Synergistically catalytic nanozymes based on heme-protein active site model for dual-signal and ultrasensitive detection of H2O2 in living cells

Hu, Xiaojun; Chen, Jie; Hu, Ruhui; Zhu, Zhikang; Lai, Zhaojia; Zhu, Xiaoyu; Zhu, Han; Koh, Kwangnak; Chen, Hongxia

doi:10.1016/j.snb.2021.129564

Cited by 21 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effectiveness of the proposed biosensors, as evidenced by the calculated K M app values (summarized in Table 2 ), are comparable to or lower than those previously reported in the literature for similar systems that present good response for the electrocatalysis of H 2 O 2 and that involve the use of hemeproteins: Hb-cAuNP/GCE (1.2 mM) [ 21 ], Mb-AuNPs-CNs/GCE (0.3 mM) [ 60 ], and Cyt c/AuNP/carbon paste (2.3 mM) [ 61 ] within others [ 24 , 62 ]. In addition, it is noteworthy that the values presented in this work were better than those reported for nanozymes based on hemeprotein active sites for the detection of H 2 O 2 in living cells [ 63 ]. Thus, the obtained results indicated that the biosensors maintain a higher biological affinity to H 2 O 2 because the hemeproteins retain their catalytic activity.…”

Section: Resultsmentioning

confidence: 66%

Behind the Optimization of the Sensor Film: Bioconjugation of Triangular Gold Nanoparticles with Hemoproteins for Sensitivity Enhancement of Enzymatic Biosensors

et al. 2023

View full text Add to dashboard Cite

Electrochemical biosensors are widely used in a multitude of applications, such as medical, nutrition, research, among other fields. These sensors have been historically used and have not undergone many changes in terms of the involved electrochemical processes. In this work, we propose a new approach on the immobilization and enhancement of the electrochemical properties of the sensing layers through the control and bioconjugation of hemoproteins (hemoglobin, myoglobin, and cytochrome C) on anisotropic gold nanoparticles (gold nanotriangles (AuNTs)). The hemeproteins and the AuNTs are mixed in a solution, resulting in stable bioconjugates that are deposited onto the electrode surface to obtain the biosensors. All the systems proposed herein exhibited direct well-defined redox responses, highlighting the key role of the AuNTs acting as mediators of such electron transfers. Several protein layers surrounding the AuNTs are electroactive, as demonstrated from the charge measured by cyclic voltammetry. The retention of the stability of the hemeproteins once they are part of the bioconjugates is evidenced towards the electrocatalytic reduction of hydrogen peroxide, oxygen, and nitrite. The parameters obtained for the proposed biosensors are similar or even lower than those previously reported for similar systems based on nanomaterials, and they exhibit attractive properties that make them potential candidates for the latest developments in the field of sensing devices.

show abstract

Section: Resultsmentioning

confidence: 66%

Behind the Optimization of the Sensor Film: Bioconjugation of Triangular Gold Nanoparticles with Hemoproteins for Sensitivity Enhancement of Enzymatic Biosensors

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The hemeprotein mimics can catalyze the oxidation of a variety of the chromogenic substrates (e.g., TMB, ABTS, OPD, amplex red) in the presence of H 2 O 2 , which can be monitored by the spectrophotometric or spectrofluorometric methods, and applied for the detection of the biologically significant signaling agents. Chen and co-workers reported a peroxidase-like nanozyme (FeP-pSC4-AuNPs) with a 2D nanonetwork structure by assembling calixarene (pSC4) modified gold nanoparticles with iron porphyrins, which showed the application of in situ colorimetric or electrochemical detection of H 2 O 2 in living cells. Shi and co-workers used block copolymer poly(ethylene glycol)- block -poly(4-vinylpyridine) (PEG- b -P4VP) to coordinate with hemin and obtained peroxidase mimics with a micellar structure, which was employed for detection of H 2 O 2 and degradation of azo dyes and phenols.…”

Section: Applications Of the Hemeprotein Mimicsmentioning

confidence: 99%

Heme-Dependent Supramolecular Nanocatalysts: A Review

Liu

Wang

2023

ACS Nano

View full text Add to dashboard Cite

Enzymes fold into three-dimensional structures to distribute amino acid residues for catalysis, which inspired the supramolecular approach to construct enzyme-mimicking catalysts. A key concern in the development of supramolecular strategies is the ability to confine and orient functional groups to form enzyme-like active sites in artificial materials. This review introduces the design principles and construction of supramolecular nanomaterials exhibiting catalytic functions of heme-dependent enzymes, a large class of metalloproteins, which rely on a heme cofactor and spatially configured residues to catalyze diverse reactions via a complex multistep mechanism. We focus on the structure–activity relationship of the supramolecular catalysts and their applications in materials synthesis/degradation, biosensing, and therapeutics. The heme-free catalysts that catalyze reactions achieved by hemeproteins are also briefly discussed. Towards the end of the review, we discuss the outlook on the challenges related to catalyst design and future prospective, including the development of structure-resolving techniques and design concepts, with the aim of creating enzyme-mimicking materials that possess catalytic power rivaling that of natural enzymes..

show abstract

“…Compared with enzymatic biosensors, the Fe 3 O 4 -based sensor exhibited outstanding stability [58]. Moreover, because of the high sensitivity of electrochemical biosensors, extracellular H 2 O 2 with a low concentration released from cells under stimulation was successfully monitored [59][60][61][62]. For example, electrochemical sensing of H 2 O 2 was performed by using Au nanoflowers/Fe 3 O 4 @ZIF-8-MoS 2 nanocomposites as peroxidase mimics and H 2 O 2 concentration released from H9C2 cardiac cells under ascorbic acid (AA) stimulation was successfully determined (Figure 4A and 4B) [59].…”

Section: Small Moleculesmentioning

confidence: 99%

Recent Advances on Nanozyme‐based Electrochemical Biosensors

2022

View full text Add to dashboard Cite

Nanozymes are nanomaterials with enzyme-like catalytic activities. The unique features of nanozymes (such as high stability, low cost, large surface area for bioconjugation, ease of storage, and multi-functionalities) offer unprecedented opportunities for designing electrochemical biosensors. Recent years have witnessed the rapid development of nanozyme-based electrochemical biosensors. To highlight these achievements, this review first discusses the representative nanozymes including peroxidase mimics, oxidase mimics, hydrolase mimics, and superoxide dismutase mimics used in electrochemical biosensors. Then, it summarizes the bioanalytical applications for the detection of various analytes. Finally, current challenges and future research directions are summarized.

show abstract

Synergistically catalytic nanozymes based on heme-protein active site model for dual-signal and ultrasensitive detection of H2O2 in living cells

Cited by 21 publications

References 36 publications

Behind the Optimization of the Sensor Film: Bioconjugation of Triangular Gold Nanoparticles with Hemoproteins for Sensitivity Enhancement of Enzymatic Biosensors

Behind the Optimization of the Sensor Film: Bioconjugation of Triangular Gold Nanoparticles with Hemoproteins for Sensitivity Enhancement of Enzymatic Biosensors

Heme-Dependent Supramolecular Nanocatalysts: A Review

Recent Advances on Nanozyme‐based Electrochemical Biosensors

Contact Info

Product

Resources

About