The deep structure specification consists of a control verb and a series of case roles which are marked in the Noun Phrase (NP). Study about the deep structure specification for the semantic prime DO is related to the case grammar of which model is determined by the syntactic frames of the semantic prime DO. Therefore, this article tries to describe the syntactic frames and the case roles of the semantic prime DO. Data were collected from four Balinese folklores with observation method and note taking technique. The Descriptive qualitative method was used to analyze the data collected. The results of the analysis showed that the syntactic frames of the semantic prime DO can be described as (1) X does (something) in which the deep structure can be