Synthesis and Antiplatelet Adhesion Behavior of a Poly(L-lactide-co-glycolide)–Poly(1,5-dioxepan-2-one) Multiblock Copolymer

Abstract

Platelet adhesion and denaturation on artificial medical implants induce thrombus formation. In this study, bioabsorbable copolymers composed of poly(L-lactide-co-glycolide) (PLGA) and poly(1,5-dioxepan-2-one) (PDXO) were synthesized and evaluated for their antiplatelet adhesive properties. The PLGA−PXO multiblock copolymer (PLGA−PDXO MBC) and its random copolymer (PLGA−PDXO RC) showed effective antiplatelet adhesive properties, and the number of adhered platelets was similar to those adhered on poly(2-methoxyethylacrylate), a known antiplatelet adhesive polymer, although a large number of denatured platelets were observed on a PLGA−poly(εcaprolactone) multiblock copolymer (PLGA−PCL MBC). Using monoclonal antifibrinogen IgG antibodies, we also found that both αC and γ-chains, the binding sites of fibrinogen for platelets, were less exposed on the PLGA−PDXO MBC surface compared to PLGA−PCL MBC. Furthermore, free-standing films of PLGA− PDXO MBC were prepared by casting the polymer solution on glass plates and showed good tensile properties and slow hydrolytic degradation in phosphate-buffered saline (pH = 7.4). We expect that the unique properties of PLGA−PDXO MBC, i.e., antiplatelet adhesive behavior, good tensile strength, and hydrolytic degradation, will pave the way for the development of new bioabsorbable implanting materials suitable for application at blood-contacting sites.

Cell adhesion behavior of poly(l‐lactide‐co‐glycolide)‐poly(1,5‐dioxepan‐2‐one) random multiblock copolymers

“…An alternative PLGAÀPDXO multiblock copolymer (PLGAÀPDXO MBC) was prepared by the reported procedure (PLGA: PDXO = 50:50, M w = 110,000, M w /M n = 1.57). 29 The PLGAÀPCL MBC was prepared by the procedure reported in our previous paper. 33 The degree of polymerization of PLGA (L-lactide:glycolide = 9: 1) and PCL was 25.1 and 28.1, respectively, as determined by 1 H NMR measurements.…”

“…We have also reported poly(L-lactide-co-glycolide)-poly(1,5-dioxepan-2-one) multiblock copolymer (PLGAÀPDXO MBC) as a bioabsorbable and antiplatelet adhesive polymer. 29 The poly(1,5-dioxepan-2-one) segment is a hydrophilic component of the multiblock copolymer. 30 Owing to the microphase-separated morphology caused by the multiblock structure, PLGAÀPDXO MBC shows good tensile properties.…”

Mechanisms Affecting Physical Aging and Swelling by Blending an Amphiphilic Component

“…For example, di-2-ethylhexyl phthalate (DEHP) was extensively added to soft polyvinyl chloride (PVC) medical products to reduce hardness, and its ability to migrate to fatty solutions (such as blood) was proved, which was harmful to the development and reproduction of mammals [11]. Therefore, new solutions are being developed worldwide, mainly including: (1) chemical modification such as polymer grafting [12] or copolymerization [13,14]; (2) physical modification such as polymer blending [15,16]. Compared with chemical modification, physical modification saves more time and money, has lower technical requirements, and is conducive to largescale production.…”

Polydioxanone Derivative Bearing Methoxy Groups toward Bio‐Functional Degradable Polymers Exhibiting Hydration‐Driven Biocompatibility

“…Multi‐block copolymers, including a PDX analogue, have recently been reported to exhibit antiplatelet adhesion properties. [ 31 ] In the present study, we designed and synthesized a PDX analogue with methoxy side chains ( 1 ) to enhance the hydration‐driven biocompatibility of PDX by the synergy of ether moieties in the main and side chains (Figure 1). As polymer 1 is derived from glycerol, an abundant biomass feedstock, as well as PMDO, the successful synthesis of 1 will extend to the further development of its analogues for applications in sustainable materials.…”