Synthesis and Characterization of Ferrous and Copper Nanoparticles from E-Waste Using Biological Reduction by Lichen-Associated Bacteria and Their Application in Antifouling Activity

Subbaiyan, Rubavathi; Ganesan, Ayyappadasan; Sasikumar, Bhurniammal; Rajendran, Srilekha; Brindha, R.

doi:10.1007/s12010-022-04293-w

Cited by 12 publications

(12 citation statements)

References 31 publications

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“…Many studies also suggest the presence of other antibacterial lipopeptides such as iturin ( R f = 0.3) and fengycin ( R f = 0.09) in the culture extracts of B. subtilis 31 . Nanomaterials from lichen‐associated bacteria, a technology boon, provide a variety of methods for improving the effectiveness of the inhibition of the microorganism to which they are inoculated 32 , 33 …”

Section: Resultsmentioning

confidence: 99%

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Modeling and optimization of antibacterial effect of lichen‐associated bacteria, Bacillus subtilis KSRLAB3 strain against marine fouling bacteria, Vibrio alginolyticus

Subbaiyan¹,

Ganesan²

2023

Biotech and App Biochem

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One of the most commonly occurring bacteria, Bacillus subtilis, can produce a wide variety of secondary metabolites. In this study, the antimicrobial effect of B. subtilis KSRLAB3 against Vibrio alginolyticus was optimized using the Plackett–Burman design (PBD) method, response surface methodology (RSM), and genetic algorithm (GA). Initially, the effects of carbon source, nitrogen source, NaCl concentration, pH, temperature, and incubation time on antimicrobial effects were studied. Among the carbon and nitrogen sources investigated, mannose and peptone elicited maximum antimicrobial effect. Using PBD, the most significant variables that influence the antimicrobial effect were identified, including incubation time, peptone concentration, and temperature. The optimum conditions required for attaining maximum antimicrobial effect was identified using the RSM‐GA hybrid method, and the optimum condition includes 49.999 h of incubation time, 4.39 g/L of peptone concentration, and 27.629°C of incubation temperature. The confirmatory experiments performed around the optimum condition showed a zone of inhibition of 35 ± 0.52 mm. Methanolic extract also proved the presence of antibacterial lipopeptide surfactin. Therefore, the RSM‐GA hybrid method was successfully used in this study to model the antimicrobial effect of B. subtilis KSRLAB3 against V. alginolyticus. The effective inhibition of V. alginolyticus can be investigated further for the development of antifouling coatings.

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Section: Resultsmentioning

confidence: 99%

“…31 Nanomaterials from lichenassociated bacteria, a technology boon, provide a variety of methods for improving the effectiveness of the inhibition of the microorganism to which they are inoculated. 32,33…”

Section: Characterization Of Antibacterial Extract From B Subtilis Ks...mentioning

confidence: 99%

Modeling and optimization of antibacterial effect of lichen‐associated bacteria, Bacillus subtilis KSRLAB3 strain against marine fouling bacteria, Vibrio alginolyticus

Subbaiyan¹,

Ganesan²

2023

Biotech and App Biochem

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“…One milliliters aliquot of each dilution sample was added to the Petri plates consisting of Zobell marine agar medium. These Petri plates were then incubated overnight, and bacterial colonies were observed in Petri plates after 24–48 h. The pure bacterial colonies were separated using quadrant streaking methods and incubated for about 48 h at the incubation temperature of 37°C 15,16 …”

Section: Methodsmentioning

confidence: 99%

“…The synthesized Zn NPs were characterized using XRD technique, UV spectrophotometer, FTIR, and SEM coupled with EDAX. 16…”

Section: Production Of Zinc Nanoparticles and Its Characterizationmentioning

confidence: 99%

Self potent antimicrobial and antifouling properties of zinc nanoparticles derived from lichen symbionts

