Synthesis and characterization of collagen‐chitosan‐hydroxyapatite artificial bone matrix

Abstract

In this study, a new artificial bone matrix was constructed with collagen and Ca(5)(PO(4))(3)OH (hydroxyapatite/HA) which are the main components of natural bone. To improve the property of the artificial bone matrix, chitosan (CS), a kind of positive charged polysaccharide, was crosslinked into the scaffolds. Solid-liquid phase separation method was used to shape 3D porous structure benefited for cells growing into. The artificial bone matrix was investigated by transmission electron microscopy, scanning electron microscopy, and electron spectroscopy for chemical analysis, etc. for structures and characteristics. And its ability of bone repair was investigated by orthotope bone defect reparation in vivo. The results showed that the artificial bone matrix was a porousscaffold with three-dimension interconnected fiber microstructure. HA particles were dispersed evenly among collagen fiber and CS was modified on the surface of collagen fiber. It was indicated that this artificial bone matrix could be used as a bone substitute with outstanding properties.

Biocomposites containing natural polymers and hydroxyapatite for bone tissue engineering

“…Zhao et al [38] fabricated two types of biomimetic composite materials, chitosan-gelatin (CG) and hydroxyapatite/chitosan-gelatin (HCG) and demonstrated enhanced protein and calcium ion adsorption properties of HAp in the CG polymer network which improved initial cell-adhesion and long-term growth, favour osteogenic differentiation upon induction, as well as maintained the progenicity of the 3D human mesenchymal stem cell constructs. A new artificial bone matrix with collagen and Ca(5)(PO(4))(3)OH (hydroxyapatite/HAp) which are the main components of natural bone was prepared [39]. To improve the property of the artificial bone matrix, chitosan was cross-linked into the scaffolds.…”

Structure and mechanical properties of supercritical carbon dioxide processed porous resorbable polymer constructs

“…Phase separation processes, which involve the dissolution of a polymer in a volatile organic solvent and subsequent induction of a thermodynamic instability (i.e. rapid changes in temperature and/or pressure), have been used with success (Hu et al, 2001;Nam and Park, 1999;Wang et al, 2008). Wang et al recently reported success in using a collagen/PLA/hydroxyapatite composite fabricated by a freeze-drying method in the healing of a 15 mm segmental defect in the rabbit radius.…”

“…Numerous techniques have been explored in tissue engineering literature, which seek to synthesize resorbable polymer constructs with porous structures (Georgiou et al, 2007;Hu et al, 2001;Kim et al, 2007;Mathieu et al, 2005;Nam and Park, 1999;Teng et al, 2007;Wang et al, 2008;Zeng et al, 2003). Phase separation processes, which involve the dissolution of a polymer in a volatile organic solvent and subsequent induction of a thermodynamic instability (i.e.…”

Biomimetic Collagen Nanofibrous Materials for Bone Tissue Engineering

“…Their data suggest that porous collagen/HA scaffolds are promising candidates for application as bone grafts to improve the property of the collagen matrix. Wang and co-workers [170] crosslinked chitosan, a positive charged polysaccharide, into the scaffolds using solid-liquid phase separation. The ability of the porous collagen/chitosan scaffold to repair bone was investigated by orthotope bone defect reparation in vivo.…”