Synergy among thymol, eugenol, berberine, cinnamaldehyde and streptomycin against planktonic and biofilm-associated food-borne pathogens

Liu, Q.; Niu, Hong; Zhang, W.; Mu, Haibo; Sun, Chunli; Duan, Jinyou

doi:10.1111/lam.12401

Cited by 99 publications

(61 citation statements)

References 53 publications

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“…The combinations of TC and antibiotics can be used as an alternative therapeutic application, which can decrease the minimum effective dose of the drugs, thus reducing their possible adverse effects and the costs of treatment. Reduction of antibiotic concentration may help to prevent the spread of antibiotic resistance [26,29,35,36]. In the case of the weakly adhering strain, reduction of biofilm metabolic activity at the presence of 1/2 TC MBIC after 3, 6 and 8 h did not significantly differ from the strongly adhering strain.…”

Section: Discussionmentioning

confidence: 93%

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

Kot

Sytykiewicz

Sprawka

et al. 2019

IJMS

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The effects of trans-cinnamaldehyde (TC) on transcriptional profiles of biofilm-associated genes and the metabolic activity of two methicillin-resistant Staphylococcus aureus (MRSA) strains showing a different degree of adherence to polystyrene, were evaluated. Metabolic activity of S. aureus in biofilm was significantly decreased in the presence of TC at 1/2 minimum biofilm inhibition concentration (MBIC). Expression levels of the genes encoding laminin binding protein (eno), elastin binding protein (ebps) and fibrinogen binding protein (fib) in the presence of TC at 1/2 MBIC were lower than in untreated biofilm in both the weakly and strongly adhering strain. The highest decrease of expression level was observed in case of fib in the strongly adhering strain, in which the amount of fib transcript was 10-fold lower compared to biofilm without TC. In the presence of TC at 1/2 MBIC after 3, 6, 8 and 12 h, the expression level of icaA and icaD, that are involved in the biosynthesis of polysaccharide intercellular adhesin, was above half lower in the weakly adhering strain compared to biofilm without TC. In the strongly adhering strain the highest decrease in expression of these genes was observed after 3 and 6 h. This study showed that TC is a promising anti-biofilm agent for use in MRSA biofilm-related infections.

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Section: Discussionmentioning

confidence: 93%

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

Kot

Sytykiewicz

Sprawka

et al. 2019

IJMS

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“…MTT assay showed that carvacrol at MIC, 2 x MIC and 4 x MIC decreased S. Typhimurium biofilm activity to 0.089 OD 550, 0.076 OD 550 and 0.053 OD 550 respectively, as compared with 0.142 OD 550 for the control (p < 0,05) (Figure 4). Previous studies have demonstrated the effect of different essential oils and their components on the reduction metabolic activity of Gram-positive and Gramnegative bacterial biofilms (Bai, Vittal, 2014;Piovezan et al, 2014;Liu et al, 2015b) but to our knowledge, no studies have evaluated the effect of carvacrol on the metabolic activity on Gram-negative bacteria.…”

Section: Effect Of Carvacrol In Biofilmmentioning

confidence: 99%

Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium

Trevisan

Silva

Negri

et al. 2018

Braz. J. Pharm. Sci.

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The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and the time-kill curve and scanning electron microscopy (SEM) were performed to evaluate antibacterial activity. Antibiofilm activity was evaluated by quantifying total biomass using crystal violet assay, and metabolic activity was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide] assay. The action of carvacrol against preformed biofilm on polypropylene and stainless steel was also evaluated by colony counting and SEM. The MIC and MBC was 312 µg mL-1. Carvacrol at MIC and 2 x MIC eliminated cells after 6 and 1 h of treatment, respectively, as exhibited in the time-kill curve. The greatest reduction in biofilm biomass and metabolic activity was 1,719 OD 550 and 0,089 OD 550 respectively, both at 4 x MIC of carvacrol. In carvacrol treated biofilms of S. Typhimurium on polypropylene, a reduction of 5.12 log was observed with 4 x MIC, while on stainless steel, carvacrol at 4 x MIC reduced bacterial counts by 5 log. The results showed that carvacrol exhibits antibacterial activity and can be used as an alternative for the control of S. Typhimurium biofilms.

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“…In particular, twenty-five L. monocytogenes isolates from a variety of food samples and five reference strains were analyzed. The interference of thymol with the growth of L. monocytogenes has been recognized for a long time [18][19][20], but as far as we know, it is the first time that a good number of food isolates were tested for susceptibility to thymol. The aim of the study was to suggest and support the replacement or reduction of the use of antibiotics in food processing settings and in farms.…”

Section: Introductionmentioning

confidence: 99%

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

Cusimano¹,

Stefano²,

Giglia

et al. 2020

Molecules

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The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum β-lactamase (ESBL) genes. All isolates and reference strains were susceptible to thymol at Minimum inhibitory concentration (MIC) values ranging from 250 to 800 μg/mL. An interesting activity of interference with biofilm formation of L. monocytogenes and E. coli was found for thymol at sub-MIC concentrations of 200, 100, 75, and 50 μg/mL. Anti-biofilm activity ranging from 59.71% to 66.90% against pre-formed 24-h-old L. monocytogenes biofilms at concentrations of 500 or 800 µg/mL, corresponding to 2× MIC, was determined against free-living forms of six isolates chosen as the best or moderate biofilm producers among the tested strains. The property of thymol to attack L. monocytogenes biofilm formation was also observed at a concentration of 100 µg/mL, corresponding to 1/4 MIC, by using a stainless-steel model to simulate the surfaces in food industries. This study gives information on the use of thymol in food processing setting.

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Synergy among thymol, eugenol, berberine, cinnamaldehyde and streptomycin against planktonic and biofilm-associated food-borne pathogens

Cited by 99 publications

References 53 publications

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

Effect of trans-Cinnamaldehyde on Methicillin-Resistant Staphylococcus aureus Biofilm Formation: Metabolic Activity Assessment and Analysis of the Biofilm-Associated Genes Expression

Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium

Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings

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