Synthesis and characterisation of far-red fluorescent cyanine dye doped silica nanoparticles using a modified microemulsion method for application in bioassays

Nooney, Robert; O’Connell, Claire; Roy, S. C. Dutta; Boland, Karla; Keegan, Gemma; Kelleher, Susan M.; Daniels, Stephen; McDonagh, Colette

doi:10.1016/j.snb.2015.06.117

Cited by 27 publications

(17 citation statements)

References 43 publications

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“…In the Stöber method, ammonium hydroxide acts as a catalyst to bring about hydrolysis and condensation of a silica precursor in a water/ethanol mixture. Different molar ratios are used to control particle size during the Oswald ripening growth process [144]. For the microemulsion approach, hydrolysis and condensation occurs at the interface of a micelle-stabilised water droplet inside an oil phase.…”

Section: Discussionmentioning

confidence: 99%

Micro- and nanostructured sol-gel-based materials for optical chemical sensing (2005–2015)

2016

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This review (with 172 references) highlights the progress made in the past 10 years in silica sol-gel-based materials for use in optical chemical sensing. Following an introduction, the processes leading to the sol-gel-based and ormosil materials, their printability and methods for characterisation are discussed. Then various classes of optical sensors, with a focus on sensors for pH values, oxygen, carbon dioxide, ammonia (also in dissolved form), and heavy metal ions are described. A further section covers nanoparticle-based optical sensors mainly for use in intracellular sensing of the above species. Recent developments in this area are also emphasised and future trends discussed.

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Section: Discussionmentioning

confidence: 99%

Micro- and nanostructured sol-gel-based materials for optical chemical sensing (2005–2015)

2016

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“…However, this method is complicated and expensive because of the thiolated oligonucleotide. Other silica probes doped with far-red fluorescent cyanine dyes (Cy5 and FR670) were also reported [394]. At last, the MnO nanoparticles offer an excellent matrix to accommodate optical imaging modalities by coniugation NIR dyes [395].…”

Section: Silica Nanoparticles For Bioimaging In Therapymentioning

confidence: 98%

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Miksa¹

2016

Med chem (Los Angeles)

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This review highlights the role of fluorescent dyes as active "molecular photoswiches" focusing on the application in bioimaging and anticancer therapies in the field of therapeutic delivery. The author describes the development of prodrugs targeted to specific cell types and polymeric nanocarriers (capsules, micelles and silica nanoparticles) used as fluorescent probes which offer advantages in the integration of "smart" features of fluorescent dyes into synthetic materials. The incorporation of biologically responsive components that cleave upon changes in pH or light irradiation is fundamental to the successful design of such carriers with capabilities to load and release therapeutics specifically at a target site.

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“…Silica nanoparticles, which were doped with Cy5 dye, were synthesized using a microemulsion method that was developed by Nooney et al 18 . The surface of the nanoparticles was modified using a step by step approach 19 with a PAMAM dendrimer multivalent linker (PAMAM dendrimer, ethylenediamine core, generation 4.5 solution5 wt.…”

Section: Cy5 Doped Nanoparticle Synthesismentioning

confidence: 99%

Fluorescent Cy5 silica nanoparticles for cancer cell imaging

et al. 2015

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Cancer is a leading cause of death worldwide, with metastasis responsible for the majority of cancer-related deaths. Circulating tumour cells (CTCs) play a central role in metastasis. Fluorescent silica particles (NPs), of diameter ~50 nm which contain a large concentration of Cy5 dye molecules and are extremely bright, have been developed to detect these rare CTCs. Due to this brightness, the particles have superior performance compared to single Cy5 dye molecule labels, for detecting cancer cells. Fluorescence measurements show that the NPs are almost 100 times brighter than the free dye. They do not photobleach as readily and, due to the biocompatible silica surface, they can be chemically modified, layerby-layer, in order to bind to cells. The choice of these chemical layers, in particular the NP to antibody linker, along with the incubation period and type of media used in the incubation, has a strong influence on the specific binding abilities of the NPs. In this work, NPs have been shown to selectively bind to the MCF-7 cell line by targeting epithelial cellular adhesion molecule (EpCAM) present on the MCF-7 cell membrane by conjugating anti-EpCAM antibody to the NP surface. Results have shown a high signal to noise ratio for this cell line in comparison to a HeLa control line. NP attachment to cells was verified qualitatively with the use of fluorescence microscopy and quantitatively using image analysis methods. Once the system has been optimised, other dyes will be doped into the silica NPs and their use in multiplexing will be investigated.

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Synthesis and characterisation of far-red fluorescent cyanine dye doped silica nanoparticles using a modified microemulsion method for application in bioassays

Cited by 27 publications

References 43 publications

Micro- and nanostructured sol-gel-based materials for optical chemical sensing (2005–2015)

Micro- and nanostructured sol-gel-based materials for optical chemical sensing (2005–2015)

Fluorescent Dyes Used in Polymer Carriers as Imaging Agents in Anticancer Therapy

Fluorescent Cy5 silica nanoparticles for cancer cell imaging

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