co-workers also shown that PhrC, PhrF, and PhrK stimulate ComA-dependent gene expression to different magnitudes and that all three peptides are reguired for full expression of ComA-dependent genes.…”

Section: Microbially Synthesized Bioactive Peptides By the Genus Bacimentioning

confidence: 99%

Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis

Özcengiz¹,

Taşkin

Demir³

et al. 2014

New Biotechnology

View full text Add to dashboard Cite

show abstract

Synergistic Regulation of Competence Development inBacillus subtilisby Two Rap-Phr Systems

Cited by 91 publications

References 49 publications

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

A Plasmid-Encoded Phosphatase Regulates Bacillus subtilis Biofilm Architecture, Sporulation, and Genetic Competence

Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis

Contact Info

Product

Resources

About

Synergistic Regulation of Competence Development inBacillus subtilisby Two Rap-Phr Systems

Cited by 91 publications

References 49 publications

Salmonella pathogenicity island 2 expression negatively controlled by EIIA Ntr –SsrB interaction is required for Salmonella virulence

Salmonella pathogenicity island 2 expression negatively controlled by EIIA Ntr –SsrB interaction is required for Salmonella virulence

A Plasmid-Encoded Phosphatase Regulates Bacillus subtilis Biofilm Architecture, Sporulation, and Genetic Competence

Proteome-wide analysis of the functional roles of bacilysin biosynthesis in Bacillus subtilis

Contact Info

Product

Resources

About

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence

Salmonella pathogenicity island 2 expression negatively controlled by EIIA ^Ntr –SsrB interaction is required for Salmonella virulence