Synthesis and Antimicrobial Activity of a Silver‐Hydroxyapatite Nanocomposite

Abstract

A silver-hydroxyapatite nanocomposite has been obtained by a colloidal chemical route and subsequent reduction process in H2/Ar atmosphere at350∘C. This material has been characterized by TEM, XRD, and UV-Visible spectroscopy, showing the silver nanoparticles (∼65 nm) supported onto the HA particles (∼130 nm) surface without a high degree of agglomeration. The bactericidal effect against common Gram-positive and Gram-negative bacteria has been also investigated. The results indicated a high antimicrobial activity forStaphylococcus aureus, PneumococcusandEscherichia coli,so this material can be a promising antimicrobial biomaterial for implant and reconstructive surgery applications.

Antimicrobial activity of hemocompatible silver doped hydroxyapatite nanoparticles synthesized by modified sol–gel technique

“…Silver aggregates on HAp particles have been reported during synthesis of Ag-HAp nanocomposites, using a colloidal chemical route and subsequent reduction in H 2 /Ar atmosphere at 350°C. This has been attributed to partial destabilization of HAp in aqueous suspension favoring the growth of Ag 2 O nuclei (Diaz et al 2009). Chung et al (2006) also reported the presence of Ag 2 O crystallite phase in addition to the HAp and the nitrate phases, depending upon the degree of substitution by silver.…”

“…Antimicrobial activity for Ag nanoparticles is reported in the range 1.69-100 lg/ml for various bacterial cultures (MarambioJones and Hoek 2010; Kvitek et al 2008;Kim et al 2011;Sondi and Sondi 2004). Silver based HAp nanocomposites have been shown to exhibit antimicrobial activity in growth medium where the Ag concentration ranges from 10 to 70 lg/ml for Gram-negative and Gram-positive cultures (Diaz et al 2009;Rameshbabu and Prasad Rao 2007). In this study, Ag-HAp exhibited antimicrobial activity at a much lower silver concentration of 7 lg/ml against E. coli and at 15 lg/ml for S. aureus.…”

“…Due to its high osteoconductive properties, porosity, and longer degradation times, it finds application as an attractive implant material; as filler in dental prosthesis, for reconstructing bone defects, as thin coatings on metals like titanium or CoCrMo alloys for hip and knee prosthesis (Diaz et al 2009). Its high biocompatibility, allows tissue to infiltrate and chemically bond with it.…”

“…Silver ions in particular exhibit oligodynamic activity with a broad spectrum of antibacterial susceptibility which is particularly effective against the polymicrobial colonization associated with biomaterial infection (Gristina and Costerton 1985;Kramer et al 1981) Silver containing antimicrobial biomaterials have silver in the form of elemental silver or Ag ions incorporated into organic (polymers) or inorganic (bioglasses and HAp) matrices (Diaz et al 2009). Ag ions released from matrices are absorbed into the surface of negatively charged bacterial cell walls due to electrostatic attraction leading to the disruptions of the cell wall and cell membrane (Woo et al 2008).…”

“…(Diaz et al 2009;Rameshbabu and Prasad Rao 2007;Stanic et al 2011;Zheng et al 2009). In this study crystalline Ag-HAp nanorods have been synthesized at 100°C using a modified sol-gel method followed by dialysis (Jadalannagari et al 2011).…”