Synthesis and characterization of mesoporous Cu-MOF for laccase immobilization

Abstract

BACKGROUND Mesoporous metal–organic frameworks (MOFs) have been widely researched for enzyme immobilization, however, most are unstable in the aqueous phase. This paper reports the synthesis of a Cu–MOF for the immobilization of copper contained laccase via a surfactant‐templating method with various surfactant/chelating agent mass ratios. RESULTS The Brunauer–Emmett–Teller (BET) surface area and pore diameter of the nanoscale Cu–MOF were 366–1154 m2 g−1 and 15.2–20.7 nm, respectively. The adsorption concentration and time were optimized to be 5 mg laccase mL−1 and 1 h. This immobilized system exhibited adsorption capacity up to 502 mg g−1 and activity recovery rate of 95.2%. The optimum reaction conditions for immobilizing laccase on Cu–MOF were pH 4 and 50°C. According to the Lineweaver–Burk plot, the Km of the immobilized laccase was 0.157 mmol L−1, indicating that the affinity of immobilized laccase to its substrate was higher than that of free laccase (0.290 mmol L−1). The activity of immobilized laccase after seven iterations of reuse remained approximately 50%. CONCLUSION The mesoporous Cu–MOF showed favorable adsorption capability and can be utilized in laccase immobilization. © 2017 Society of Chemical Industry

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