Synteny between Zoysia japonica Steud. and Oryza sativa L. based on RFLP, CISP and PLUG markers

Abstract

In order to study the synteny between Zoysia japonica and Oryza sativa, we added three types of markers including Restriction fragment length polymorphism, Conserved intron scanning primers and PCR-based landmark unique gene in our previous high-density SSR-based linkage map. A total of 100 loci showed homology to the rice genome were merged into our previously constructed SSR-based linkage map of zoysiagrass. The new map was longer than all zoysiagrass genetic maps reported previously, and contains the number of linkage groups 20, which is equal to the haploid number of chromosomes of zoysiagrass. The new mapped 100 homologous loci were mapped to 19 linkage groups (LGs) except of LG 20, of them, the LGs 2, 8 and 13 of zoysiagrass showed colinearity with chromosomes 3, 6 and 1 of rice respectively, and otherLGs of zoysiagrass contained variable numbers of markers that showed homology with the rice genome. These results suggested a syntenic relationship between zoysiagrass and rice. The improved genetic linkage map and the new markers developed in this study will serve as reference genetic linkage maps for zoysiagrass species and will be useful for mapping QTLs associated with various traits, and for improving zoysiagrass breeding programs.

Population genetic structure and evolutionary history of Psammochloa villosa (Trin.) Bor (Poaceae) revealed by AFLP marker

“…We used AFLPs because they remain extremely efficient for investigating genetic diversity, genetic structure, and population demography due to their high levels of polymorphism (Wang, Wang, Liu, Yang, & Chen, 2008), their reproducible, reliable re-sults that are unaffected by the developmental stage of plant materials, and their universality among plant species. In addition, they have been used to resolve genetic structures and population demography in many diverse grass species such as Oryza sativa , Leymus racemosus , Orinus thoroldii and O. kokonoricus (Zhang & Jia, 2002;Sim, 2005;Li, 2015;Cai, 2017;Liu, Harris, Gao, Su, & Ren, 2019). Our main objectives were to (1) analyze the genetic structure from 43 populations of P. villosa from Inner Mongolian Plateau using an AFLP dataset, (2) test whether historical genetic divergence occurred among populations in response to Quaternary climate oscillations, and (3) evaluate the abiotic factors that are most influential in driving the distributions of P. villosa .…”