Synthesis and in-Vitro Study of Some Medicinally Important Mannich Bases Derived From 2-Amino-9 [{(1, 3 Dihydroxy Propane-2yl) Oxy} Methyl] 6-9 Dihydro-3h-Purin-6-One

Abstract

A series of biologically active Mannich bases with heteroaromatic ring system have been synthesized employing Mannich reaction of 2-amino-9 [{(1,3 dihydroxy propane-2yl) oxy}methyl] 6-9 dihydro-3H-purin-6-one ( potent drug) with biologically active sulphonamides and secondary amines. They were analyzed by elemental analysis and characterized by spectral studies-UV, IR, 1 H NMR, Powder X-ray diffraction and Scanning Electron Microscopy. The Mannich bases were screened for their antibacterial activity against various gram + and gram -bacteria. The results had shown that the Mannich bases are quiet active against pathogens under study at varying concentrations. The toxicity of synthesized Mannich bases was ascertained by LD 50 test.

Synthesis of New Antibiotics via Mannish Reaction

“…Amount of 0.4mL (0.015mol) of formaldehyde solution (37%, v/v) was added slowly with constant stirring. The reaction mixture was stirred at 70-75 °C for 3 to 8.5 hours, depending upon the secondary amine [5]. The remaining portion of formaldehyde solution was added in two installments after 1 and 2 hours, respectively.…”