Synnemata Production Using Silkworm Variety, Female Yangwonjam by Isaria tenuipes

Abstract

This study was conducted to confirm the possible use of female Yangwonjam as a host for synnemata production of Isaria tenuipes in eight local areas in Korea. Silkworm pupation rate, infection rate and synnemata characteristics of I. tenuipes were examined. Normal silkworms had a higher pupation rate than silkworms inoculated with I. tenuipes. The pupae survival percentage of normal silkworm in cocoons was 92.5~97.6%, whereas it ranged from 91.1~95.6% in silkworms sprayed with I. tenuipes. Female Yangwonjam showed the highest survival percentage at 97.6% among the silkworm varieties tested. I. tenuipes infection rate of larvae of 5th instar newly-exuviated silkworms was 89.2~90.7% in the spring rearing season and 98.2~99.3% in the autumn rearing season. Synnemata production of I. tenuipes was excellent in female Yangwonjam with an incidence rate of 98.0% followed by male Yangwonjam (94.1%) and Baegokjam (93.3%) in the spring rearing season. Synnemata living weight ranged from 1.44~0.94 g in the spring rearing season. The female Yangwonjam had the heaviest synnemata weight (1.44 g) in the spring rearing season. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and were similar in shape and color to wild synnemata collected in Korea.

First record of epizootics in the ocola skipper,Panoquina ocola(Lepidopera: Hesperiidae), caused byIsaria tenuipesin flooded rice fields of Central Brazil

“…Other studies support this claim, as seen by Ji et al . () with silkworm larvae inoculated with I. tenuipes inducing high mortality followed by production of synnemata in pupae (>90% mycosis occurred during pupal stage). These same authors devised an in vivo method to produce synnemata of I. tenuipes using silkworm pupae as the preferred form of the fungus for the extraction of adenosine and N6‐(2‐hydroxyethyl) adenosine, which are greatly valued for their biological and pharmaceutical properties in the folk medicinal traditions in East Asian countries.…”

“…Upon host death, the fungus emerges from the cadaver to produce very conspicuous fruiting bodies (fascicles of hyphae referred to as synnemata) that bear powdery masses of asexual spores (conidia) (Fukatsu et al 1997). In Korea, for example, the fruiting bodies of this fungus are also known as snowflakes 'Dongchunghacho' due to their milky-white appearance (Ji et al 2011). We report a serendipitous discovery and isolation of a previously unrecorded insect pathogen, and the morphological and genomic characterizations of I. tenuipes from populations of Panoquina ocola infesting flooded rice fields in the Central Brazilian State of Goi as.…”

“…In Korea, for example, the fruiting bodies of this fungus are also known as snowflakes ‘Dongchunghacho’ due to their milky‐white appearance (Ji et al . ). We report a serendipitous discovery and isolation of a previously unrecorded insect pathogen, and the morphological and genomic characterizations of I. tenuipes from populations of Panoquina ocola infesting flooded rice fields in the Central Brazilian State of Goiás.…”

Isaria tenuipes Peck, an entomopathogenic fungus from Darjeeling Himalaya: Evaluation of in-vitro antiproliferative and antioxidant potential of its mycelium extract

“…Isaria tenuipes has been used as folk medicine or health food in Japan, China, and South Korea [12]. It is being used for hundreds of years as a food ingredient to strengthen the immune system and regain energy [13]. In the Eastern Himalayan region, Isaria tenuipes is traditionally used as a tonic and food supplement for recovery from tuberculosis and for speedy recuperation after childbirth.…”

Kidney Toxicity Induced by 13 Weeks Exposure to the Fruiting Body of Paecilomyces sinclairii in Rats

“…Especially, cordyceps formed from silk worms larvae have been used as a traditional folk remedy or food ingredient to strengthen the immune function and regain vitality in Asian countries including China, Japan and Korea (Ji et al ., 2011) .…”