Synthesis and characterization of V₂O₅/urease for a biosensor of urea

Abstract

Vanadium pentoxide (V2O5) was used as support material to immobilize the urease enzyme. V2O5 was prepared from a sol–gel process, and the immobilization of urease was done onto the surface of the PET/ITO/V2O5 film. Different concentrations of urea were tested to study the sensitivity of the biosensor. Tests with pH variation were carried out, and it was noticed that the total charge in the cyclic voltammograms decreases due to the increase in pH that influences the catalytic activity of the enzyme. From cyclic voltammograms, with a different scan rate, for linear correlation testing, it was observed that the anodic currents varied as a function of the scan rate. Based on the variation in the urea concentration, the current increased with the increase in the urea concentration, indicating the sensitivity of the material. The use of V2O5/urease showed favorable electrochemical responses for application in biosensors as well as being sensitive to the variation in the urea concentration and easy to obtain and prepare.

Vanadium Pentoxide (V2O5): Their Obtaining Methods and Wide Applications

“…Similar to the sensor, a biossensor turn an analytical response into a measurable chemical signal and detects only a certain biological product as a target analyte. The use of different structures of V 2 O 5 as sensitive component in biosensor device has been explored in several target analyte such as urea, glucose, gene sequence, EGFET-biosensor, methylglyoxal in rice and among others [48][49][50][51][52][53].…”