Synthesis and Structure of Arene Ru(II) N<sup>∧</sup>O-Chelating Complexes: <i>In Vitro</i> Cytotoxicity and Cancer Cell Death Mechanism

Balaji, S.; Subarkhan, Mohamed Kasim Mohamed; Ramesh, Rengan; Wang, Hangxiang; Sémeril, David

doi:10.1021/acs.organomet.0c00092

Cited by 153 publications

(63 citation statements)

References 71 publications

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“…The reduced cell viability on day 1 was due to the presence of free Ce-nGel with the NIH3T3 fibroblasts. After day 1, the remaining viable fibroblasts multiplied, consequently improving the viability (Subarkhan & Ramesh, Kasim et al, 2018;Mohamed Subarkhan et al, 2019;Balaji et al, 2020;Sathiya Kamatchi et al, 2020).…”

Section: Antibacterial and Cell Viability Analysesmentioning

confidence: 99%

RETRACTED ARTICLE: Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery

et al. 2021

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This study was designed to establish the composition of wound bandages based on Cerium nanoparticle (CeNP)-loaded polyvinyl alcohol (PVA) nanogels. The CeNP nanogel (Ce-nGel) was fabricated by the fructose-mediated reduction of Cerium oxide solutions within the PVA matrix. The influences of different experimental limitations on PVA nanogel formations were examined. The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from $10 to 50 nm. Additionally, glycerol was added to the Ce-nGels, and the resulting compositions (Ce-nGel-Glu) were coated on cotton fabrics to generate the wound bandaging composite. The cumulative drug release profile of the Cerium from the bandage was found to be $38% of the total loading after two days. Additionally, antibacterial efficacy was developed for Gam positive and negative microorganisms. Moreover, we examined in vivo healing of skin wounds formed in mouse models over 24 days. In contrast to the untreated wounds, rapid healing was perceived in the Ce-nGel-Glu-treated wound with less damage. These findings indicate that Ce-nGel-Glu-based bandaging materials could be a potential candidate for wound healing applications in the future.

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Section: Antibacterial and Cell Viability Analysesmentioning

confidence: 99%

RETRACTED ARTICLE: Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery

et al. 2021

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“…d 6 transition metals show useful photophysical properties, including visible-light absorption and emission, chemical stability, and low cytotoxicity ( Fernandez-Moreira et al, 2010 ; Thorp-Greenwood et al, 2012 ; Carreño et al, 2019 ; Otero et al, 2019 ; Carreño et al, 2021 ). In addition, d 6 transition-metal complexes have shown interesting biological applications not only as fluorophores but also in other areas such as selective cytotoxic complexes against cancer cells ( Tian et al, 2017 ; Xu et al, 2018 ; Balaji et al, 2020 ; Li et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

New Cationic fac-[Re(CO)3(deeb)B2]+ Complex, Where B2 Is a Benzimidazole Derivative, as a Potential New Luminescent Dye for Proteins Separated by SDS-PAGE

et al. 2021

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Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be used to separate proteins based mainly on their size such as in denaturing gels. Different staining methods have been reported to observe proteins in the gel matrix, where the most used dyes are generally anionic. Anionic dyes allow for interactions with protonated amino acids, retaining the dye in the proteins. Fluorescent staining is an alternative technique considered to be sensitive, safe, and versatile. Some anionic complexes based on d6 transition metals have been used for this purpose, where cationic dyes have been less explored in this context. In this work, we synthesized and characterized a new monocationic rhenium complex fac-[Re(CO)3(deeb)B2]+ (where deeb is 4,4′-bis(ethoxycarbonyl)-2,2′-bpy and B2 is 2,4-di-tert-butyl-6-(3H-imidazo[4,5-c]pyridine-2-yl)phenol). We carried out a structural characterization of this complex by MS+, FTIR, 1H NMR, D2O exchange, and HHCOSY. Moreover, we carried out UV-Vis, luminescence, and cyclic voltammetry experiments to understand the effect of ligands on the complex’s electronic structure. We also performed relativistic theoretical calculations using the B3LYP/TZ2P level of theory and R-TDDFT within a dielectric continuum model (COSMO) to better understand electronic transitions and optical properties. We finally assessed the potential of fac-[Re(CO)3(deeb)B2]+ (as well as the precursor fac-Re(CO)3(deeb)Br and the free ligand B2) to stain proteins separated by SDS-PAGE. We found that only fac-[Re(CO)3(deeb)B2]+ proved viable to be directly used as a luminescent dye for proteins, presumably due to its interaction with negatively charged residues in proteins and by weak interactions provided by B2. In addition, fac-[Re(CO)3(deeb)B2]+ seems to interact preferentially with proteins and not with the gel matrix despite the presence of sodium dodecyl sulfate (SDS). In future applications, these alternative cationic complexes might be used alone or in combination with more traditional anionic compounds to generate counterion dye stains to improve the process.

