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Herein, we report a self-assembled fabrication of a network nanozyme (TMPyP-pSC4@GNPs) and a glucose-recognition molecule (APBA) for highly stable and selective glucose sensing. By involving the derivative of water-soluble porphyrin in TMPyP-pSC4@GNPs, the peroxidase-like catalytic performance of the nanozyme was improved benefiting from the topological structure via high electronic transfer and large surface area. Besides, the hybrid of APBA in TMPyP-pSC4@GNPs can increase the selectivity due to its specific binding affinity with glucose, and the detection signal was enhanced by 73.2%. The excellent stability and selectivity meet the need of clinically detecting glucose in urine and blood. Therefore, this mechanistically novel strategy expands the scope of the traditional GNPs-based catalytic strategy of glucose and serves as an alternative and potential glucose detection platform.

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