Synthesis and glycosylation of CD52, the major 'maturation-associated' antigen of rat spermatozoa, in the cauda epididymidis

Derr, P

doi:10.1530/reprod/121.3.435

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…In the same vein, the time required for capacitation was reduced in hamster spermatozoa obtained from a cauda suppressed by body temperature (Bedford & Yanagimachi, 1991), which reduces production of certain proteins (Esponda & Bedford, 1986), including the sperm coating glycopeptide, CD52 (Derr et al, 2001). That some human spermatozoa require a very brief capacitation period in vitro may reflect indications that the cauda epididymidis exists often in a temperature-suppressed state in man (Bedford, 1994).…”

Section: Enigmas Of Mammalian Gamete Form and Functionmentioning

confidence: 96%

“…Furthermore, although molecules such as the HIS protein and CD52 have been interpreted as relating to maturation, their appearance and binding to spermatozoa first in the upper cauda (after their maturation) suggests that these really facilitate sperm storage, as may similar molecules in other species (Dacheux & Voglmayr, 1983 ;Thomas, Reynolds & Oliphant, 1984;Garcia et al, 1988). That the rat periacrosomal surface then acquires two different proteins -the 100 kDa HIS dimer (Rifkin & Olson, 1985), and also a 26 kDa glycopeptide (Derr et al, 2001) -illustrates the present difficulty in assigning them a precise function in that regard.…”

Section: Indirect Determinants Of Gamete Functionmentioning

confidence: 99%

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Enigmas of mammalian gamete form and function

Bedford¹

2004

Biological Reviews

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The gametes of man and some other Eutheria have been manipulated successfully for practical reasons, but many gaps remain in our basic understanding of the way that they function. This situation stems not least from a failure to recognize the extent to which eutherian spermatozoa and eggs, and elements related to their operation, have come to differ from those of other groups. Novel features in the male that reflect this include a radical design of the sperm head with the acrosome seeming to function primarily in egg-coat binding rather than its lysis, a multifaceted post-testicular sperm maturation and an androgen/low-temperature-regulated system of sperm storage--both tied to the epididymis, a variable male accessory sex gland complex, and descent of the testis and epididymis to a scrotum. In the female, such novelties are represented in a need for sperm capacitation, in an unusual regulation of sperm transport within the oviduct, in the cumulus oophorus and character of the zona pellucida around the small egg, and in a unique configuration of gamete fusion. The collective evidence now suggests that many of these features reflect a new fertilisation strategy or its consequences, with most being causally linked. One initial 'domino' in this regard appears to be the small yolkless state of the egg and its intolerance for polyspermy, as determinants of the unusual mode of oviductal sperm transport and possibly the existence and form of the cumulus oophorus. However, a particularly influential first 'domino' appears to be the physical character of the eutherian zona pellucida. This differs from the egg coats of other animal groups by virtue of a resilient elasticity and thickness. These qualities allow this primary and often only coat to stretch and so persist during later expansion of the blastocyst, usually until close to implantation. At the same time, the dimensions, physical character, and particularly the relative protease-insensitivity of the zona appear to have had profound effects on sperm form and function and, more indirectly, on sperm-related events in the male and the female tract. Marsupials display some similarities and also some strikingly different features, against which the enigmas of the eutherian situation can be evaluated.

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Section: Enigmas Of Mammalian Gamete Form and Functionmentioning

confidence: 96%

Section: Indirect Determinants Of Gamete Functionmentioning

confidence: 99%

Enigmas of mammalian gamete form and function

Bedford¹

2004

Biological Reviews

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“…Although further experiments are necessary, human mrtCD52 seems to contain several O-linked carbohydrates different from the lymphocyte CD52. The rat counterpart of mrtCD52 has also been shown to contain extensive Olinked carbohydrate chains (Derr et al, 2001). The antiserum produced by immunization with the synthetic peptide of the CD52 core protein reacted to sperm and caused sperm agglutination and complement-dependent sperm immobilization.…”

