Synthesis and characterization of a novel magnetic chitosan microsphere for lactase immobilization

Ke, Ping; Xu, Ke; Cui, Jianlei; Li, Xin; Wang, Guanghui

doi:10.1016/j.colsurfa.2020.125522

Cited by 22 publications

(8 citation statements)

References 30 publications

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“…The Michaelis constant Km is considered the most important constant for studying the kinetics of the enzyme reaction, which represents the affinity between the enzyme and the substrate. Larger Km values indicated weaker affinity and vice versa [ 46 ]. The Km of the co-immobilized enzymes was determined by using a series of concentrations of the lactose/fructose mixture (10% lactose and 2% fructose) as the substrate according to the Michaelis–Menten kinetics.…”

Section: Resultsmentioning

confidence: 99%

“…Lactulose synthesis with biological enzymes could overcome some disadvantages of industrial chemical synthesis, such as the degradation of lactulose, side reaction, purification of late products, and so on [ 45 ]. Lactose can be converted into galactose and glucose under the catalysis of lactase [ 46 ]. Glucose was isomerized to fructose by glucose isomerase, then fructose and galactose were linked by a β-1, 4 glycosidic bond to synthesize lactulose under the catalysis of lactase [ 47 , 48 ].…”

Section: Introductionmentioning

confidence: 99%

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Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

et al. 2022

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The g-C3N4/CF composite carrier was prepared by ultrasound-assisted maceration and high-temperature calcination. The enzyme immobilization using the g-C3N4/CF as the novel carrier to immobilize lactase and glucose isomerase was enhanced for lactulose production. The carbon fiber (CF) was mixed with melamine powder in the mass ratio of 1:8. The g-C3N4/CF composite carrier was obtained by calcination at 550 °C for 3 h. After the analysis of characteristics, the g-C3N4/CF was successfully composited with the carbon nitride and CF, displaying the improvement of co-immobilization efficiency with the positive effects on the stability of the enzyme. The immobilization efficiency of the co-immobilized enzyme was 37% by the novel carrier of g-C3N4/CF, with the enzyme activity of 13.89 U g−1 at 60 °C. The relative activities of co-immobilized enzymes maintained much more steadily at the wider pH and higher temperature than those of the free dual enzymes, respectively. In the multi-batches of lactulose production, the relative conversion rates in enzymes co-immobilized by the composite carrier were higher than that of the free enzymes during the first four batches, as well as maintaining about a 90% relative conversation rate after the sixth batch. This study provides a novel method for the application of g-C3N4/CF in the field of immobilizing enzymes for the production of lactulose.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

et al. 2022

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“…Ke et al created a novel magnetic chitosan microsphere with Fe 3 O 4 as the enzyme support. The immobilized lactase by this material has better pH and thermal stability and it also showed higher activity than the free enzyme [72]. In their study, immobilized enzyme reached the highest activity at pH = 8, while the free enzyme only could remain about 20% of its activity [72].…”

Section: Applications In Foodmentioning

confidence: 93%

“…The immobilized lactase by this material has better pH and thermal stability and it also showed higher activity than the free enzyme [72]. In their study, immobilized enzyme reached the highest activity at pH = 8, while the free enzyme only could remain about 20% of its activity [72]. In addition, dairy products, juices are also popular items in the markets, the immobilized enzymes are also used wildly in this area.…”

Section: Applications In Foodmentioning

confidence: 96%

Immobilization of Enzymes by Polymeric Materials

et al. 2021

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Enzymes are the highly efficient biocatalyst in modern biotechnological industries. Due to the fragile property exposed to the external stimulus, the application of enzymes is highly limited. The immobilized enzyme by polymer has become a research hotspot to empower enzymes with more extraordinary properties and broader usage. Compared with free enzyme, polymer immobilized enzymes improve thermal and operational stability in harsh environments, such as extreme pH, temperature and concentration. Furthermore, good reusability is also highly expected. The first part of this study reviews the three primary immobilization methods: physical adsorption, covalent binding and entrapment, with their advantages and drawbacks. The second part of this paper includes some polymer applications and their derivatives in the immobilization of enzymes.

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“…Another strategy for addressing the issue of enzyme consumption resides in postprocess enzyme recovery, i.e., enzyme separation after its use in a given process. Possible approaches in this regard involved enzyme immobilization on solids as already reported by the fairly extensive literature [64][65][66][67][68][69][70][71][72][73][74], and many works were devoted to food technology purposes [72,73,[75][76][77][78][79][80][81]. The use of immobilized enzymes such as lactase for continuous hydrolysis of lactose in milk for the production of lactose-free milk is one of the most important applications in dairy technology [82].…”

Section: Post-process Strategies For Enzyme Recoverymentioning

confidence: 99%

Role of Clay Substrate Molecular Interactions in Some Dairy Technology Applications

Azzouz,

Arus,

Platon

2024

IJMS

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The use of clay materials in dairy technology requires a multidisciplinary approach that allows correlating clay efficiency in the targeted application to its interactions with milk components. For profitability reasons, natural clays and clay minerals can be used as low-cost and harmless food-compatible materials for improving key processes such as fermentation and coagulation. Under chemical stability conditions, clay materials can act as adsorbents, since anionic clay minerals such as hydrotalcite already showed effectiveness in the continuous removal of lactic acid via in situ anion exchange during fermentation and ex situ regeneration by ozone. Raw and modified bentonites and smectites have also been used as adsorbents in aflatoxin retention and as acidic species in milk acidification and coagulation. Aflatoxins and organophilic milk components, particularly non-charged caseins around their isoelectric points, are expected to display high affinity towards high silica regions on the clay surface. Here, clay interactions with milk components are key factors that govern adsorption and surface physicochemical processes. Knowledge about these interactions and changes in clay behavior according to the pH and chemical composition of the liquid media and, more importantly, clay chemical stability is an essential requirement for understanding process improvements in dairy technology, both upstream and downstream of milk production. The present paper provides a comprehensive review with deep analysis and synthesis of the main findings of studies in this area. This may be greatly useful for mastering milk processing efficiency and envisaging new prospects in dairy technology.

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Synthesis and characterization of a novel magnetic chitosan microsphere for lactase immobilization

Cited by 22 publications

References 30 publications

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

Co-Immobilization of Lactase and Glucose Isomerase on the Novel g-C3N4/CF Composite Carrier for Lactulose Production

Immobilization of Enzymes by Polymeric Materials

Role of Clay Substrate Molecular Interactions in Some Dairy Technology Applications

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