Synthesis and characterisation of quantum dots coupled to mycolic acids as a water‐soluble fluorescent probe for potential lateral flow detection of antibodies and diagnosis of tuberculosis

Kabwe, Kapambwe P.; Nsibande, Sifiso A.; Lemmer, Yolandy; Pilcher, Lynne A.; Forbes, Patricia B.C.

doi:10.1002/bio.4170

Cited by 3 publications

(3 citation statements)

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“…It can be deduced that the MA‐GQDs were more dispersible in water as compared to the coupled material prepared with semiconductor quantum dots reported in our previous work. [ 22 ] As can be seen in the inset shown in Figure 10, all the sample at the spotting point eluted up the strip, while for the semiconductor based MA‐QDs, some spotted sample remained at the initial spotting point. Good solubility in water and low toxicity thus makes MA‐GQDs the preferred material to potentially detect anti‐MA antibodies and diagnosis of TB using the nitrocellulose membrane flow technique.…”

Section: Resultsmentioning

confidence: 99%

“…The fluorescence quantum yield of the GQDs and MA-GQDs is somewhat lower than the fluorescence quantum yield of semiconductor-based quantum dots and the corresponding MA-QDs reported in our previous work. [22] 3.4 | FTIR analysis of MA, GQDs and MA-GQDs…”

Section: Fluorescent Properties Of Gqds and Ma-gqdsmentioning

confidence: 99%

“…In this study, we present for the first time the linking of chloroform-soluble MAs to water-soluble GQDs to form MA-GQDs and show that this enhances the solubility of MAs, and enables their lateral flow, whilst also reducing the toxicity of the sensor material as compared to semiconductor-based quantum dots, which we have reported on previously. [22] F I G U R E 1 Schematic of the hydrothermal synthesis of GQDs from citric acid and ethylenediamine as sources of carbon and nitrogen respectively.…”

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confidence: 99%

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Development of a mycolic acid‐graphene quantum dot probe as a potential tuberculosis biosensor

et al. 2022

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The development of amine‐functionalized graphene quantum dots (GQDs) linked to mycolic acids (MAs) as a potential fluorescent biosensor to detect tuberculosis (TB) biomarkers is described. GQDs have attractive properties: high fluorescence, excellent biocompatibility, good water solubility, and low toxicity. MAs are lipids that are found in the cell wall of Mycobacterium tuberculosis that are antigenic, however, they are soluble only in chloroform and hexane. Chloroform‐soluble MAs were covalently linked to synthesized water‐soluble GQDs using an amide connection to create a potential fluorescent water‐soluble TB biosensor: MA‐GQDs. Fluorescence results showed that GQDs had a narrow emission spectrum with the highest emission at 440 nm, while MA‐GQDs had a broader spectrum with the highest emission at 470 nm, after exciting at 360 nm. The appearance of the peptide bond (amide linkage) in the Fourier‐transform infrared spectrum of MA‐GQDs confirmed the successful linking of MAs to GQDs. Powder X‐ray diffraction exhibited an increase in the number of peaks for MA‐GQDs relative to GQDs, suggesting that linking MAs to GQDs changed the crystal structure thereof. The linked MA‐GQDs showed good solubility in water, high fluorescence, and visual flow through a nitrocellulose membrane. These properties are promising for biomedical fluorescence sensing applications.

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Section: Resultsmentioning

confidence: 99%

Section: Fluorescent Properties Of Gqds and Ma-gqdsmentioning

confidence: 99%

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confidence: 99%

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Development of a mycolic acid‐graphene quantum dot probe as a potential tuberculosis biosensor

et al. 2022

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Quantum Dot-Based Nanosensors for In Vitro Detection of Mycobacterium tuberculosis

Nikolaev,

Lepekhina,

Alliluev

et al. 2024

Nanomaterials

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Despite the existing effective treatment methods, tuberculosis (TB) is the second most deadly infectious disease, its carriers in the latent and active phases accounting for more than 20% of the world population. An effective method for controlling TB and reducing TB mortality is regular population screening aimed at diagnosing the latent form of TB and taking preventive and curative measures. Numerous methods allow diagnosing TB by directly detecting Mycobacterium tuberculosis (M.tb) biomarkers, including M.tb DNA, proteins, and specific metabolites or antibodies produced by the host immune system in response to M.tb. PCR, ELISA, immunofluorescence and immunochemical analyses, flow cytometry, and other methods allow the detection of M.tb biomarkers or the host immune response to M.tb by recording the optical signal from fluorescent or colorimetric dyes that are components of the diagnostic systems. Current research in biosensors is aimed at increasing the sensitivity of detection, a promising approach being the use of fluorescent quantum dots as brighter and more photostable optical tags. Here, we review current methods for the detection of M.tb biomarkers using quantum dot-based nanosensors and summarize data on the M.tb biomarkers whose detection can be made considerably more sensitive by using these sensors.

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Application of Quantum Dots in Lateral Flow Immunoassays: Non-Communicable and Communicable Diseases

Tsolekile¹,

Mngcutsha²,

Vitshima³

2023

Quantum Dots - Recent Advances, New Perspectives and Contemporary Applications

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Quantum Dots (QDs) are ideal fluorescent labels for lateral flow assays (LFA) due to their unique optical properties and resistance to chemical and photo-degradation. Lateral flow assay, known as immunochromatography test, is a diagnostic strip test that uses paper or membrane-based devices to detect the presence/absence of an analyte with the pregnancy test, the most known LFA. Diagnostic tools for non-communicable (NCDs) and communicable diseases (CDs) are available. However, other NCDs and CDs do not have LFAs as first-line diagnosis/point-of-care. QDs are promising fluorescent labels as they improve the LFA’s colourimetric sensitivity and fluorescence stability. This chapter discusses the tailoring and application of QDs in LFA. Particular focus on the applications of QDs in LFA for detecting or screening NCDs (i.e. cancers etc.) and CDs (i.e. SARS-CoV-2, HIV/AIDs etc.).The book chapter concludes by discussing different challenges and perspectives of QDs in LFA.

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Synthesis and characterisation of quantum dots coupled to mycolic acids as a water‐soluble fluorescent probe for potential lateral flow detection of antibodies and diagnosis of tuberculosis

Cited by 3 publications

References 31 publications

Development of a mycolic acid‐graphene quantum dot probe as a potential tuberculosis biosensor

Development of a mycolic acid‐graphene quantum dot probe as a potential tuberculosis biosensor

Quantum Dot-Based Nanosensors for In Vitro Detection of Mycobacterium tuberculosis

Application of Quantum Dots in Lateral Flow Immunoassays: Non-Communicable and Communicable Diseases

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