Synthesis and characterization of a novel multi-functionalized reduced graphene oxide as a pH-sensitive drug delivery material and a photothermal candidate

Mirza‐Aghayan, Maryam; Heidarian, Maryam; Mohammadi, Marzieh; Boukherroub, Rabah

doi:10.1016/j.apsusc.2022.152568

Cited by 17 publications

(4 citation statements)

References 72 publications

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“…These values are close to those determined in previous works for the conjugation of BUFII to magnetite nanoparticles ( Cuellar et al, 2018 ; Perez et al, 2019 ; Ramírez-Acosta et al, 2020 ). The morphology of the nanobioconjugates was effectively determined via microscopic analyses, showing smooth nanosheets as previously reported by authors following similar functionalization approaches to develop drug delivery systems ( Chen et al, 2021 ; Mirza-Aghayan et al, 2022 ). The thicknesses reported in this study (less than 3 nm) are in the range of previous reports for graphene layers ( Chen et al, 2021 ; Mirza-Aghayan et al, 2022 ; Molaparast et al, 2022 ).…”

Section: Discussionmentioning

confidence: 69%

Assessing cellular internalization and endosomal escape abilities of novel BUFII-Graphene oxide nanobioconjugates

et al. 2022

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Cell-penetrating agents based on functionalized nanoplatforms have emerged as a promising approach for developing more efficient and multifunctional delivery vehicles for treating various complex diseases that require reaching different intracellular compartments. Our previous work has shown that achieving full cellular coverage and high endosomal escape rates is possible by interfacing magnetite nanoparticles with potent translocating peptides such as Buforin II (BUF-II). In this work, we extended such an approach to two graphene oxide (GO)-based nanoplatforms functionalized with different surface chemistries to which the peptide molecules were successfully conjugated. The developed nanobioconjugates were characterized via spectroscopic (FTIR, Raman), thermogravimetric, and microscopic (SEM, TEM, and AFM) techniques. Moreover, biocompatibility was assessed via standardized hemocompatibility and cytotoxicity assays in two cell lines. Finally, cell internalization and coverage and endosomal escape abilities were estimated with the aid of confocal microscopy analysis of colocalization of the nanobioconjugates with Lysotracker Green®. Our findings showed coverage values that approached 100% for both cell lines, high biocompatibility, and endosomal escape levels ranging from 30 to 45% and 12–24% for Vero and THP-1 cell lines. This work provides the first routes toward developing the next-generation, carbon-based, cell-penetrating nanovehicles to deliver therapeutic agents. Further studies will be focused on elucidating the intracellular trafficking pathways of the nanobioconjugates to reach different cellular compartments.

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Section: Discussionmentioning

confidence: 69%

Assessing cellular internalization and endosomal escape abilities of novel BUFII-Graphene oxide nanobioconjugates

et al. 2022

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“…Most recently, Mirza-Aghayan et al 240 developed a novel rGO nanocomplex functionalized with dopamine (DA) and Au NPs for intelligent DOX delivery. This nanocarrier displayed 0.852 mg mg −1 DOX loading capacity and 67% drug release in acidic pH besides good PTT properties, which has endowed it with the potential of being used as a cancer therapeutic agent.…”

Section: Combined Therapiesmentioning

confidence: 99%

Graphene family in cancer therapy: recent progress in cancer gene/drug delivery applications

et al. 2023

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“…21 Based on Mirza-Aghayan et al's study, nanocarriers can enter tumorous cells through endocytosis. 22 Graphene oxide (GO) has hydroxyl (−OH), carbonyl (C�O), alkoxy (C−O−C), carboxylic acid (−COOH), and other functional groups with oxygen based on the literature, will all lead to increasing the cytotoxicity. 16,23 Reduced graphene oxide (rGO) can easily be obtained from GO by thermal, chemical, or UV light reduction that leads to a decrease in the number of Ocontaining functional groups.…”

Section: Introductionmentioning

confidence: 99%

“…Among all nanocarriers, carbon-based ones have the most frequent usage in the delivery field due to their distinctive features like binding to the cell membrane, which prepares the best place for DOX release, and also this binding can induce cell membrane ruffling that can stimulate cell stress, which leads to apoptosis, methuosis, and generation of ROS . Based on Mirza-Aghayan et al’s study, nanocarriers can enter tumorous cells through endocytosis . Graphene oxide (GO) has hydroxyl (−OH), carbonyl (CO), alkoxy (C–O–C), carboxylic acid (−COOH), and other functional groups with oxygen based on the literature, will all lead to increasing the cytotoxicity. , Reduced graphene oxide (rGO) can easily be obtained from GO by thermal, chemical, or UV light reduction that leads to a decrease in the number of O-containing functional groups.…”

Section: Introductionmentioning

confidence: 99%

Bioengineered Smart Nanocarriers for Breast Cancer Treatment: Adorned Carbon-Based Nanocomposites with Silver and Palladium Complexes for Efficient Drug Delivery

Safarkhani,

Moghaddam,

Taghavimandi

et al. 2023

ACS Omega

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Biocompatible and bioactive carbon-based nanocomposites are ingeniously designed and fabricated with the aim of enhancing drug delivery applicability in breast cancer treatment. Reduced graphene oxide (rGO) and multiwalled carbon nanotubes (MWCNTs) are utilized as nanocarriers for increasing penetrability into cells and the loading capacity. What sets our study apart is the strategic incorporation of the two different complexes of silver (AgL2) and palladium (PdL2) with the carboxamide-based ligand C9H7N3OS (L), which have been synthesized and decorated on nanocarriers alongside doxorubicin (DOX) for stabilizing DOX by π–π interactions and hydrogen bonding. Although DOX is a well-known cancer therapy agent, the efficacy of DOX is hindered owing to drug resistance, poor internalization, and limited site specificity. Aside from stabilizing DOX on nanocarriers, our carbon-based nanocarriers are tailored to act as a precision-guided missile, strategically by adorning with target-sensitive complexes. Based on the literature, carboxamide ligands can connect to overexpressed receptors on cancerous cells and inhibit them from proliferation signaling. Also, the complexes have an antibacterial activity that can control the infection caused by decreasing white blood cells and necrosis of cancerous cells. A high-concentration cytotoxicity assay revealed that decorating PdL2 on a DOX-containing nanocarrier not only increased cytotoxicity to breast cancerous cell lines (MDA-MB-231 and MCF-7) but also revealed higher cell viability on a normal cell line (MCF-10A). The drug release screening results showed that the presence of PdL2 led to 72 h correlate release behavior in acidic and physiological pH profiles, while the AgL2‑containing nanocomposite showed an analogue behavior for just 6 h and the release of DOX continued and after about 100 h hit the top.

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Synthesis and characterization of a novel multi-functionalized reduced graphene oxide as a pH-sensitive drug delivery material and a photothermal candidate

Cited by 17 publications

References 72 publications

Assessing cellular internalization and endosomal escape abilities of novel BUFII-Graphene oxide nanobioconjugates

Assessing cellular internalization and endosomal escape abilities of novel BUFII-Graphene oxide nanobioconjugates

Graphene family in cancer therapy: recent progress in cancer gene/drug delivery applications

Bioengineered Smart Nanocarriers for Breast Cancer Treatment: Adorned Carbon-Based Nanocomposites with Silver and Palladium Complexes for Efficient Drug Delivery

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