Synthesis and characterization of new branched magnetic nanocomposite for loading and release of topotecan anti-cancer drug

Abstract

The purpose of this study is to synthesize the new branched magnetic nanocomposite to carry the therapeutic agents and bio-macromolecules. Although topotecan (TPT) has significant antitumor activity to lung, ovarian, breast, pancreas, and stomach cancers, lactone ring opening causes a reduction in cytotoxic activity and severe side effects in physiological conditions. In order to contribute to the removal of the handicap (lactone ring opening), magnetic dextran branched with NαNα-Bis (carboxymethyl)-L-lysine hydrate (NTA) nanoparticles (MD 3) were prepared. The characterization of the resulting MD 3 material was done by using multi-pronged analyses. The stability and release of TPT with synthesized and characterized new material of the MD 3 was studied using various essential factors like concentration, dosage, pH, and time. The entrapment efficiency and loading capacity of TPT onto MD 3 was calculated as 32.2% and 1.44 mg/g, respectively, at pH 5. Release studies were performed with drug-loaded MD 3 at different pH values. It was seen that the best release was obtained at the cancerous site pH. Initial drug concentration was measured as 0.118 mM. The loaded drug concentration was calculated as 0.0380 mM at pH 5 and 0.00092 mM of drug was released after 90 min at pH 5.8. Percentage released of drug was found as 2.42% during 90 min.

Synthesis and characterization of magnetic nanocomposite for in vitro evaluation of irinotecan using human cell lines

“…Therefore, some part of the loaded drug is released from the surface when the interaction time was increased. As a matter of fact, in our previous study, we observed a similar situation in topotecan loading on nanocomposite [2]. The results were shown in Table. According to Table, the drug loading capacity (mg/g) decreased when interaction time between MOCC and CPT-11 was increased.…”

“…The drug loading efficiency (%) decreased when the interaction time between the drug and the surface was increased. This situation might be explained by the fact that the loading of CPT-11 on the MOCC occurs through physical interactions such as the hydrogen bond, van der Waals, and dipole interaction [2]. Therefore, some part of the loaded drug is released from the surface when the interaction time was increased.…”

A Critical Scrutiny on Liposomal Nanoparticles Drug Carriers as Modelled by Topotecan Encapsulation and Release in Treating Cancer

“…One of the major disadvantages of the drug under physiological conditions is that the ring opening of the drug structure results in ineffectiveness, thus affecting drug efficacy [ 27 , 28 ]. To circumvent the aforementioned drawback, Tarhan et al [ 29 ] prepared special nanoparticles, which were referred to as magnetic dextran nanoparticles branched with N α N α -Bis (carboxymethyl)-L-lysine hydrate. Seleci et al [ 30 ] prepared a variety of lipid nanoparticles, including LQ-Dots, which were loaded with TPT.…”

Sintesis Dan Analisis Struktur Kristal Nanopartikel Zno Menggunakan Metode Presipitasi Ph-12

“…Partikel berukuran 1-100 nm biasa disebut dengan nanopartikel [1]. Secara umum nanopartikel memiliki karakteristik ringan dan kecil [2]. Nanopartikel merupakan material penelitian yang sangat menarik, material berukuran nano biasanya memiliki partikel dengan sifat kimia atau fisika yang lebih unggul dari material berukuran besar [3].…”