Synthesis and sensitive detection of doxycycline with sodium bis 2-ethylhexylsulfosuccinate based silver nanoparticle

Siddiqui, Asma; Anwar, Humera; Ahmed, Syed Waseem; Naqvi, Syeda Sumra; Shah, Mohammad Raza; Ahmed, Ayaz; Ali, Syed Abid

doi:10.1016/j.saa.2019.117489

Cited by 27 publications

(5 citation statements)

References 62 publications

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“…As shown in Figure a, a high-intensity surface plasmon resonance band was observed at ∼460, ∼429, ∼430, and ∼433 nm for the bAgNPs synthesized at pH 5, 7, 8, and 10, respectively, which are well within the characteristic wavelength range (λ max ) of synthesized AgNPs. , Furthermore, the intensity of the SPR band of bAgNPs increased with the increasing pH ( i.e ., 5–8) and, thereafter, decreased at higher pH ( i.e ., >8) (Figure a). The SPR band intensity of bAgNPs synthesized at pH 8 was the highest among all of the synthesized nanoparticles, indicating the highest intensity of bAgNPs at this pH − (Figure a). Furthermore, the absorption spectra of bAgNPs synthesized at pH 5 showed a significant red shift of the SPR absorption band ( i.e ., shifted to higher wavelengths (460 nm)), indicating a low intensity as well as a distinct morphology ( i.e ., size and shape) of the synthesized nanoparticles. , The bAgNPs synthesized at pH 10 also showed a red shift of the SPR absorption band, suggesting a decrease in the intensity of nanoparticles at pH beyond 8.…”

Section: Resultsmentioning

confidence: 94%

Synthesis of Biogenic Silver Nanoparticles Using Caesalpinia digyna and Investigation of Their Antimicrobial Activity and In Vivo Biocompatibility

Niloy

Hossain

Takikawa

et al. 2020

ACS Appl. Bio Mater.

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Among metallic nanoparticles, silver nanoparticles (AgNPs) have a wide spectrum of medical applications. Herein, biogenic silver nanoparticles (bAgNPs) were prepared from extracts of Caesalpinia digyna leaf as a reducing agent at different pH values (i.e., 5, 7, 8, and 10). The as-synthesized bAgNPs were characterized using UV–vis and Fourier transform infrared (FTIR) spectroscopies, scanning transmission electron microscopy, powder X-ray diffraction analysis, dynamic light scattering, and ζ-potential analysis. The sizes of bAgNPs prepared at pH 5, 7, 8, and 10 were 45.4, 11.3, 11.4, and 40.8 nm, respectively, and all of the nanoparticles were negatively charged. The antimicrobial activity of the as-prepared bAgNPs was investigated against Bacillus subtilis, Escherichia coli DH5α, E. coli K12, enteropathogenic E. coli (EPEC), and Salmonella typhi. The bAgNPs prepared at pH 8 showed the highest antibacterial propensity against all of the bacterial strains as exhibited in the zone of inhibition (ZOI) as well as the CellTox green assay, which can be due to their relatively small size, stability, and higher surface area-to-volume ratio. The bAgNPs synthesized at pH 8 showed the highest ZOI against B. subtilis, which was ∼25 mm in diameter. The lipid peroxidation assay demonstrated the formation of the malondialdehyde-thiobarbituric acid (MDA-TBA) adduct while treating the bacteria with bAgNPs due to the oxidation of fatty acids present in the membrane. The highest amount of MDA-TBA adduct was observed when Gram-positive B. subtilis was exposed to bAgNPs. On the contrary, rats treated with bAgNPs demonstrated no significant toxicity in terms of hematological and biochemical parameters. The bAgNPs also showed excellent compatibility with human red blood cells. Overall, bAgNPs synthesized at pH 8 have superior antimicrobial activity and excellent biocompatibility and, therefore, can be used as potential antibacterial agents.

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Section: Resultsmentioning

confidence: 94%

Synthesis of Biogenic Silver Nanoparticles Using Caesalpinia digyna and Investigation of Their Antimicrobial Activity and In Vivo Biocompatibility

Niloy

Hossain

Takikawa

et al. 2020

ACS Appl. Bio Mater.

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“…In addition, there appears also a bunch of small absorbance peaks from 620 to 1040 cm –1 , from 1120 to 1230 cm –1 , and from 1330 to 1456 cm –1 , which were likely caused by C–H bonds on the phenyl group, C–O bonds, and C–H bending vibration in different methyl and methene groups, respectively. Seeing the slight differences of these groups, it is not unexpected that they appear as multiple and very close absorbance peaks. − It is noted that the intensities of these peaks are hardly perceptible as long as [DOX] did not exceed 10 3 μM and become more pronounced with [DOX] increased to 10 5 μM (Figure S3A). This is well evidenced by comparing the DOX spectrum to those of P8k and the binary solution (Figure S3B).…”

Section: Resultsmentioning

confidence: 99%

Full Green Detection of Antibiotic Tetracyclines Using Fluorescent Poly(ethylene glycol) as the Sensor and the Mechanism Study

Wang

Jiang

Sun

et al. 2022

ACS Biomater. Sci. Eng.