Section: Discussionmentioning

confidence: 99%

Epitope analysis for human sperm-immobilizing monoclonal antibodies, MAb H6-3C4, 1G12 and campath-1

Hasegawa

Fu²,

Taniguchi³

et al. 2003

Molecular Human Reproduction

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Human monoclonal antibody, MAb H6-3C4, possesses strong sperm immobilizing activity. MAb H6-3C4 has been suggested by several research groups to react with a carbohydrate moiety of male reproductive tract CD52 (mrtCD52). In the present study, we analysed the epitope on mrtCD52 for MAb H6-3C4 and found that it was polymorphic in Western blot analysis and disappeared after enzymatic removal of the N-linked carbohydrate moiety. Two other monoclonal antibodies (1G12, campath-1) with sperm-immobilizing activity recognized mrtCD52 in a polymorphic manner similar to MAb H6-3C4. Further analysis showed that 1G12 recognized a structure formed by the peptide and/or a glycosylphosphatidylinositol (GPI) anchor portion as does campath-1. Results of a lectin binding assay suggested the presence of O-linked carbohydrates on mrtCD52. Our results also indicated that the peptide portion of CD52 could serve as an epitope for sperm-immobilizing antibodies. It was concluded that the epitope of MAb H6-3C4 is similar to, but distinct from, those of 1G12 and campath-1, and that mrtCD52 contains different antigenic epitopes.

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“…Inefficiency of mRNA translation has previously been described in the male genital tract, e.g., beta 2-adrenergic receptor mRNA in the prostate after castration [26] and SVS IV mRNA in seminal vesicles during the first stage of postnatal development [27]. A CD52 mRNA present in all parts of the epididymis in the rat is not efficiently translated in the cauda region, where the mRNA molecules possess a longer poly(A) tail [28,29]. No modifications in the length of PGDS mRNA were observed by Northern blotting at 4.5 mo compared with 4 mo of age, suggesting that modifications of poly(A) tail lengths may not be involved in this regulation of translation (Fig.…”

Section: Stage 3: Similarity In the Spatial Location Of Pgds Mrna Andmentioning

confidence: 99%

Prostaglandin D2 Synthase Secreted in the Caput Epididymidis Displays Spatial and Temporal Delay Between Messenger RNA and Protein Expression During Postnatal Development1

Fouchécourt

Castella

Dacheux

et al. 2003

Biology of Reproduction

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Highly regionalized protein secretory activity is established progressively throughout the epididymal tubule during postnatal development. Prostaglandin D(2) synthase (PGDS) is a major protein in the ovine epididymis, the secretion of which is restricted to the proximal part of the epididymis. We investigated the mRNA and protein expression of PGDS during ontogenesis. PGDS mRNA was present in the testis and epididymis in the 50-day-old sheep fetus and persisted until 4 mo of age (2 mo before puberty) without mRNA translation in the epididymis. At 4.5 mo, mRNA was present in all of the epididymis, but translation occurred in only the proximal caput. The secreted protein present in the lumen from the caput to the cauda had the same molecular mass and isoelectric point (pI) characteristics as the testicular form. At 5.5 mo, both mRNA and protein expression were restricted to the proximal caput. The protein secreted accumulated in the cauda but was not processed after secretion as it is in the adult animal; no changes in molecular mass or pI were observed. Our results show that for at least one gene (PGDS), transcriptional and posttranscriptional regulation during postnatal development is linked to the presence of unidentified translation factors present in testicular fluid.

show abstract

Synthesis and glycosylation of CD52, the major 'maturation-associated' antigen of rat spermatozoa, in the cauda epididymidis

Cited by 4 publications

References 19 publications

Enigmas of mammalian gamete form and function

Enigmas of mammalian gamete form and function

Epitope analysis for human sperm-immobilizing monoclonal antibodies, MAb H6-3C4, 1G12 and campath-1

Prostaglandin D2 Synthase Secreted in the Caput Epididymidis Displays Spatial and Temporal Delay Between Messenger RNA and Protein Expression During Postnatal Development1

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