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“…The cell apoptosis assay grouping technique was in accordance with the cell viability assay group. After administering IC 50 concentration of the formulations of Gd-PFH-NAs and C-Gd-PFH-NAs was implemented 2 h later (Mohan et al., 2018 ; Mohamed Subarkhan et al., 2019 ; Balaji et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Precise engineering of cetuximab encapsulated gadollium nanoassemblies: in vitro ultrasound diagnosis and in vivo thyroid cancer therapy

Qi¹,

Gao²,

Nie³

et al. 2021

Drug Delivery

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We report the formulation of nanoassemblies (NAs) comprising C225 conjugates Gd-PFH-NAs (C-Gd-PFH-NAs) for low-intensity focused ultrasound diagnosis ablation of thyroid cancer. C-Gd-PFH-NAs showed excellent stability in water, phosphate-buffered saline (PBS), and 20% rat serum. Transmission electron microscopy (TEM) images also revealed the effective construction of C-Gd-PFH-NAs as common spherical assemblies. The incubation of C625 thyroid carcinoma with C-Gd-PFH-NAs triggers apoptosis, as confirmed by flow cytometry analysis. The C-Gd-PFH-NAs exhibited antitumor efficacy in human thyroid carcinoma xenografts, where histopathological results further confirmed these outcomes. Furthermore, we were able to use low-intensity focused ultrasound diagnosis imaging (LIFUS) to examine the efficiency of C-Gd-PFH-NAs in thyroid carcinoma in vivo. These findings clearly show that the use of LIFUS agents with high performance imaging in different therapeutic settings will have extensive potential for future biomedical applications.

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Synthesis and Structure of Arene Ru(II) N^∧O-Chelating Complexes: In Vitro Cytotoxicity and Cancer Cell Death Mechanism

Cited by 153 publications

References 71 publications

RETRACTED ARTICLE: Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery

RETRACTED ARTICLE: Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery

New Cationic fac-[Re(CO)3(deeb)B2]+ Complex, Where B2 Is a Benzimidazole Derivative, as a Potential New Luminescent Dye for Proteins Separated by SDS-PAGE

Precise engineering of cetuximab encapsulated gadollium nanoassemblies: in vitro ultrasound diagnosis and in vivo thyroid cancer therapy

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Synthesis and Structure of Arene Ru(II) N∧O-Chelating Complexes: In Vitro Cytotoxicity and Cancer Cell Death Mechanism

Cited by 153 publications

References 71 publications

RETRACTED ARTICLE: Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery

RETRACTED ARTICLE: Cerium oxide nanoparticle-loaded polyvinyl alcohol nanogels delivery for wound healing care systems on surgery

New Cationic fac-[Re(CO)3(deeb)B2]+ Complex, Where B2 Is a Benzimidazole Derivative, as a Potential New Luminescent Dye for Proteins Separated by SDS-PAGE

Precise engineering of cetuximab encapsulated gadollium nanoassemblies: in vitro ultrasound diagnosis and in vivo thyroid cancer therapy

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Synthesis and Structure of Arene Ru(II) N^∧O-Chelating Complexes: In Vitro Cytotoxicity and Cancer Cell Death Mechanism