Synthesis and degradation of poly (2-hydroxyethyl methacrylate)-graft-poly (ε-caprolactone) copolymers

Cretu, Adina; Gattin, Richard; Brachais, Laurent; Barbier‐Baudry, D.

doi:10.1016/j.polymdegradstab.2003.09.001

Cited by 49 publications

(38 citation statements)

References 20 publications

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“…Higher rates of hydrolysis are observed for poly(caprolactone) with lower molar masses and have been tested in basic and acidic conditions 9 . The rate of hydrolysis of poly(caprolactone) can also be enhanced by the presence and concentration of enzymes, such as lipase 10,11 . Polyurethanes that contain poly(caprolactone) as soft segments commonly present low rates of hydrolysis in neutral pH.…”

Section: Introductionmentioning

confidence: 99%

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

et al. 2010

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A porous biodegradable polyurethane nanocomposite based on poly(caprolactone) (PCL) and nanocomponents derived from montmorillonite (Cloisite ® 30B) was synthesized and tested to produce information regarding its potential use as a scaffold for tissue engineering. Structural and morphological characteristics of this nanocomposite were studied by infrared spectroscopy (FTIR), X-ray diffraction (XRD), small angle X-ray scattering (SAXS) and scanning electron microscopy (SEM). The reaction between polyurethane oligomers with isocyanate endcapped chains and water led to the evolution of CO 2 , which was responsible for building interconnected pores with sizes ranging from 184 to 387 µm. An in vitro cell-nanocomposite interaction study was carried out using neonatal rat calvarial osteoblasts. The ability of cells to proliferate and produce an extracellular matrix in contact with the synthesized material was assessed by an MTT assay, a collagen synthesis analysis, and the expression of alkaline phosphatase. In vivo experiments were performed by subcutaneously implanting samples in the dorsum of rats. The implants were removed after 14, 21, and 29 days, and were analyzed by SEM and optical microscopy after tissue processing. Histology crosssections and SEM analyses showed that the cells were able to penetrate into the material and to attach to many location throughout the pore structure. In vitro and in vivo tests demonstrated the feasibility for polyurethane nanocomposites to be used as artificial extracellular matrices onto which cells can attach, grow, and form new tissues.

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Section: Introductionmentioning

confidence: 99%

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

et al. 2010

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“…29 Chitosan is dissociated and excreted rapidly from the host body. 29 Conversely, pHEMA, a synthetic hydrogel, can resist hydrolytic and enzymatic degradation 30 and persists in the system for longer time. Therefore, we believed the development of a novel nanocomposite made from pHEMA (a strong depot core) surrounded by a chitosan shell (with target specificity) could provide a new, nonviral delivery system for biological applications.…”

Section: Discussionmentioning

confidence: 99%

A nonviral pHEMA+chitosan nanosphere-mediated high-efficiency gene delivery system

Eroğlu¹,

Tiwari²,

Waffo³

et al. 2013

IJN

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Abstract:The transport of DNA into eukaryotic cells is minimal because of the cell membrane barrier, and this limits the application of DNA vaccines, gene silencing, and gene therapy. Several available transfection reagents and techniques have been used to circumvent this problem. Alternatively, nonviral nanoscale vectors have been shown to bypass the eukaryotic cell membrane. In the present work, we developed a unique nanomaterial, pHEMA+chitosan nanospheres (PCNSs), which consisted of poly(2-hydroxyethyl methacrylate) nanospheres surrounded by a chitosan cationic shell, and we used this for encapsulation of a respiratory syncytial virus (RSV)-F gene construct (a model for a DNA vaccine). The new nanomaterial was capable of transfecting various eukaryotic cell lines without the use of a commercial transfection reagent. Using transmission electron microscopy, (TEM), fluorescence activated cell sorting (FACS), and immunofluorescence, we clearly demonstrated that the positively charged PCNSs were able to bind to the negatively charged cell membrane and were taken up by endocytosis, in Cos-7 cells. Using quantitative polymerase chain reaction (qPCR), we also evaluated the efficiency of transfection achieved with PCNSs and without the use of a liposomal-based transfection mediator, in Cos-7, HEp-2, and Vero cells. To assess the transfection efficiency of the PCNSs in vivo, these novel nanomaterials containing RSV-F gene were injected intramuscularly into BALB/c mice, resulting in high copy number of the transgene. In this study, we report, for the first time, the application of the PCNSs as a nanovehicle for gene delivery in vitro and in vivo.

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“…Poly(2-hidroxyethyl methacrylate)(PHEMA) have been widely used in the pharmaceutical industry because of biocompatibility and friendly characteristics [1][2][3][4][5][6][7]. It is interesting to study how the thermal stability of PHEMA of is affected by incorporating BisSi (bis-[trimethoxysilylpropyl] amine).…”

Section: Thermal Behavior Of Poly(2-hydroxyethyl Methacrylate-bis-[trmentioning

confidence: 99%

Thermal behavior of poly(2-hydroxyethyl methacrylate-bis-[trimethoxysilylpropyl]amine) networks

Figueroa¹,

Delgado²,

Hernández³

et al. 2013

IOP Conf. Ser.: Mater. Sci. Eng.

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Abstract. Poly(HEMA-BisSi) networks were prepared by using acid-catalyzed sol-gel of bis-[trimethoxysilylpropyl]amine (BisSi) and free radical polymerization of 2-hydroxyethyl methacrylate (HEMA). The thermal properties of the poly(HEMA -BisSi) networks were investigated with differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The thermal behavior of these networks was also compared with homopolymers (The networks formed in both PHEMA and PBisSi gels were identified). The glass transition temperature (T g ) of PHEMA homopolymer was found as 103.74 °C. The thermal degradation of the poly(HEMA-BisSi) networks with different silica contents (e.g. 10, 15 and 25 wt%) were evaluated with use of DTG. It was observed that the thermal degradation temperature of poly(HEMA-BisSi) networks changed much with the BisSi content.

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Synthesis and degradation of poly (2-hydroxyethyl methacrylate)-graft-poly (ε-caprolactone) copolymers

Cited by 49 publications

References 20 publications

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

A nonviral pHEMA+chitosan nanosphere-mediated high-efficiency gene delivery system

Thermal behavior of poly(2-hydroxyethyl methacrylate-bis-[trimethoxysilylpropyl]amine) networks

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