Synthesis and Characterization of Curcumin-Chitosan Loaded Gold Nanoparticles by Oryctes rhinoceros’ Chitin for Cosmeceutical Application

Abstract

A breakthrough in cosmeceuticals by utilizing insects as major ingredients in cosmetic products is gaining popularity. Therefore, the interest in rare sources of ingredients, for instance, from the Oryctes rhinoceros beetle, can bring huge benefits in terms of turning pests into wealth. In this study, curcumin was chosen as the active ingredient loaded into chitosan-gold nanoparticles (CCG-NP). Curcumin is unstable and has poor absorption, a high rate of metabolism, and high sensitivity to light. These are all factors that contribute to the low bioavailability of any substance to reach the target cells. Therefore, chitosan extracted from O. rhinoceros could be used as a drug carrier to overcome these limitations. In order to overcome these limitations, CCG-NPs were synthesized and characterized. Chitosan was isolated from O. rhinoceros and CCG-NPs were successfully synthesized at 70 °C for 60 min under optimal conditions of a reactant ratio of 2:0.5 (0.5 mM HAuCl4: 0.1% curcumin). Characterizations of CCG-NP involved FTIR analysis, zeta potential, morphological properties determination by FE-SEM, particle size analysis, crystallinity study by XRD, and elemental analysis by EDX. The shape of the CCG-NP was round, its size was 128.27 d.nm, and the value of the zeta potential was 20.2 ± 3.81 mV. The IC50 value for cell viability is 58%, indicating a mild toxicity trait. To conclude, CCG-NP is a stable, spherical, nano-sized, non-toxic, and homogeneous solution.

Improvement of Chitosan Films Properties by Blending with Cellulose, Honey and Curcumin

“…The peak at 2θ = 17°, which was shifted to higher peak positions of 18.2° and 18.5° for both Cur concentrations, respectively, and which are more sharp, was due to curcumin’s crystalline form. The broad peak of (2θ = 22.3°) was slightly shifted to a lower peak position at 21.1° and 21.5° in both concentrations of curcumin, respectively, with decreasing intensity and broadening of the peak according to the pure CS, while the other peak increased in intensity [ 9 , 33 , 34 ].…”

“…Some peaks of Cur seem to be absent in the spectrum of the blends due to the formation of an amorphous complex with the intermolecular interaction occurring within CS and Cur molecules. This suggests a good diffusion of curcumin inside the CS matrix [ 33 , 34 , 35 ].…”

Green synthesis of gold nanoparticles using macroalgae Halimeda macroloba extract and their photocatalytic degradation of methylene blue and methyl orange

“…Nanotechnology usually comprises nanoparticles ranging from 1 to 100 nm 9 . Metal oxide nanoparticles have attracted considerable interest in recent years due to the availability of numerous applications that benefit from their enormous surface area, band gap stability, and reusability 10,11 . Because of their various flexible features, metal nanoparticles have drawn the interest of researchers.…”

“…9 Metal oxide nanoparticles have attracted considerable interest in recent years due to the availability of numerous applications that benefit from their enormous surface area, band gap stability, and reusability. 10,11 Because of their various flexible features, metal nanoparticles have drawn the interest of researchers. Various methods exist for creating metal nanoparticles, including chemical and physical approaches, each with its own limits.…”