Synthesis and characterization of HPMA copolymer–aminopropylgeldanamycin conjugates

Kasuya, Yuko; Zhang, Lu; Kopečková, Pavla; Minko, Tamara; Tabibi, S. Esmail; Kopeček, Jindřich

doi:10.1016/s0168-3659(01)00318-2

Cited by 48 publications

(29 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…GDM-OH was synthesized by a method reported previously with slight modification (25). GDM was first obtained by fermentation of Streptomyces hygroscopicus subsp.…”

Section: Methodsmentioning

confidence: 99%

Mixed pH-Sensitive Polymeric Micelles for Combination Drug Delivery

et al. 2010

View full text Add to dashboard Cite

Purpose To prepare mixed polymeric micelles that can carry two different drugs, doxorubicin (DOX) and 17-hydroxyethylamino-17-demethoxygeldanamycin (GDM-OH), for combination cancer chemotherapy. Methods The pH-sensitive micelles were prepared from poly (ethylene glycol)-poly(aspartate hydrazide) block copolymers to which either DOX or GDM-OH is conjugated through acid-labile hydrazone bond (individual micelles). Mixed micelles were formed not only by simply mixing two different individual micelles in aqueous solutions (aqueous mixed micelles) but also by evaporating organic solvents from the organic/aqueous mixed solvents in which two block copolymers possessing different drugs were dissolved homogeneously (organic mixed micelles). Particle size measurements, pH-dependent drug release tests, cytotoxicity assays and western blot analysis were subsequently conducted. Results Individual and aqueous/organic mixed micelles showed clinically relevant particle size (<100 nm) and pH-dependent drug release patterns. Mixed polymer micelles suppress cancer cell growth effectively in a drug concentration, mixing method and schedule-dependent way. Conclusion Combination chemotherapy using polymeric micelles seems to minimize a schedule-dependent change in combination drug efficacy in comparison to drug combination using DMSO formulations.

show abstract

“…GDM-OH was synthesized by a method reported previously with slight modification (25). GDM was first obtained by fermentation of Streptomyces hygroscopicus subsp.…”

Section: Methodsmentioning

confidence: 99%

Mixed pH-Sensitive Polymeric Micelles for Combination Drug Delivery

et al. 2010

View full text Add to dashboard Cite

show abstract

“…17-(6-aminohexylamino)-17-demethoxygeldanamycin (AH-GDM) and N-methacryloylglycylphenylalanylleucylglycl-17-(6-aminohexylamino)-17-demethoxygeldanamycin (MA-GFLG-AH-GDM) were synthesized according to previously described procedures with minor modifications (15,39). Briefly, GDM (200 mg, 0.357 mmol) and 1,6-Diaminohexane (1.24 g, 10.7 mmol) were dissolved in anhydrous chloroform (30 mL) and stirred under N 2 gas for 2 h at room temperature.…”

Section: Synthesis and Characterization Of Drug-containing Comonomersmentioning

confidence: 99%

HPMA Copolymer-Aminohexylgeldanamycin Conjugates Targeting Cell Surface Expressed GRP78 in Prostate Cancer

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Kopecek's group synthesized a non-toxic and non-immunogenic N-(2-hydroxypropy1) methacrylamide (HPMA) copolymer grafted with anticancer drugs (e.g., DOX, mesochlorin e6 monoethylenediamine, geldanamycin (GDM), gemcitabine) via a lysosomally-degradable tetrapeptide linker (Gly-Phe-leu-Gly, GFLG) [60][61][62][63][64][65][66][67][68][69][70][71][72]. Here, GFLG is a specific area that can be cleaved by a lysosomal enzymatic system, such as cathepsin B and cysteine proteinase.…”

Section: Enzyme-activating Systemsmentioning

confidence: 99%

Intelligent Polymeric Nanocarriers Responding to Physical or Biological Signals: A New Paradigm of Cytosolic Drug Delivery for Tumor Treatment

Lee

Baik

et al. 2010

Polymers

View full text Add to dashboard Cite

Abstract:The physicochemical properties of stimuli-responsive polymers change with physical or biological signals, such as pH, enzyme concentrations, and temperature. These polymers have attracted considerable attention in the field of drug delivery. The drug carrier system, which was revolutionized by the introduction of these polymers, has recently provided a new paradigm of maximizing the therapeutic activity of drugs. This review highlights recent studies regarding stimuli-responsive drug carriers tailor-made for effective cytosolic drug delivery, with particular emphasis on tumor treatment.

show abstract

Synthesis and characterization of HPMA copolymer–aminopropylgeldanamycin conjugates

Cited by 48 publications

References 33 publications

Mixed pH-Sensitive Polymeric Micelles for Combination Drug Delivery

Mixed pH-Sensitive Polymeric Micelles for Combination Drug Delivery

HPMA Copolymer-Aminohexylgeldanamycin Conjugates Targeting Cell Surface Expressed GRP78 in Prostate Cancer

Intelligent Polymeric Nanocarriers Responding to Physical or Biological Signals: A New Paradigm of Cytosolic Drug Delivery for Tumor Treatment

Contact Info

Product

Resources

About