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Tetracyclines are well-known antibiotics and widely used against a variety of bacterial infections. Their monitoring and detection have been an important issue. To this end, a vast number of methods have been developed; fluorescence sensing is one of the most reported. However, most of the reported sensors are made from transition metals with sophisticated multiprocesses; polymers are hardly seen for this purpose, particularly biocompatible ones. Herein, an aqueous solution of poly(ethylene glycol) (PEG), well known for being biocompatible, is shown to emit under excitation of 280 nm, while the solutions of selected tetracyclines, namely, doxycycline (DOX) and tetracycline (TC), are non-emissive under the same conditions. In the binary solutions of PEG-DOX or PEG-TC, PEG emission is sharply quenched with high sensitivity and selectivity. PEG was then used as a sensor for DOX and TC detections in water with high performance compared to reported studies. The same tests were also done by DOX spiking in milk and tap water, demonstrating that DOX was practically fully recovered. The quenching mechanism was ascribed to the interaction between the O atoms of PEG in clusters and specific heteroatom groups on tetracycline molecules through hydrogen bonding, elucidated from FTIR and NMR analyses. Therefore, this work provides a novel, fully green, easy to operate, low cost, and reliable protocol for tetracycline monitoring and detection and opens new potential application for PEG.

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“…As for Gramnegative bacteria, in the study conducted by Lotha et al (2018), the MBIC 50 varied between 16 and 32 µg/mL. In the studies of Siddiqui et al (2020), who synthesized AgNPs by chemical reduction, there was no significant difference between MBIC values obtained compared to their bacterial groups used (MBIC ranging from 75 to 150 µg/mL). The study included Gram-positive bacteria (S. aureus and S. epidermidis) and Gram-negative bacteria (P. aeruginosa and Klebsiella pneumoniae).…”

Section: Evaluation Of Antibiofilm Activitymentioning

confidence: 97%

Silver nanoparticles–chitosan composites activity against resistant bacteria: tolerance and biofilm inhibition

et al. 2021

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This study aimed to evaluate the effectiveness of silver nanoparticles–chitosan composites (AgNPs) with different morphologies and particle size distributions against resistant bacteria and biofilm formation. Four different samples were prepared by a two-step procedure using sodium borohydride and ascorbic acid as reducing agents and characterized by UV–Vis absorption spectra, scanning transmission electron microscopy. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the AgNPs were determined according to the Clinical and Laboratory Standards Institute (CLSI) against clinical isolates multidrug-resistant and strains of the American Type Culture Collection (ATCC). An assay was performed to determine the MICs during 20 successive bacteria exposures to AgNPs to investigate whether AgNPs induce tolerance in bacteria. The antibiofilm activities of AgNPs were also evaluated by determining the minimum biofilm inhibitory concentration (MBIC). The spherical AgNPs present diameters ranging from 9.3 to 62.4 nm, and some samples also have rod-, oval-, and triangle-shaped nanoparticles. The MIC and MBC values ranged from 0.8 to 25 μg/mL and 3.1 to 50 μg/mL, respectively. Smaller and spherical AgNPs exhibited the highest activity, but all the AgNPs developed in this study exhibit bactericidal activity. There was no significant MIC increase after 20 passages to the AgNPs. Regarding the antibiofilm activity, MBICs ranged from 12.5 to 50 μg/mL. Again, smaller and spherical nanoparticles presented the best results with phenotypic inhibition of production of slime or exopolysaccharide (EPS) matrix. Thus, it was concluded that AgNPs have a promising potential against resistant bacteria and bacteria that grow on biofilms without inducing tolerance. Supplementary Information The online version contains supplementary material available at 10.1007/s11051-021-05314-1.

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Synthesis and sensitive detection of doxycycline with sodium bis 2-ethylhexylsulfosuccinate based silver nanoparticle

Cited by 27 publications

References 62 publications

Synthesis of Biogenic Silver Nanoparticles Using Caesalpinia digyna and Investigation of Their Antimicrobial Activity and In Vivo Biocompatibility

Synthesis of Biogenic Silver Nanoparticles Using Caesalpinia digyna and Investigation of Their Antimicrobial Activity and In Vivo Biocompatibility

Full Green Detection of Antibiotic Tetracyclines Using Fluorescent Poly(ethylene glycol) as the Sensor and the Mechanism Study

Silver nanoparticles–chitosan composites activity against resistant bacteria: tolerance and biofilm inhibition

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