Synthesis and Cze Analysis of Pamam Dendrimers With an Ethylenediamine Core

Characterization of amino-terminated polyamidoamine dendrimers by CE suffers from a lack of resolution for higher generations and poor between-day reproducibility of retention times. Under optimal conditions of temperature, voltage, and sample amount, 0-5 generations of dendrimers could be resolved with a bare fused-silica capillary. However, reproducibility was poor due to potential interactions of the polycationic dendrimers with the uncoated quartz capillary wall. Use of a poly(vinyl alcohol)-coated capillary significantly decreased the migration times of the nanomolecules without compromising resolution. Dendrimer mixtures containing generations 0-5 are separated as discrete, nonoverlapping peaks in about 15 min. In addition, the between-day precision of retention times was dramatically improved without the need for internal standards or data normalization. Dendrimers of various generations and cores run on different days showed an RSD of retention times of less than 4%. The poly(vinyl alcohol) coating was very stable as shown by the excellent precision of migration times obtained on a capillary used for a month with more than 100 injections. Similar to PAGE, separation of polyamidoamine dendrimers on a bare fused-silica and poly(vinyl alcohol)-coated capillary showed an exponential relationship between migration times and calculated charge density of the nanomolecules.

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Statistical Megamer Morphologies and Materials from PAMAM Dendrimers

Khopade¹,

Möhwald²

2005

Macromol. Rapid Commun.

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Summary: Generation‐4 poly(amidoamine) dendrimers (G4‐PAMAM) were cross‐linked using gluteraldehyde to obtain aldehyde‐activated G4‐PAMAM and cross‐linked G4‐PAMAM, termed pre‐megamers, which were grafted with octadecylamine (ODA) in chloroform (CHCl3). Statistical megamer morphologies and particles were prepared by an emulsification solvent‐evaporation method using an ODA‐premegamer/CHCl3 solution, surfactant, and water. Physicochemical characterization of the premegamers and megamers by various spectroscopic and microscopic techniques demonstrated their formation and properties useful in diagnostic and chromatographic applications.Formation of pre‐megamers and megamers and their transmission electron microscopy images.imageFormation of pre‐megamers and megamers and their transmission electron microscopy images.

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Synthesis and Cze Analysis of Pamam Dendrimers With an Ethylenediamine Core

Cited by 29 publications

References 14 publications

Applications of Textile Materials Using Emerging Sources and Technology: A New Perspective

Applications of Textile Materials Using Emerging Sources and Technology: A New Perspective

Use of poly(vinyl alcohol)‐coated capillaries for separation of amino‐terminated polyamidoamine dendrimers

Statistical Megamer Morphologies and Materials from PAMAM Dendrimers

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