Subbaiyan,

Ganesan,

Muthusamy

et al. 2023

Env Prog and Sustain Energy

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Biofouling poses a significant economic challenge for marine transportation. The use of chemical compounds to eliminate fouling layers beneath the ship's surface, albeit effective, raises concerns due to its potential harm to the aquatic ecosystem. Lichen symbiotic associations, an underexplored research area, show promise in producing antimicrobial and antifouling compounds. These lichen‐associated bacteria engage in various functional activities and have multiple bacterial counterparts. The optimal conditions for cultivating lichen‐associated bacteria for maximum growth were identified as a pH of 7, a temperature of 30°C, and an incubation period of 8 days. Glucose served as the carbon source, while yeast extract served as the nitrogen source. Additionally, zinc nanoparticles were successfully synthesized from the thriving lichen‐associated bacteria. Fourier transform infrared spectroscopy (FTIR) analysis indicated distinctive bands at 3890 and 3103 cm−1, corresponding to the OH stretching of alcohol and acid stretch bands, respectively. Furthermore, the presence of amide in NH banding in the synthesized zinc nanoparticles was represented by a band at 1636 cm−1. SEM images of the produced zinc NPs displayed a spherical shape, ranging in size from 40 to 70 nm. To assess the impact of these nanoparticles from lichen‐associated bacteria on biofouling activity in marine transportation, zebrafish results were analyzed.

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“…Later, proteins from microfoulers act as a signal to macrofoulers and organisms of other species, most commonly barnacles, mussels, and so on. This macrofouling increases a load of marine equipment, resulting in a rise in fuel consumption, material degradation, decreased performance, and increased maintenance costs 3,4 . Barnacle attaches to marine equipment underwater by secreting an adhesive called adhesive proteins.…”

Section: Introductionmentioning

confidence: 99%

Virtual screening of the natural antifoulants: In silico approach to screen lichen metabolites against marine biofoulers

Subbaiyan,

Ganesan,

Dhanuskodi

et al. 2023

Env Prog and Sustain Energy

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The present study aims to explore the inhibitory action of lichen metabolites against important protein targets of marine fouling organisms using computational methods. Lichens with their unique ability to produce various secondary metabolites find versatile applications in Biotechnology and allied fields. The 3D structure of target proteins associated with fouling activity of marine organism were collected from the protein data bank (PDB) and the computational docking analysis was performed using the PyRx software with 40 lichen compounds that are collected from PubChem. The lichen compounds were docked against the following protein targets—mannose‐specific lectin CGL1 of Crassostrea gigas, Proximal thread matrix protein 1 PTMP 1 of Mytilus galloprovincialis, and G protein‐coupled receptor (GPCR) of Balanus amphitrite. As there was no experimentally determined structure available for GPCR of B. amphitrite, homology modeling was performed to predict the protein structure. The docking study showed effective binding of lichen compounds to various fouling targets with the binding energies in the range of −10.1 to −5 kcal/mol. Among the lichen compounds studied, roccanin showed the least binding energy for CGL1, PTMP1 and GPCR. The correlation assessments predict a positive relationship between binding energy, molecular weight and retention time. MD Simulation studies were performed using LARMD online tool and the RMSD and radius of gyration (Rg) values was recorded. This study proves that the compounds produced by lichens have high antifouling effect against marine foulers under in silico conditions.

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Synthesis and Characterization of Ferrous and Copper Nanoparticles from E-Waste Using Biological Reduction by Lichen-Associated Bacteria and Their Application in Antifouling Activity

Cited by 12 publications

References 31 publications

Modeling and optimization of antibacterial effect of lichen‐associated bacteria, Bacillus subtilis KSRLAB3 strain against marine fouling bacteria, Vibrio alginolyticus

Modeling and optimization of antibacterial effect of lichen‐associated bacteria, Bacillus subtilis KSRLAB3 strain against marine fouling bacteria, Vibrio alginolyticus

Self potent antimicrobial and antifouling properties of zinc nanoparticles derived from lichen symbionts

Virtual screening of the natural antifoulants: In silico approach to screen lichen metabolites against marine biofoulers

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