Synthesis and binding properties of new selective ligands for the nucleobase opposite the AP site

Deep-red fluorogenic cyanine dyes carrying an amino group-terminated side chain for improved RNA detection and nucleolar RNA imaging

“…Such a chemical modification was demonstrated to be effective for various nucleic acid-binding ligands because the amino groups show the favorable contribution into the binding with target nucleic acids through electrostatic interactions. 5 Quaternary (BIQ–N + Me 3 ) or primary amino group (BIQ–NH 2 ) was thus introduced into the quinoline ring of BIQ scaffold through a propyl spacer ( Fig. 1 ).…”

Antibiotic Drugs Aminoglycosides Cleave DNA at Abasic Sites: Shedding New Light on Their Toxicity?

“…Indeed, a number of anticancer drugs, mostly of the alkylating family, have been reported to generate abasic sites and are thus susceptible to potentiation by abasic site-specific molecules. 57,74,75 Sensitization of cancer cells to bis-chloroethylnitrosurea (BCNU), a clinically useful anticancer drug, by DTAC and ATAC has also been observed. 74 Therefore, compounds able to interfere with the abasic site repair are of great interest for diagnostic and therapeutic purposes in order to potentiate the cytotoxic effects of alkylating drugs or radiations.…”

“…74 Therefore, compounds able to interfere with the abasic site repair are of great interest for diagnostic and therapeutic purposes in order to potentiate the cytotoxic effects of alkylating drugs or radiations. 75 Here, we report on the study of the cleaving efficiency at abasic sites in DNA of 11 antibiotic AG drugs and of 4 synthesized derivatives. All compounds were tested on abasic lesions generated by controlled depurination of pBR322 plasmid DNA.…”

“…For instance, an inhibitory effect might be useful to sensitize tumor cells to alkylating drugs. Indeed, a number of anticancer drugs, mostly of the alkylating family, have been reported to generate abasic sites and are thus susceptible to potentiation by abasic site-specific molecules. ,, Sensitization of cancer cells to bis-chloroethylnitrosurea (BCNU), a clinically useful anticancer drug, by DTAC and ATAC has also been observed …”

A new strategy for site-specific alkylation of DNA using oligonucleotides containing an abasic site and alkylating probes

“…As a hydrophobic pocket, an AP site in duplex DNA has been configured in our study, because it has been reported that a variety of ligands can recognize the nucleobase opposite the AP site through hydrogen bonding even in the aqueous media. [17][18][19][20][21][22] In this study, we have designed the new probes for reactions involving an AP site in duplex DNA. These probes consist of 2-AVP as a reactive moiety and penta-arginine (2: (Arg) 5 ), acridine (3), or Hoechst (4) as a binding moiety with high affinity to duplex DNA (Fig.…”